Custom CTAG fusions domestication using the former GB Syntax
Category CDS1+CDS2 (B3-B4) domestication:
- Upload your sequence to the “Domestication” tool. Please, note that since you are going to perform a custom domestication your input sequence should NOT include the initial ATG.
- Leave the “Choose a category to domesticate to” field empty.
- Fill “Custom prefix” = AATG and “Custom suffix” = GCAG (click here for a comparison between the old and new syntax)
Category CTAG (B5) domestication:
- Add nucleotides GG to the 5’end of your sequence (you need to add two bases in order to keep the ORF). Keep in mind that your sequence should include a STOP CODON at the 3'end.
- Upload your sequence to the “Domestication” tool.
- Leave the “Choose a category to domesticate to” field empty.
- Fill “Custom prefix” = GCAG and “Custom suffix” = GCTT (click here for a comparison between the old and new syntax)
- To add your domesticated parts to the GB database select "Type": OTHER (note that you cannot add your parts as standard parts since they do not adapt to the standard grammar).
Assembly of a CT-fusion Transcriptional Unit with "old" GBparts:
You cannot use the standard GB TU Assembler with non-standard parts. To assemble a CT-fusion TU using "old" GBparts:
- Go to the "GB TU Free Assembler" tool and follow the instructions thereof.
