Multiple Cas12a gRNA domesticator 5x (tM12D)

CRISPR Domestication

Type or paste your 20-23 nt TARGET 1 sequence here:
Type or paste your 20-23 nt TARGET 2 sequence here:
Type or paste your 20-23 nt TARGET 3 sequence here:
Type or paste your 20-23 nt TARGET 4 sequence here:
Type or paste your 20-23 nt TARGET 5 sequence here:
'The CRISPR domesticator tool will produce a domesticated sgRNA sequence flanked by standard GB overhangs (prefix and suffix). If you prefer to create your own non-standard flanking 4 nucleotide overhangs, please provide them in the boxes below (optional). Please note that if you choose the custom option the resulting oligonucleotides will not assemble with the rest of GB standard CRISPR elements.'
Custom prefix:
Custom suffix:

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