LOCUS       GB0575             7196 bp    DNA     circular     22-MAR-2015
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|700428410|
COMMENT     VNTDBDATE|729090616|
COMMENT     LSOWNER|
COMMENT     VNTNAME|phCas9_pUPD|
COMMENT     VNTAUTHORNAME|Marta|
FEATURES             Location/Qualifiers
     CDS             complement(1295..2155)
                     /vntifkey="4"
                     /label=AmpR
     mutation        1436..1436
                     /vntifkey="62"
                     /label=A>C
     mutation        2551..2551
                     /vntifkey="62"
                     /label=A>C
     CDS             3053..7189
                     /vntifkey="4"
                     /label=hCas9
     misc_feature    7169..7189
                     /vntifkey="21"
                     /label=SV40\NLS
BASE COUNT     2070 a      1728 c      1789 g      1609 t 
ORIGIN
        1 cgagaccact catcgcccat cactagtgaa ttcgcggccg cctgcaggtc gaccatatgg 
       61 gagagctccc aacgcgttgg atgcatagct tgagtattct atagtgtcac ctaaatagct 
      121 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac 
      181 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac 
      241 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc 
      301 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg 
      361 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 
      421 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 
      481 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 
      541 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 
      601 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 
      661 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 
      721 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 
      781 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 
      841 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 
      901 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 
      961 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 
     1021 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 
     1081 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 
     1141 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 
     1201 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 
     1261 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 
     1321 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 
     1381 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgcgacc 
     1441 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 
     1501 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 
     1561 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 
     1621 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 
     1681 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 
     1741 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 
     1801 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 
     1861 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 
     1921 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 
     1981 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 
     2041 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 
     2101 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 
     2161 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 
     2221 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 
     2281 cacctgatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggaaa 
     2341 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt 
     2401 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag 
     2461 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg 
     2521 tcaaagggcg aaaaaccgtc tatcagggcg ctggcccact acgtgaacca tcaccctaat 
     2581 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc 
     2641 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga 
     2701 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac 
     2761 ccgccgcgct taatgcgccg ctacagggcg cgtccattcg ccattcaggc tgcgcaactg 
     2821 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg 
     2881 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac 
     2941 gacggccagt gaattgtaat acgactcact atagggcgaa ttgggcccga cgtcgcatgc 
     3001 tcccggccgc catggcggcc gcgggaattc gatgggcgat gagtggtctc gaatggacaa 
     3061 gaagtactcc attgggctcg atatcggcac aaacagcgtc ggctgggccg tcattacgga 
     3121 cgagtacaag gtgccgagca aaaaattcaa agttctgggc aataccgatc gccacagcat 
     3181 aaagaagaac ctcattggcg ccctcctgtt cgactccggg gaaacggccg aagccacgcg 
     3241 gctcaaaaga acagcacggc gcagatatac ccgcagaaag aatcggatct gctacctgca 
     3301 ggagatcttt agtaatgaga tggctaaggt ggatgactct ttcttccata ggctggagga 
     3361 gtcctttttg gtggaggagg ataaaaagca cgagcgccac ccaatctttg gcaatatcgt 
     3421 ggacgaggtg gcgtaccatg aaaagtaccc aaccatatat catctgagga agaagcttgt 
     3481 agacagtact gataaggctg acttgcggtt gatctatctc gcgctggcgc atatgatcaa 
     3541 atttcgggga cacttcctca tcgaggggga cctgaaccca gacaacagcg atgtcgacaa 
     3601 actctttatc caactggttc agacttacaa tcagcttttc gaagagaacc cgatcaacgc 
     3661 atccggagtt gacgccaaag caatcctgag cgctaggctg tccaaatccc ggcggctcga 
     3721 aaacctcatc gcacagctcc ctggggagaa gaagaacggc ctgtttggta atcttatcgc 
     3781 cctgtcactc gggctgaccc ccaactttaa atctaacttc gacctggccg aagatgccaa 
     3841 gcttcaactg agcaaagaca cctacgatga tgatctcgac aatctgctgg cccagatcgg 
     3901 cgaccagtac gcagaccttt ttttggcggc aaagaacctg tcagacgcca ttctgctgag 
     3961 tgatattctg cgagtgaaca cggagatcac caaagctccg ctgagcgcta gtatgatcaa 
     4021 gcgctatgat gagcaccacc aagacttgac tttgctgaag gcccttgtca gacagcaact 
     4081 gcctgagaag tacaaggaaa ttttcttcga tcagtctaaa aatggctacg ccggatacat 
     4141 tgacggcgga gcaagccagg aggaatttta caaatttatt aagcccatct tggaaaaaat 
     4201 ggacggcacc gaggagctgc tggtaaagct taacagagaa gatctgttgc gcaaacagcg 
     4261 cactttcgac aatggaagca tcccccacca gattcacctg ggcgaactgc acgctatcct 
     4321 caggcggcaa gaggatttct accccttttt gaaagataac agggaaaaga ttgagaaaat 
     4381 cctcacattt cggataccct actatgtagg ccccctcgcc cggggaaatt ccagattcgc 
     4441 gtggatgact cgcaaatcag aagagactat cactccctgg aacttcgagg aagtcgtgga 
     4501 taagggggcc tctgcccagt ccttcatcga aaggatgact aactttgata aaaatctgcc 
     4561 taacgaaaag gtgcttccta aacactctct gctgtacgag tacttcacag tttataacga 
     4621 gctcaccaag gtcaaatacg tcacagaagg gatgagaaag ccagcattcc tgtctggaga 
     4681 gcagaagaaa gctatcgtgg acctcctctt caagacgaac cggaaagtta ccgtgaaaca 
     4741 gctcaaagaa gattatttca aaaagattga atgtttcgac tctgttgaaa tcagcggagt 
     4801 ggaggatcgc ttcaacgcat ccctgggaac gtatcacgat ctcctgaaaa tcattaaaga 
     4861 caaggacttc ctggacaatg aggagaacga ggacattctt gaggacattg tcctcaccct 
     4921 tacgttgttt gaagataggg agatgattga agaacgcttg aaaacttacg ctcatctctt 
     4981 cgacgacaaa gtcatgaaac agctcaagag gcgccgatat acaggatggg ggcggctgtc 
     5041 aagaaaactg atcaatggga tccgagacaa gcagagtgga aagacaatcc tggattttct 
     5101 taagtccgat ggatttgcca accggaactt catgcagttg atccatgatg actctctcac 
     5161 ctttaaggag gacatccaga aagcacaagt ttctggccag ggggacagtc tccacgagca 
     5221 catcgctaat cttgcaggta gcccagctat caaaaaggga atactgcaga ccgttaaggt 
     5281 cgtggatgaa ctcgtcaaag taatgggaag gcataagccc gagaatatcg ttatcgagat 
     5341 ggcccgagag aaccaaacta cccagaaggg acagaagaac agtagggaaa ggatgaagag 
     5401 gattgaagag ggtataaaag aactggggtc ccaaatcctt aaggaacacc cagttgaaaa 
     5461 cacccagctt cagaatgaga agctctacct gtactacctg cagaacggca gggacatgta 
     5521 cgtggatcag gaactggaca tcaatcggct ctccgactac gacgtggatc atatcgtgcc 
     5581 ccagtctttt ctcaaagatg attctattga taataaagtg ttgacaagat ccgataaaaa 
     5641 tagagggaag agtgataacg tcccctcaga agaagttgtc aagaaaatga aaaattattg 
     5701 gcggcagctg ctgaacgcca aactgatcac acaacggaag ttcgataatc tgactaaggc 
     5761 tgaacgaggt ggcctgtctg agttggataa agccggcttc atcaaaaggc agcttgttga 
     5821 gacacgccag atcaccaagc acgtggccca aattctcgat tcacgcatga acaccaagta 
     5881 cgatgaaaat gacaaactga ttcgagaggt gaaagttatt actctgaagt ctaagctggt 
     5941 ttcagatttc agaaaggact ttcagtttta taaggtgaga gagatcaaca attaccacca 
     6001 tgcgcatgat gcctacctga atgcagtggt aggcactgca cttatcaaaa aatatcccaa 
     6061 gcttgaatct gaatttgttt acggagacta taaagtgtac gatgttagga aaatgatcgc 
     6121 aaagtctgag caggaaatag gcaaggccac cgctaagtac ttcttttaca gcaatattat 
     6181 gaattttttc aagaccgaga ttacactggc caatggagag attcggaagc gaccacttat 
     6241 cgaaacaaac ggagaaacag gagaaatcgt gtgggacaag ggtagggatt tcgcgacagt 
     6301 ccggaaggtc ctgtccatgc cgcaggtgaa catcgttaaa aagaccgaag tacagaccgg 
     6361 aggcttctcc aaggaaagta tcctcccgaa aaggaacagc gacaagctga tcgcacgcaa 
     6421 aaaagattgg gaccccaaga aatacggcgg attcgattct cctacagtcg cttacagtgt 
     6481 actggttgtg gccaaagtgg agaaagggaa gtctaaaaaa ctcaaaagcg tcaaggaact 
     6541 gctgggcatc acaatcatgg agcgatcaag cttcgaaaaa aaccccatcg actttctcga 
     6601 ggcgaaagga tataaagagg tcaaaaaaga cctcatcatt aagcttccca agtactctct 
     6661 ctttgagctt gaaaacggcc ggaaacgaat gctcgctagt gcgggcgagc tgcagaaagg 
     6721 taacgagctg gcactgccct ctaaatacgt taatttcttg tatctggcca gccactatga 
     6781 aaagctcaaa ggatctcccg aagataatga gcagaagcag ctgttcgtgg aacaacacaa 
     6841 acactacctt gatgagatca tcgagcaaat aagcgaattc tccaaaagag tgatcctcgc 
     6901 cgacgctaac ctcgataagg tgctttctgc ttacaataag cacagggata agcccatcag 
     6961 ggagcaggca gaaaacatta tccacttgtt tactctgacc aacttgggcg cgcctgcagc 
     7021 cttcaagtac ttcgacacca ccatagacag aaagcggtac acctctacaa aggaggtcct 
     7081 ggacgccaca ctgattcatc agtcaattac ggggctctat gaaacaagaa tcgacctctc 
     7141 tcagctcggt ggagacagca gggctgaccc caagaagaag aggaaggtgt gagctt 
//
