Custom intron (B4) / iog (B5) domestication using the former GB Syntax

Category Intron (B4) domestication:

  • Upload your sequence to the “Domestication” tool.
  • Leave the “Choose a category to domesticate to” field empty.
  • Fill “Custom prefix” = AGCC and “Custom suffix” = GCAG

Category IOG (B5) domestication:

  • Upload your sequence to the “Domestication” tool.
  • Leave the “Choose a category to domesticate to” field empty.
  • Fill “Custom prefix” = GCAG and “Custom suffix” = GCTT

- To add your domesticated parts to the GB database select "Type": OTHER (note that you cannot add your parts as standard parts since they do not adapt to the standard grammar).



Assembly of a hpRNA Transcriptional Unit with "old" GBparts:

You cannot use the standard GB TU Assembler with non-standard parts. To assemble a hpRNA TU using "old" GBparts: