LOCUS       GB1191              12041 bp    DNA              UNK 01-JAN-1980
DEFINITION  (GB0030,GB01041,GB0037)pDGB3_alpha2
ACCESSION   GB1191
VERSION     GB1191
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        5..1031
                     /vntifkey="30"
                     /label="35S"
     misc_feature    5149..5169
                     /ApEinfo_fwdcolor="#9eafd2"
                     /label="NLS"
                     /ApEinfo_revcolor="#9eafd2"
     CDS             1033..5169
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /label="mCas9"
                     /ApEinfo_revcolor="#ff9ccd"
     mutation        5257
                     /vntifkey="62"
                     /label="T>C"
     terminator      5173..5668
                     /vntifkey="43"
                     /label="Tnos"
     misc_feature    5686..5710
                     /vntifkey="21"
                     /label="RB"
     misc_feature    11995..12020
                     /vntifkey="21"
                     /label="LB"
     CDS             complement(10750..11562)
                     /note="kanamycin resistance gene"
                     /vntifkey="4"
                     /label="KanR"
     rep_origin      complement(10175..10455)
                     /note="pBR322 origin of replication"
                     /vntifkey="33"
                     /label="pBR322\ori"
     misc_feature    complement(9775..10035)
                     /note="bom site from pBR322"
                     /vntifkey="21"
                     /label="pBR322"
     misc_feature    complement(6772..7772)
                     /note="STA region from pVS1 plasmid"
                     /vntifkey="21"
                     /label="STA\region\from\pVS1\plasmid"
     misc_feature    complement(8365..9365)
                     /note="pVS1-REP; replication origin from pVS1"
                     /vntifkey="21"
                     /label="pVS1-Rep\ori"
ORIGIN
        1 ggagactaga gccaagctga tctcctttgc cccggagatc accatggacg actttctcta
       61 tctctacgat ctaggaagaa agttcgacgg agaaggtgac gataccatgt tcaccaccga
      121 taatgagaag attagcctct tcaatttcag aaagaatgct gacccacaga tggttagaga
      181 ggcctacgcg gcaggtctga tcaagacgat ctacccgagt aataatctcc aggagatcaa
      241 ataccttccc aagaaggtta aagatgcagt caaaagattc aggactaact gcatcaagaa
      301 cacagagaaa gatatatttc tcaagatcag aagtactatt ccagtatgga cgattcaagg
      361 cttgcttcat aaaccaaggc aagtaataga gattggagtc tctaagaaag tagttcctac
      421 tgaatcaaag gccatggagt caaaaattca gatcgaggat ctaacagaac tcgccgtgaa
      481 gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga aaatcttcgt
      541 caacatggtg gagcacgaca ctctcgtcta ctccaagaat atcaaagata cagtctcaga
      601 agaccaaagg gctattgaga cttttcaaca aagggtaata tcgggaaacc tcctcggatt
      661 ccattgccca gctatctgtc acttcatcaa aaggacagta gaaaaggaag gtggcaccta
      721 caaatgccat cattgcgata aaggaaaggc tatcgttcaa gatgcctctg ccgacagtgg
      781 tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac
      841 gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg acgcacaatc
      901 ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac
      961 tccggtattt ttacaacaat accacaacaa aacaaacaac aaacaacatt acaatttact
     1021 attctagtcg aaatggacaa gaagtactcc attgggctcg ctatcggcac aaacagcgtc
     1081 ggctgggccg tcattacggc cgagtacaag gtgccgagca aaaaattcaa agttctgggc
     1141 aataccgatc gccacagcat aaagaagaac ctcattggcg ccctcctgtt cgactccggg
     1201 gaaacggacg aagccacgcg gctcaaaaga acagcacggc gcagatatac ccgcagaaag
     1261 aatcggatct gctacctgca ggagatcttt agtaatgaga tggctaaggt ggatgactct
     1321 ttcttccata ggctggagga gtcctttttg gtggaggagg ataaaaagca cgagcgccac
     1381 ccaatctttg gcaatatcgt ggacgaggtg gcgtaccatg aaaagtaccc aaccatatat
     1441 catctgagga agaagcttgt agacagtact gataaggctg acttgcggtt gatctatctc
     1501 gcgctggcgc atatgatcaa atttcgggga cacttcctca tcgaggggga cctgaaccca
     1561 gacaacagcg atgtcgacaa actctttatc caactggttc agacttacaa tcagcttttc
     1621 gaagagaacc cgatcaacgc atccggagtt gacgccaaag caatcctgag cgctaggctg
     1681 tccaaatccc ggcggctcga aaacctcatc gcacagctcc ctggggagaa gaagaacggc
     1741 ctgtttggta atcttatcgc cctgtcactc gggctgaccc ccaactttaa atctaacttc
     1801 gacctggccg aagatgccaa gcttcaactg agcaaagaca cctacgatga tgatctcgac
     1861 aatctgctgg cccagatcgg cgaccagtac gcagaccttt ttttggcggc aaagaacctg
     1921 tcagacgcca ttctgctgag tgatattctg cgagtgaaca cggagatcac caaagctccg
     1981 ctgagcgcta gtatgatcaa gcgctatgat gagcaccacc aagacttgac tttgctgaag
     2041 gcccttgtca gacagcaact gcctgagaag tacaaggaaa ttttcttcga tcagtctaaa
     2101 aatggctacg ccggatacat tgacggcgga gcaagccagg aggaatttta caaatttatt
     2161 aagcccatct tggaaaaaat ggacggcacc gaggagctgc tggtaaagct taacagagaa
     2221 gatctgttgc gcaaacagcg cactttcgac aatggaagca tcccccacca gattcacctg
     2281 ggcgaactgc acgctatcct caggcggcaa gaggatttct accccttttt gaaagataac
     2341 agggaaaaga ttgagaaaat cctcacattt cggataccct actatgtagg ccccctcgcc
     2401 cggggaaatt ccagattcgc gtggatgact cgcaaatcag aagagactat cactccctgg
     2461 aacttcgagg aagtcgtgga taagggggcc tctgcccagt ccttcatcga aaggatgact
     2521 aactttgata aaaatctgcc taacgaaaag gtgcttccta aacactctct gctgtacgag
     2581 tacttcacag tttataacga gctcaccaag gtcaaatacg tcacagaagg gatgagaaag
     2641 ccagcattcc tgtctggaga gcagaagaaa gctatcgtgg acctcctctt caagacgaac
     2701 cggaaagtta ccgtgaaaca gctcaaagaa gattatttca aaaagattga atgtttcgac
     2761 tctgttgaaa tcagcggagt ggaggatcgc ttcaacgcat ccctgggaac gtatcacgat
     2821 ctcctgaaaa tcattaaaga caaggacttc ctggacaatg aggagaacga ggacattctt
     2881 gaggacattg tcctcaccct tacgttgttt gaagataggg agatgattga agaacgcttg
     2941 aaaacttacg ctcatctctt cgacgacaaa gtcatgaaac agctcaagag gcgccgatat
     3001 acaggatggg ggcggctgtc aagaaaactg atcaatggga tccgagacaa gcagagtgga
     3061 aagacaatcc tggattttct taagtccgat ggatttgcca accggaactt catgcagttg
     3121 atccatgatg actctctcac ctttaaggag gacatccaga aagcacaagt ttctggccag
     3181 ggggacagtc tccacgagca catcgctaat cttgcaggta gcccagctat caaaaaggga
     3241 atactgcaga ccgttaaggt cgtggatgaa ctcgtcaaag taatgggaag gcataagccc
     3301 gagaatatcg ttatcgagat ggcccgagag aaccaaacta cccagaaggg acagaagaac
     3361 agtagggaaa ggatgaagag gattgaagag ggtataaaag aactggggtc ccaaatcctt
     3421 aaggaacacc cagttgaaaa cacccagctt cagaatgaga agctctacct gtactacctg
     3481 cagaacggca gggacatgta cgtggatcag gaactggaca tcaatcggct ctccgactac
     3541 gacgtggatg ccatcgtgcc ccagtctttt ctcaaagatg attctattga taataaagtg
     3601 ttgacaagat ccgataaaaa tagagggaag agtgataacg tcccctcaga agaagttgtc
     3661 aagaaaatga aaaattattg gcggcagctg ctgaacgcca aactgatcac acaacggaag
     3721 ttcgataatc tgactaaggc tgaacgaggt ggcctgtctg agttggataa agccggcttc
     3781 atcaaaaggc agcttgttga gacacgccag atcaccaagc acgtggccca aattctcgat
     3841 tcacgcatga acaccaagta cgatgaaaat gacaaactga ttcgagaggt gaaagttatt
     3901 actctgaagt ctaagctggt ttcagatttc agaaaggact ttcagtttta taaggtgaga
     3961 gagatcaaca attaccacca tgcgcatgat gcctacctga atgcagtggt aggcactgca
     4021 cttatcaaaa aatatcccaa gcttgaatct gaatttgttt acggagacta taaagtgtac
     4081 gatgttagga aaatgatcgc aaagtctgag caggaaatag gcaaggccac cgctaagtac
     4141 ttcttttaca gcaatattat gaattttttc aagaccgaga ttacactggc caatggagag
     4201 attcggaagc gaccacttat cgaaacaaac ggagaaacag gagaaatcgt gtgggacaag
     4261 ggtagggatt tcgcgacagt ccggaaggtc ctgtccatgc cgcaggtgaa catcgttaaa
     4321 aagaccgaag tacagaccgg aggcttctcc aaggaaagta tcctcccgaa aaggaacagc
     4381 gacaagctga tcgcacgcaa aaaagattgg gaccccaaga aatacggcgg attcgattct
     4441 cctacagtcg cttacagtgt actggttgtg gccaaagtgg agaaagggaa gtctaaaaaa
     4501 ctcaaaagcg tcaaggaact gctgggcatc acaatcatgg agcgatcaag cttcgaaaaa
     4561 aaccccatcg actttctcga ggcgaaagga tataaagagg tcaaaaaaga cctcatcatt
     4621 aagcttccca agtactctct ctttgagctt gaaaacggcc ggaaacgaat gctcgctagt
     4681 gcgggcgagc tgcagaaagg taacgagctg gcactgccct ctaaatacgt taatttcttg
     4741 tatctggcca gccactatga aaagctcaaa ggatctcccg aagataatga gcagaagcag
     4801 ctgttcgtgg aacaacacaa acactacctt gatgagatca tcgagcaaat aagcgaattc
     4861 tccaaaagag tgatcctcgc cgacgctaac ctcgataagg tgctttctgc ttacaataag
     4921 cacagggata agcccatcag ggagcaggca gaaaacatta tccacttgtt tactctgacc
     4981 aacttgggcg cgcctgcagc cttcaagtac ttcgacacca ccatagacag aaagcggtac
     5041 acctctacaa aggaggtcct ggacgccaca ctgattcatc agtcaattac ggggctctat
     5101 gaaacaagaa tcgacctctc tcagctcggt ggagacagca gggctgaccc caagaagaag
     5161 aggaaggtgt gagcttggaa tggatcttcg atcccgatcg ttcaaacatt tggcaataaa
     5221 gtttcttaag attgaatcct gttgccggtc ttgcgacgat tatcatataa tttctgttga
     5281 attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt
     5341 ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg
     5401 caaactagga taaattatcg cgcdcggtgt catctatgtt actagatcgg gaattgccaa
     5461 gctaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     5521 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     5581 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     5641 taaatgcttc aataatggga ccgactcgcg cttgagacga agctttgaca ggatatattg
     5701 gcgggtaaac ctaagagaaa agagcgttta ttagaataat cggatattta aaagggcgtg
     5761 aaaaggttta tccgttcgtc catttgtatg tgcatgccaa ccacagggtt cccctcggga
     5821 tcaaagtact ttgatccaac ccctccgctg ctatagtgca gtcggcttct gacgttcagt
     5881 gcagccgtca tctgaaaacg acatgtcgca caagtcctaa gttacgcgac aggctgccgc
     5941 cctgcccttt tcctggcgtt ttcttgtcgc gtgttttagt cgcataaagt agaatacttg
     6001 cgactagaac cggagacatt acgccatgaa caagagcgcc gccgctggcc tgctgggcta
     6061 tgcccgcgtc agcaccgacg accaggactt gaccaaccaa cgggccgaac tgcacgcggc
     6121 cggctgcacc aagctgtttt ccgagaagat caccggcacc aggcgcgacc gcccggagct
     6181 ggccaggatg cttgaccacc tacgccctgg cgacgttgtg acagtgacca ggctagaccg
     6241 cctggcccgc agcacccgcg acctactgga cattgccgag cgcatccagg aggccggcgc
     6301 gggcctgcgt agcctggcag agccgtgggc cgacaccacc acgccggccg gccgcatggt
     6361 gttgaccgtg ttcgccggca ttgccgagtt cgagcgttcc ctaatcatcg accgcacccg
     6421 gagcgggcgc gaggccgcca aggcccgagg cgtgaagttt ggcccccgcc ctaccctcac
     6481 cccggcacag atcgcgcacg cccgcgagct gatcgaccag gaaggccgca ccgtgaaaga
     6541 ggcggctgca ctgcttggcg tgcatcgctc gaccctgtac cgcgcacttg agcgcagcga
     6601 ggaagtgacg cccaccgagg ccaggcggcg cggtgccttc cgtgaggacg cattgaccga
     6661 ggccgacgcc ctggcggccg ccgagaatga acgccaagag gaacaagcat gaaaccgcac
     6721 caggacggcc aggacgaacc gtttttcatt accgaagaga tcgaggcgga gatgatcgcg
     6781 gccgggtacg tgttcgagcc gcccgcgcac ctctcaaccg tgcggctgca tgaaatcctg
     6841 gccggtttgt ctgatgccaa gctggcggcc tggccggcca gcttggccgc tgaagaaacc
     6901 gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa acagcttgcg tcatgcggtc
     6961 gctgcgtata tgatccgatg agtaaataaa caaatacgca aggggaacgc atgaaggtta
     7021 tcgctgtact taaccagaaa ggcgggtcag gcaagacgac catcggaacc catctagccc
     7081 gcgccctgca actcgccggg gccgatgttc tgttagtcga ttccgatccc cagggcagtg
     7141 cccgcgattg ggcggccgtg cgggaagatc aaccgctaac cgttgtcggc atcgaccgcc
     7201 cgacgattga ccgcgacgtg aaggccatcg gccggcgcga cttcgtagtg atcgacggag
     7261 cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc agccgacttc gtgctgattc
     7321 cggtgcagcc aagcccttac gacatatggg ccaccgccga cctggtggag ctggttaagc
     7381 agcgcattga ggtcacggat ggaaggctac aagcggcctt tgtcgtgtcg cgggcgatca
     7441 aaggcacgcg catcggcggt gaggttgccg aggcgctggc cgggtacgag ctgcccattc
     7501 ttgagtcccg tatcacgcag cgcgtgagct acccaggcac tgccgccgcc ggcacaaccg
     7561 ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt ccaggcgctg gccgctgaaa
     7621 ttaaatcaaa actcatttga gttaatgagg taaagagaaa atgagcaaaa gcacaaacac
     7681 gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct gcaacgttgg ccagcctggc
     7741 agacacgcca gccatgaagc gggtcaactt tcagttgccg gcggaggatc acaccaagct
     7801 gaagatgtac gcggtacgcc aaggcaagac cattaccgag ctgctatctg aatagatcgc
     7861 gcagctacca gagtaaatga gcaaatgaat aaatgagtag atgaatttta gcggctaaag
     7921 gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc cgtggaatgc cccatgtgtg
     7981 gaggaacggg cggttggcca ggcgtaagcg gctgggttgt ctgccggccc tgcaatggca
     8041 ctggaacccc caagcccgag gaatcggcgt gacggtcgca aaccatccgg cccggtacaa
     8101 atcggcgcgg cgctgggtga tgacctggtg gagaagttga aggccgcgca ggccgcccag
     8161 cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga
     8221 atccgcaaag aatcccggca accgccggca gccggtgcgc cgtcgattag gaagccgccc
     8281 aagggcgacg agcaaccaga ttttttcgtt ccgatgctct atgacgtggg cacccgcgat
     8341 agtcgcagca tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg acgagctggc
     8401 gaggtgatcc gctacgagct tccagacggg cacgtagagg tttccgcagg gccggccggc
     8461 atggccagtg tgtgggatta cgacctggta ctgatggcgg tttcccatct aaccgaatcc
     8521 atgaaccgat accgggaagg gaagggagac aagcccggcc gcgtgttccg tccacacgtt
     8581 gcggacgtac tcaagttctg ccggcgagcc gatggcggaa agcagaaaga cgacctggta
     8641 gaaacctgca ttcggttaaa caccacgcac gttgccatgc agcgtacgaa gaaggccaag
     8701 aacggccgcc tggtgacggt atccgagggt gaagccttga ttagccgcta caagatcgta
     8761 aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc tagctgattg gatgtaccgc
     8821 gagatcacag aaggcaagaa cccggacgtg ctgacggttc accccgatta ctttttgatc
     8881 gatcccggca tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa
     8941 gccagatggt tgttcaagac gatctacgaa cgcagtggca gcgccggaga gttcaagaag
     9001 ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta cgatttgaag
     9061 gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct accgcaacct gatcgagggc
     9121 gaagcatccg ccggttccta atgtacggag cagatgctag ggcaaattgc cctagcaggg
     9181 gaaaaaggtc gaaaaggact ctttcctgtg gatagcacgt acattgggaa cccaaagccg
     9241 tacattggga accggaaccc gtacattggg aacccaaagc cgtacattgg gaaccggtca
     9301 cacatgtaag tgactgatat aaaagagaaa aaaggcgatt tttccgccta aaactcttta
     9361 aaacttatta aaactcttaa aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca
     9421 gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg ccccgccgct
     9481 tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat
     9541 ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc
     9601 accctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccggt
     9661 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     9721 agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt
     9781 gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
     9841 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc
     9901 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     9961 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
    10021 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
    10081 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
    10141 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
    10201 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
    10261 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
    10321 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
    10381 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
    10441 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
    10501 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
    10561 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
    10621 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
    10681 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgcattct
    10741 aggtgattag aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt
    10801 atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca
    10861 gttccatagg atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat
    10921 acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt
    10981 gacgactgaa tccggtgaga atggcaaaag tttatgcatt tctttccaga cttgttcaac
    11041 aggccagcca ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg
    11101 tgattgcgcc tgagcgagtc gaaatacgcg atcgctgtta aaaggacaat tacaaacagg
    11161 aatcgaatgc aaccggcgca ggaacactgc cagcgcatca acaatatttt cacctgaatc
    11221 aggatattct tctaatacct ggaatgctgt tttccctggg atcgcagtgg tgagtaacca
    11281 tgcatcatca ggagtacgga taaaatgctt gatggtcgga agaggcataa attccgtcag
    11341 ccagtttagt ctgaccatct catctgtaac atcattggca acgctacctt tgccatgttt
    11401 cagaaacaac tctggcgcat cgggcttccc atacaatcgg tagattgtcg cacctgattg
    11461 cccgacatta tcgcgagccc atttataccc atataaatca gcatccatgt tggaatttaa
    11521 tcgcggcctt gagcaagacg tttcccgttg aatatggctc ataacagaac ttattatttc
    11581 cttcctcttt tctacagtat ttaaagatac cccaagaagc taattataac aagacgaact
    11641 ccaattcact gttccttgca ttctaaaacc ttaaatacca gaaaacagct ttttcaaagt
    11701 tgttttcaaa gttggcgtat aacatagtat cgacggagcc gattttgaaa ccgcggtgat
    11761 cacaggcagc aacgctctgt catcgttaca atcaacatgc taccctccgc gagatcatcc
    11821 gtgtttcaaa cccggcagct tagttgccgt tcttccgaat agcatcggta acatgagcaa
    11881 agtctgccgc cttacaacgg ctctcccgct gacgccgtcc cggactgatg ggctgcctgt
    11941 atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg ctggtggcag
    12001 gatatattgt ggtgtaaaca taacaagctt cgtctcagtc a
//
