LOCUS       GB0152               5258 bp    DNA              UNK 01-JAN-1980
DEFINITION  (GB0094,GB0050,GB0096,GB0037)pDGB1_alpha2
ACCESSION   GB0152
VERSION     GB0152
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..70
                     /vntifkey="21"
                     /label="Op"
     misc_feature    77..122
                     /vntifkey="21"
                     /label="Op"
     misc_feature    129..174
                     /vntifkey="21"
                     /label="Op"
     misc_feature    181..226
                     /vntifkey="21"
                     /label="Op"
     misc_feature    233..278
                     /vntifkey="21"
                     /label="Op"
     misc_feature    285..330
                     /vntifkey="21"
                     /label="Op"
     gene            506..2158
                     /note="LUC"
                     /vntifkey="60"
                     /gene="luc"
     CDS             506..2158
                     /product="luciferase"
                     /codon_start=1
                     /label="Firefly\Luciferase"
                     /transl_table=11
                     /note="firefly luciferase"
                     /db_xref="GI:155733599"
                     /vntifkey="4"
                     /gene="luc"
                     /protein_id="ABU39926.1"
     mutation        2246
                     /vntifkey="62"
                     /label="T>C"
     terminator      2162..2657
                     /vntifkey="43"
                     /label="Tnos"
     misc_feature    2658..2661
                     /vntifkey="21"
                     /label="CGCT"
     repeat_region   2674..2698
                     /vntifkey="34"
                     /label="RB"
     CDS             3544..4367
                     /vntifkey="4"
                     /label="KanR"
     repeat_region   5218..5241
                     /vntifkey="34"
                     /label="LB"
     rep_origin      4649..5201
                     /vntifkey="33"
                     /label="pSa"
     rep_origin      complement(2724..3448)
                     /vntifkey="33"
                     /label="pUC-ori"
     misc_feature    5255..5258
                     /vntifkey="21"
                     /label="GTCA"
     misc_feature    5238..5241
                     /vntifkey="21"
                     /label="TAAC"
     
ORIGIN
        1 ggagttgcga agatcgatcc actagtcttt caattgtgag cgctcacaat tctttctctt
       61 ccctttcttc tttctagtct ttcaattgtg agcgctcaca attctttctc ttccctttct
      121 tctttctagt ctttcaattg tgagcgctca caattctttc tcttcccttt cttctttcta
      181 gtctttcaat tgtgagcgct cacaattctt tctcttccct ttcttctttc tagtctttca
      241 attgtgagcg ctcacaattc tttctcttcc ctttcttctt tcttgtcttt caattgtgag
      301 cgctcacaat tctttctctt ccctttcttc tttctagctc caccgcggtg gcggccggcc
      361 gctctagtgg atcgattccc cttcgcaaga cccttcctct atataaggaa gttcatttca
      421 tttggagagg actccggtat ttttacaaca attaccacaa caaaacaaac aacaaacaac
      481 attacaattt actattctag tcgaaatgga agacgccaaa aacataaaga aaggcccggc
      541 gccattctat ccgctggaag atggaaccgc tggagagcaa ctgcataagg ctatgaagag
      601 atacgccctg gttcctggaa caattgcttt tacagatgca catatcgagg tggacatcac
      661 ttacgctgag tacttcgaaa tgtccgttcg gttggcagaa gctatgaaac gatatgggct
      721 gaatacaaat cacagaatcg tcgtatgcag tgaaaactct cttcaattct ttatgccggt
      781 gttgggcgcg ttatttatcg gagttgcagt tgcgcccgcg aacgacattt ataatgaacg
      841 tgaattgctc aacagtatgg gcatttcgca gcctaccgtg gtgttcgttt ccaaaaaggg
      901 gttgcaaaaa attttgaacg tgcaaaaaaa gctcccaatc atccaaaaaa ttattatcat
      961 ggattctaaa acggattacc agggatttca gtcgatgtac acgttcgtca catctcatct
     1021 acctcccggt tttaatgaat acgattttgt gccagagtcc ttcgataggg acaagacaat
     1081 tgcactgatc atgaactcct ctggatctac tggtctgcct aaaggtgtcg ctctgcctca
     1141 tagaactgcc tgcgtgagat tctcgcatgc cagagatcct atttttggca atcaaatcat
     1201 tccggatact gcgattttaa gtgttgttcc attccatcac ggttttggaa tgtttactac
     1261 actcggatat ttgatatgtg gatttcgagt cgtcttaatg tatagatttg aagaagagct
     1321 gtttctgagg agccttcagg attacaagat tcaaagtgcg ctgctggtgc caaccctatt
     1381 ctccttcttc gccaaaagca ctctgattga caaatacgat ttatctaatt tacacgaaat
     1441 tgcttctggt ggcgctcccc tctctaagga agtcggggaa gcggttgcca agaggttcca
     1501 tctgccaggt atcaggcaag gatatgggct cactgagact acatcagcta ttctgattac
     1561 acccgagggg gatgataaac cgggcgcggt cggtaaagtt gttccatttt ttgaagcgaa
     1621 ggttgtggat ctggataccg ggaaaacgct gggcgttaat caaagaggcg aactgtgtgt
     1681 gagaggtcct atgattatgt ccggttatgt aaacaatccg gaagcgacca acgccttgat
     1741 tgacaaggat ggatggctac attctggaga catagcttac tgggacgaag acgaacactt
     1801 cttcatcgtt gaccgcctga agtctctgat taagtacaaa ggctatcagg tggctcccgc
     1861 tgaattggaa tccatcttgc tccaacaccc caacatcttc gacgcaggtg tcgcaggtct
     1921 tcccgacgat gacgccggtg aacttcccgc cgccgttgtt gttttggagc acggaaagac
     1981 gatgacggaa aaagagatcg tggattacgt cgccagtcaa gtaacaaccg cgaaaaagtt
     2041 gcgcggagga gttgtgtttg tggacgaagt accgaaaggt cttaccggaa aactcgacgc
     2101 aagaaaaatc agagagatcc tcataaaggc caagaagggc ggaaagatcg ccgtgtaatg
     2161 agcttggaat ggatcttcga tcccgatcgt tcaaacattt ggcaataaag tttcttaaga
     2221 ttgaatcctg ttgccggtct tgcgacgatt atcatataat ttctgttgaa ttacgttaag
     2281 catgtaataa ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga
     2341 gtcccgcaat tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat
     2401 aaattatcgc gcdcggtgtc atctatgtta ctagatcggg aattgccaag ctaattcttg
     2461 aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     2521 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     2581 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     2641 ataatgggac cgactcgcgc ttgagacgaa gcttgacagg atatattggc gggtaaacta
     2701 agtcgctgta tgtgtttgtt tgagatctca tgtgagcaaa aggccagcaa aaggccagga
     2761 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     2821 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     2881 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     2941 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     3001 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     3061 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     3121 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     3181 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     3241 gtatctgcgc tctgctgaag ccagttacct tcggaagaag agttggtagc tcttgatccg
     3301 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     3361 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     3421 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     3481 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgtg taacattggt
     3541 ctagtgatta gaaaaactca tcgagcatca aatgaaactg caatttattc atatcaggat
     3601 tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc
     3661 agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa
     3721 tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag
     3781 tgacgactga atccggtgag aatggcaaaa gtttatgcat ttctttccag acttgttcaa
     3841 caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg ttattcattc
     3901 gtgattgcgc ctgagcgagt cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag
     3961 gaatcgaatg caaccggcgc aggaacactg ccagcgcatc aacaatattt tcacctgaat
     4021 caggatattc ttctaatacc tggaatgctg ttttccctgg gatcgcagtg gtgagtaacc
     4081 atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagaggcata aattccgtca
     4141 gccagtttag tctgaccatc tcatctgtaa caacattggc aacgctacct ttgccatgtt
     4201 tcagaaacaa ctctggcgca tcgggcttcc catacaatcg gtagattgtc gcacctgatt
     4261 gcccgacatt atcgcgagcc catttatacc catataaatc agcatccatg ttggaattta
     4321 atcgcggcct tgagcaagac gtttcccgtt gaatatggct cataacaccc cttgtattac
     4381 tgtttatgta agcagacagt tttattgttc atgatgatat atttttatct tgtgcaatgt
     4441 aacatcagag attttgagac acaacgtggc tttgttgaat aaatcgaact tttgctgagt
     4501 tgaaggatca gatcacgcat cttcccgaca acgcagaccg ttccgtggca aagcaaaagt
     4561 tcaaaatcac caactggtcc acctacaaca aagctctcat caaccgtggc tccctcactt
     4621 tctggctgga tgatggggcg attcaggcga tccccatcca acagcccgcc gtcgagcggg
     4681 cttttttatc cccggaagcc tgtggataga gggtagttat ccacgtgaaa ccgctaatgc
     4741 cccgcaaagc cttgattcac ggggctttcc ggcccgctcc aaaaactatc cacgtgaaat
     4801 cgctaatcag ggtacgtgaa atcgctaatc ggagtacgtg aaatcgctaa taaggtcacg
     4861 tgaaatcgct aatcaaaaag gcacgtgaga acgctaatag ccctttcaga tcaacagctt
     4921 gcaaacaccc ctcgctccgg caagtagtta cagcaagtag tatgttcaat tagcttttca
     4981 attatgaata tatatatcaa ttattggtcg cccttggctt gtggacaatg cgctacgcgc
     5041 accggctccg cccgtggaca accgcaagcg gttgcccacc gtcgagcgcc tttgcccaca
     5101 acccggcggc cggccgcaac agatcgtttt ataaattttt ttttttgaaa aagaaaaagc
     5161 ccgaaaggcg gcaacctctc gggcttctgg atttccgatc cccggaatta gagatcttgg
     5221 caggatatat tgtggtgtaa caagcttcgt ctcagtca
//
