Custom intron (B4) / iog (B5) domestication using the former GB Syntax
Category Intron (B4) domestication:
- Upload your sequence to the “Domestication” tool.
- Leave the “Choose a category to domesticate to” field empty.
- Fill “Custom prefix” = AGCC and “Custom suffix” = GCAG
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Category IOG (B5) domestication:
- Upload your sequence to the “Domestication” tool.
- Leave the “Choose a category to domesticate to” field empty.
- Fill “Custom prefix” = GCAG and “Custom suffix” = GCTT
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- To add your domesticated parts to the GB database select "Type": OTHER (note that you cannot add your parts as standard parts since they do not adapt to the standard grammar).
Assembly of a hpRNA Transcriptional Unit with "old" GBparts:
You cannot use the standard GB TU Assembler with non-standard parts. To assemble a hpRNA TU using "old" GBparts:
- Go to the "GB TU Free Assembler" tool and follow the instructions thereof.