Single Cas12a gRNA Assembler (tS12A)
Choose the U6-26 promoter-direct repeat you want to use. At the moment we have available U6-26-direct repeat fusions for AsCas12a and LbCas12a. Select your own domesticated target sequence, a 3' processing signal (HDV is the only one available at the moment in our public collection), a destination vector and proceed with the assembly.