LOCUS       GB1182        3779 bp    DNA     circular     17-JUN-2015
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|727624968|
COMMENT     VNTDBDATE|736960539|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pUPD: GFP localizacao C|
COMMENT     VNTAUTHORNAME|Silvia Presa|
COMMENT     VNTAUTHOREML|silviapr@upvnet.upv.es|
FEATURES             Location/Qualifiers
     CDS             complement(1295..2155)
                     /vntifkey="4"
                     /label=AmpR
     mutation        1436..1436
                     /vntifkey="62"
                     /label=A>C\(mutar\BsaI)
     misc_feature    1435..1438
                     /vntifkey="21"
                     /label=OVERHANG
     misc_feature    2550..2553
                     /vntifkey="21"
                     /label=OVERHANG
     mutation        2551..2551
                     /vntifkey="62"
                     /label=A>C\(mutar\BtgZI)
     misc_feature    3052..3775
                     /vntifkey="21"
                     /label=GFP
BASE COUNT      951 a      1002 c       984 g       842 t 
ORIGIN
        1 cgagaccact catcgcccat cactagtgaa ttcgcggccg cctgcaggtc gaccatatgg 
       61 gagagctccc aacgcgttgg atgcatagct tgagtattct atagtgtcac ctaaatagct 
      121 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac 
      181 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac 
      241 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc 
      301 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg 
      361 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 
      421 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 
      481 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 
      541 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 
      601 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 
      661 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 
      721 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 
      781 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 
      841 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 
      901 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 
      961 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 
     1021 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 
     1081 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 
     1141 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 
     1201 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 
     1261 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 
     1321 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 
     1381 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgcgacc 
     1441 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 
     1501 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 
     1561 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 
     1621 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 
     1681 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 
     1741 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 
     1801 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 
     1861 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 
     1921 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 
     1981 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 
     2041 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 
     2101 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 
     2161 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 
     2221 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 
     2281 cacctgatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggaaa 
     2341 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt 
     2401 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag 
     2461 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg 
     2521 tcaaagggcg aaaaaccgtc tatcagggcg ctggcccact acgtgaacca tcaccctaat 
     2581 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc 
     2641 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga 
     2701 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac 
     2761 ccgccgcgct taatgcgccg ctacagggcg cgtccattcg ccattcaggc tgcgcaactg 
     2821 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg 
     2881 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac 
     2941 gacggccagt gaattgtaat acgactcact atagggcgaa ttgggcccga cgtcgcatgc 
     3001 tcccggccgc catggcggcc gcgggaattc gatgggcgat gagtggtctc gttcgatggt 
     3061 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga 
     3121 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgacgcca cctacggcaa 
     3181 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt 
     3241 gaccaccttc agctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca 
     3301 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa 
     3361 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa 
     3421 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct 
     3481 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat 
     3541 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca 
     3601 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct 
     3661 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct 
     3721 ggagttcgtg accgccgccg ggatcactca cggcatggac gagctgtaca agtgagctt 
//
