LOCUS       GB1403                  8520 bp    DNA              UNK 01-JAN-1980
DEFINITION  (GB0030,GB1435,GB1186,GB0037)pDGB3_alpha1
ACCESSION   GB1403
VERSION     GB1403
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        5..1031
                     /vntifkey="30"
                     /label="35S"
     misc_feature    1477..1500
                     /ApEinfo_fwdcolor="#b1ff67"
                     /label="NLS"
                     /ApEinfo_revcolor="#b1ff67"
     misc_feature    1453..1476
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /label="NLS"
                     /ApEinfo_revcolor="#ff9ccd"
     misc_feature    1501..1650
                     /ApEinfo_fwdcolor="#85dae9"
                     /label="VP64"
                     /ApEinfo_revcolor="#85dae9"
     misc_feature    1429..1452
                     /ApEinfo_fwdcolor="#85dae9"
                     /label="NLS"
                     /ApEinfo_revcolor="#85dae9"
     mutation        1738
                     /vntifkey="62"
                     /label="T>C"
     terminator      1654..2149
                     /vntifkey="43"
                     /label="Tnos"
     misc_feature    2154..2157
                     /vntifkey="21"
                     /label="GTCA"
     misc_feature    2150..2153
                     /vntifkey="21"
                     /label="CGCT"
     misc_feature    2170..2194
                     /vntifkey="21"
                     /label="RB"
     misc_feature    8479..8504
                     /vntifkey="21"
                     /label="LB"
     CDS             complement(7234..8046)
                     /note="kanamycin resistance gene"
                     /vntifkey="4"
                     /label="KanR"
     rep_origin      complement(6659..6939)
                     /note="pBR322 origin of replication"
                     /vntifkey="33"
                     /label="pBR322\ori"
     misc_feature    complement(6259..6519)
                     /note="bom site from pBR322"
                     /vntifkey="21"
                     /label="pBR322"
     misc_feature    complement(3256..4256)
                     /note="STA region from pVS1 plasmid"
                     /vntifkey="21"
                     /label="STA\region\from\pVS1\plasmid"
     misc_feature    complement(4849..5849)
                     /note="pVS1-REP; replication origin from pVS1"
                     /vntifkey="21"
                     /label="pVS1-Rep\ori"
ORIGIN
        1 ggagactaga gccaagctga tctcctttgc cccggagatc accatggacg actttctcta
       61 tctctacgat ctaggaagaa agttcgacgg agaaggtgac gataccatgt tcaccaccga
      121 taatgagaag attagcctct tcaatttcag aaagaatgct gacccacaga tggttagaga
      181 ggcctacgcg gcaggtctga tcaagacgat ctacccgagt aataatctcc aggagatcaa
      241 ataccttccc aagaaggtta aagatgcagt caaaagattc aggactaact gcatcaagaa
      301 cacagagaaa gatatatttc tcaagatcag aagtactatt ccagtatgga cgattcaagg
      361 cttgcttcat aaaccaaggc aagtaataga gattggagtc tctaagaaag tagttcctac
      421 tgaatcaaag gccatggagt caaaaattca gatcgaggat ctaacagaac tcgccgtgaa
      481 gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga aaatcttcgt
      541 caacatggtg gagcacgaca ctctcgtcta ctccaagaat atcaaagata cagtctcaga
      601 agaccaaagg gctattgaga cttttcaaca aagggtaata tcgggaaacc tcctcggatt
      661 ccattgccca gctatctgtc acttcatcaa aaggacagta gaaaaggaag gtggcaccta
      721 caaatgccat cattgcgata aaggaaaggc tatcgttcaa gatgcctctg ccgacagtgg
      781 tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac
      841 gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg acgcacaatc
      901 ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac
      961 tccggtattt ttacaacaat accacaacaa aacaaacaac aaacaacatt acaatttact
     1021 attctagtcg aaatgatggc ttctaatttt actcaatttg ttcttgttga taatggtggt
     1081 actggtgatg ttactgttgc tccttctaat tttgctaatg gtgttgctga atggatttct
     1141 tctaattcta gatctcaagc ttataaggtt acttgttctg ttagacaatc ttctgctcaa
     1201 aatagaaagt atactattaa ggttgaagtt cctaaggttg ctactcaaac tgttggtggt
     1261 gttgaacttc ctgttgctgc ttggagatct tatcttaata tggaacttac tattcctatt
     1321 tttgctacta attctgattg tgaacttatt gttaaggcta tgcaaggtct tcttaaggat
     1381 ggtaatccta ttccttctgc tattgctgct aattctggta tttattcgga cccaaaaaag
     1441 aagcgtaagg tcgatcccaa gaagaagaga aaggtagatc ctaagaagaa gagaaaggta
     1501 gacgcattgg atgacttcga cttagacatg cttgggtcag atgctttgga cgactttgac
     1561 ctcgacatgt tgggttctga cgcgctagat gacttcgact tggacatgtt gggctccgac
     1621 gctttggacg actttgatct ggacatgtta tgagcttgga atggatcttc gatcccgatc
     1681 gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgacga
     1741 ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga
     1801 cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga
     1861 tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcdcggtg tcatctatgt
     1921 tactagatcg ggaattgcca agctaattct tgaagacgaa agggcctcgt gatacgccta
     1981 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     2041 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     2101 ctcatgagac aataaccctg ataaatgctt caataatggg accgactcgc gctgtcatga
     2161 gacgaattct gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa
     2221 taatcggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg
     2281 ccaaccacag ggttcccctc gggatcaaag tactttgatc caacccctcc gctgctatag
     2341 tgcagtcggc ttctgacgtt cagtgcagcc gtcatctgaa aacgacatgt cgcacaagtc
     2401 ctaagttacg cgacaggctg ccgccctgcc cttttcctgg cgttttcttg tcgcgtgttt
     2461 tagtcgcata aagtagaata cttgcgacta gaaccggaga cattacgcca tgaacaagag
     2521 cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg acttgaccaa
     2581 ccaacgggcc gaactgcacg cggccggctg caccaagctg ttttccgaga agatcaccgg
     2641 caccaggcgc gaccgcccgg agctggccag gatgcttgac cacctacgcc ctggcgacgt
     2701 tgtgacagtg accaggctag accgcctggc ccgcagcacc cgcgacctac tggacattgc
     2761 cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg gcagagccgt gggccgacac
     2821 caccacgccg gccggccgca tggtgttgac cgtgttcgcc ggcattgccg agttcgagcg
     2881 ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc gccaaggccc gaggcgtgaa
     2941 gtttggcccc cgccctaccc tcaccccggc acagatcgcg cacgcccgcg agctgatcga
     3001 ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc gctcgaccct
     3061 gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc gaggccaggc ggcgcggtgc
     3121 cttccgtgag gacgcattga ccgaggccga cgccctggcg gccgccgaga atgaacgcca
     3181 agaggaacaa gcatgaaacc gcaccaggac ggccaggacg aaccgttttt cattaccgaa
     3241 gagatcgagg cggagatgat cgcggccggg tacgtgttcg agccgcccgc gcacctctca
     3301 accgtgcggc tgcatgaaat cctggccggt ttgtctgatg ccaagctggc ggcctggccg
     3361 gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg tgtatttgag
     3421 taaaacagct tgcgtcatgc ggtcgctgcg tatatgatcc gatgagtaaa taaacaaata
     3481 cgcaagggga acgcatgaag gttatcgctg tacttaacca gaaaggcggg tcaggcaaga
     3541 cgaccatcgg aacccatcta gcccgcgccc tgcaactcgc cggggccgat gttctgttag
     3601 tcgattccga tccccagggc agtgcccgcg attgggcggc cgtgcgggaa gatcaaccgc
     3661 taaccgttgt cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc atcggccggc
     3721 gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga cttggctgtg tccgcgatca
     3781 aggcagccga cttcgtgctg attccggtgc agccaagccc ttacgacata tgggccaccg
     3841 ccgacctggt ggagctggtt aagcagcgca ttgaggtcac ggatggaagg ctacaagcgg
     3901 cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt gccgaggcgc
     3961 tggccgggta cgagctgccc attcttgagt cccgtatcac gcagcgcgtg agctacccag
     4021 gcactgccgc cgccggcaca accgttcttg aatcagaacc cgagggcgac gctgcccgcg
     4081 aggtccaggc gctggccgct gaaattaaat caaaactcat ttgagttaat gaggtaaaga
     4141 gaaaatgagc aaaagcacaa acacgctaag tgccggccgt ccgagcgcac gcagcagcaa
     4201 ggctgcaacg ttggccagcc tggcagacac gccagccatg aagcgggtca actttcagtt
     4261 gccggcggag gatcacacca agctgaagat gtacgcggta cgccaaggca agaccattac
     4321 cgagctgcta tctgaataga tcgcgcagct accagagtaa atgagcaaat gaataaatga
     4381 gtagatgaat tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa ccaggcaccg
     4441 acgccgtgga atgccccatg tgtggaggaa cgggcggttg gccaggcgta agcggctggg
     4501 ttgtctgccg gccctgcaat ggcactggaa cccccaagcc cgaggaatcg gcgtgacggt
     4561 cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg gtgatgacct ggtggagaag
     4621 ttgaaggccg cgcaggccgc ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa
     4681 tcgtggcaag cggccgctga tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt
     4741 gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac cagatttttt cgttccgatg
     4801 ctctatgacg tgggcacccg cgatagtcgc agcatcatgg acgtggccgt tttccgtctg
     4861 tcgaagcgtg accgacgagc tggcgaggtg atccgctacg agcttccaga cgggcacgta
     4921 gaggtttccg cagggccggc cggcatggcc agtgtgtggg attacgacct ggtactgatg
     4981 gcggtttccc atctaaccga atccatgaac cgataccggg aagggaaggg agacaagccc
     5041 ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt tctgccggcg agccgatggc
     5101 ggaaagcaga aagacgacct ggtagaaacc tgcattcggt taaacaccac gcacgttgcc
     5161 atgcagcgta cgaagaaggc caagaacggc cgcctggtga cggtatccga gggtgaagcc
     5221 ttgattagcc gctacaagat cgtaaagagc gaaaccgggc ggccggagta catcgagatc
     5281 gagctagctg attggatgta ccgcgagatc acagaaggca agaacccgga cgtgctgacg
     5341 gttcaccccg attacttttt gatcgatccc ggcatcggcc gttttctcta ccgcctggca
     5401 cgccgcgccg caggcaaggc agaagccaga tggttgttca agacgatcta cgaacgcagt
     5461 ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat
     5521 gacctgccgg agtacgattt gaaggaggag gcggggcagg ctggcccgat cctagtcatg
     5581 cgctaccgca acctgatcga gggcgaagca tccgccggtt cctaatgtac ggagcagatg
     5641 ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag gactctttcc tgtggatagc
     5701 acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat tgggaaccca
     5761 aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga gaaaaaaggc
     5821 gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg cctggcctgt
     5881 gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac ccttcggtcg
     5941 ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg ccgctcaaaa
     6001 atggctggcc tacggccagg caatctacca gggcgcggac aagccgcgcc gtcgccactc
     6061 gaccgccggc gcccacatca aggcaccctg cctcgcgcgt ttcggtgatg acggtgaaaa
     6121 cctctgacac atgcagctcc cggtgacggt cacagcttgt ctgtaagcgg atgccgggag
     6181 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac
     6241 ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt
     6301 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     6361 cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     6421 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     6481 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     6541 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     6601 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     6661 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     6721 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     6781 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     6841 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     6901 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     6961 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     7021 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     7081 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     7141 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     7201 taagggattt tggtcatgca ttctaggtga ttagaaaaac tcatcgagca tcaaatgaaa
     7261 ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa
     7321 tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc
     7381 gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt
     7441 atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagtttatg
     7501 catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc
     7561 atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agtcgaaata cgcgatcgct
     7621 gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc
     7681 atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg ctgttttccc
     7741 tgggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt
     7801 cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt
     7861 ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa
     7921 tcggtagatt gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa
     7981 atcagcatcc atgttggaat ttaatcgcgg ccttgagcaa gacgtttccc gttgaatatg
     8041 gctcataaca gaacttatta tttccttcct cttttctaca gtatttaaag ataccccaag
     8101 aagctaatta taacaagacg aactccaatt cactgttcct tgcattctaa aaccttaaat
     8161 accagaaaac agctttttca aagttgtttt caaagttggc gtataacata gtatcgacgg
     8221 agccgatttt gaaaccgcgg tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac
     8281 atgctaccct ccgcgagatc atccgtgttt caaacccggc agcttagttg ccgttcttcc
     8341 gaatagcatc ggtaacatga gcaaagtctg ccgccttaca acggctctcc cgctgacgcc
     8401 gtcccggact gatgggctgc ctgtatcgag tggtgatttt gtgccgagct gccggtcggg
     8461 gagctgttgg ctggctggtg gcaggatata ttgtggtgta aacataacga attcgtctca
//
