LOCUS GB1079 7192 bp ds-DNA circular 22-JUN-2015 DEFINITION . FEATURES Location/Qualifiers CDS complement(5440..6300) /label="AmpR" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 misc_feature 5581..5581 /label="A>C" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac misc_signal 9..29 /label="SV40 NLS" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 CDS 30..4141 /label="hCas9_D10A_H840A TTCG" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac misc_feature 7102..7121 /label="T7 primer" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 misc_feature 4142..4145 /label="TTCG OH" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e misc_feature 2543..2546 /label="TGCC OH" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac misc_feature 6696..6696 /label="A>C" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 ORIGIN 1 ctcgaatgcc caagaagaag aggaaggtgg acaagaagta ctccattggg ctcgctatcg 61 gcacaaacag cgtcggctgg gccgtcatta cggccgagta caaggtgccg agcaaaaaat 121 tcaaagttct gggcaatacc gatcgccaca gcataaagaa gaacctcatt ggcgccctcc 181 tgttcgactc cggggaaacg gacgaagcca cgcggctcaa aagaacagca cggcgcagat 241 atacccgcag aaagaatcgg atctgctacc tgcaggagat ctttagtaat gagatggcta 301 aggtggatga ctctttcttc cataggctgg aggagtcctt tttggtggag gaggataaaa 361 agcacgagcg ccacccaatc tttggcaata tcgtggacga ggtggcgtac catgaaaagt 421 acccaaccat atatcatctg aggaagaagc ttgtagacag tactgataag gctgacttgc 481 ggttgatcta tctcgcgctg gcgcatatga tcaaatttcg gggacacttc ctcatcgagg 541 gggacctgaa cccagacaac agcgatgtcg acaaactctt tatccaactg gttcagactt 601 acaatcagct tttcgaagag aacccgatca acgcatccgg agttgacgcc aaagcaatcc 661 tgagcgctag gctgtccaaa tcccggcggc tcgaaaacct catcgcacag ctccctgggg 721 agaagaagaa cggcctgttt ggtaatctta tcgccctgtc actcgggctg acccccaact 781 ttaaatctaa cttcgacctg gccgaagatg ccaagcttca actgagcaaa gacacctacg 841 atgatgatct cgacaatctg ctggcccaga tcggcgacca gtacgcagac ctttttttgg 901 cggcaaagaa cctgtcagac gccattctgc tgagtgatat tctgcgagtg aacacggaga 961 tcaccaaagc tccgctgagc gctagtatga tcaagcgcta tgatgagcac caccaagact 1021 tgactttgct gaaggccctt gtcagacagc aactgcctga gaagtacaag gaaattttct 1081 tcgatcagtc taaaaatggc tacgccggat acattgacgg cggagcaagc caggaggaat 1141 tttacaaatt tattaagccc atcttggaaa aaatggacgg caccgaggag ctgctggtaa 1201 agcttaacag agaagatctg ttgcgcaaac agcgcacttt cgacaatgga agcatccccc 1261 accagattca cctgggcgaa ctgcacgcta tcctcaggcg gcaagaggat ttctacccct 1321 ttttgaaaga taacagggaa aagattgaga aaatcctcac atttcggata ccctactatg 1381 taggccccct cgcccgggga aattccagat tcgcgtggat gactcgcaaa tcagaagaga 1441 ctatcactcc ctggaacttc gaggaagtcg tggataaggg ggcctctgcc cagtccttca 1501 tcgaaaggat gactaacttt gataaaaatc tgcctaacga aaaggtgctt cctaaacact 1561 ctctgctgta cgagtacttc acagtttata acgagctcac caaggtcaaa tacgtcacag 1621 aagggatgag aaagccagca ttcctgtctg gagagcagaa gaaagctatc gtggacctcc 1681 tcttcaagac gaaccggaaa gttaccgtga aacagctcaa agaagattat ttcaaaaaga 1741 ttgaatgttt cgactctgtt gaaatcagcg gagtggagga tcgcttcaac gcatccctgg 1801 gaacgtatca cgatctcctg aaaatcatta aagacaagga cttcctggac aatgaggaga 1861 acgaggacat tcttgaggac attgtcctca cccttacgtt gtttgaagat agggagatga 1921 ttgaagaacg cttgaaaact tacgctcatc tcttcgacga caaagtcatg aaacagctca 1981 agaggcgccg atatacagga tgggggcggc tgtcaagaaa actgatcaat gggatccgag 2041 acaagcagag tggaaagaca atcctggatt ttcttaagtc cgatggattt gccaaccgga 2101 acttcatgca gttgatccat gatgactctc tcacctttaa ggaggacatc cagaaagcac 2161 aagtttctgg ccagggggac agtctccacg agcacatcgc taatcttgca ggtagcccag 2221 ctatcaaaaa gggaatactg cagaccgtta aggtcgtgga tgaactcgtc aaagtaatgg 2281 gaaggcataa gcccgagaat atcgttatcg agatggcccg agagaaccaa actacccaga 2341 agggacagaa gaacagtagg gaaaggatga agaggattga agagggtata aaagaactgg 2401 ggtcccaaat ccttaaggaa cacccagttg aaaacaccca gcttcagaat gagaagctct 2461 acctgtacta cctgcagaac ggcagggaca tgtacgtgga tcaggaactg gacatcaatc 2521 ggctctccga ctacgacgtg gatgccatcg tgccccagtc ttttctcaaa gatgattcta 2581 ttgataataa agtgttgaca agatccgata aaaatagagg gaagagtgat aacgtcccct 2641 cagaagaagt tgtcaagaaa atgaaaaatt attggcggca gctgctgaac gccaaactga 2701 tcacacaacg gaagttcgat aatctgacta aggctgaacg aggtggcctg tctgagttgg 2761 ataaagccgg cttcatcaaa aggcagcttg ttgagacacg ccagatcacc aagcacgtgg 2821 cccaaattct cgattcacgc atgaacacca agtacgatga aaatgacaaa ctgattcgag 2881 aggtgaaagt tattactctg aagtctaagc tggtttcaga tttcagaaag gactttcagt 2941 tttataaggt gagagagatc aacaattacc accatgcgca tgatgcctac ctgaatgcag 3001 tggtaggcac tgcacttatc aaaaaatatc ccaagcttga atctgaattt gtttacggag 3061 actataaagt gtacgatgtt aggaaaatga tcgcaaagtc tgagcaggaa ataggcaagg 3121 ccaccgctaa gtacttcttt tacagcaata ttatgaattt tttcaagacc gagattacac 3181 tggccaatgg agagattcgg aagcgaccac ttatcgaaac aaacggagaa acaggagaaa 3241 tcgtgtggga caagggtagg gatttcgcga cagtccggaa ggtcctgtcc atgccgcagg 3301 tgaacatcgt taaaaagacc gaagtacaga ccggaggctt ctccaaggaa agtatcctcc 3361 cgaaaaggaa cagcgacaag ctgatcgcac gcaaaaaaga ttgggacccc aagaaatacg 3421 gcggattcga ttctcctaca gtcgcttaca gtgtactggt tgtggccaaa gtggagaaag 3481 ggaagtctaa aaaactcaaa agcgtcaagg aactgctggg catcacaatc atggagcgat 3541 caagcttcga aaaaaacccc atcgactttc tcgaggcgaa aggatataaa gaggtcaaaa 3601 aagacctcat cattaagctt cccaagtact ctctctttga gcttgaaaac ggccggaaac 3661 gaatgctcgc tagtgcgggc gagctgcaga aaggtaacga gctggcactg ccctctaaat 3721 acgttaattt cttgtatctg gccagccact atgaaaagct caaaggatct cccgaagata 3781 atgagcagaa gcagctgttc gtggaacaac acaaacacta ccttgatgag atcatcgagc 3841 aaataagcga attctccaaa agagtgatcc tcgccgacgc taacctcgat aaggtgcttt 3901 ctgcttacaa taagcacagg gataagccca tcagggagca ggcagaaaac attatccact 3961 tgtttactct gaccaacttg ggcgcgcctg cagccttcaa gtacttcgac accaccatag 4021 acagaaagcg gtacacctct acaaaggagg tcctggacgc cacactgatt catcagtcaa 4081 ttacggggct ctatgaaaca agaatcgacc tctctcagct cggtggagac agcagggctg 4141 attcgcgaga ccactcatcg cccatcacta gtgaattcgc ggccgcctgc aggtcgacca 4201 tatgggagag ctcccaacgc gttggatgca tagcttgagt attctatagt gtcacctaaa 4261 tagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat 4321 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag 4381 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg 4441 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc 4501 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 4561 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 4621 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 4681 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 4741 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 4801 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 4861 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 4921 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 4981 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 5041 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 5101 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac 5161 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 5221 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 5281 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 5341 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 5401 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 5461 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 5521 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 5581 cgacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 5641 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 5701 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 5761 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 5821 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 5881 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 5941 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 6001 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 6061 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 6121 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 6181 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 6241 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 6301 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 6361 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 6421 agtgccacct gatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca 6481 ggaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 6541 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 6601 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 6661 caacgtcaaa gggcgaaaaa ccgtctatca gggcgctggc ccactacgtg aaccatcacc 6721 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 6781 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 6841 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 6901 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc attcgccatt caggctgcgc 6961 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg 7021 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt 7081 aaaacgacgg ccagtgaatt gtaatacgac tcactatagg gcgaattggg cccgacgtcg 7141 catgctcccg gccgccatgg cggccgcggg aattcgatgg gcgatgagtg gt //