LOCUS       GB1460                  8526 bp    DNA              UNK 01-JAN-1980
DEFINITION  (GB0030,GB1453,GB1186,GB0037)pDGB3_alpha1
ACCESSION   GB1460
VERSION     GB1460
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        5..1031
                     /vntifkey="30"
                     /label="35S"
     misc_binding    1036..1056
                     /ApEinfo_fwdcolor="#f58a5e"
                     /label="Nuclear Localization Signal (from SV40)"
                     /ApEinfo_revcolor="#f58a5e"
     misc_feature    1063..1427
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /label="PP7delFG"
                     /ApEinfo_revcolor="#ff9ccd"
     CDS             complement(2610..765)
                     /ApEinfo_fwdcolor="#84b0dc"
                     /label="CamR"
                     /ApEinfo_revcolor="#84b0dc"
     misc_feature    1483..1506
                     /ApEinfo_fwdcolor="#b1ff67"
                     /label="NLS"
                     /ApEinfo_revcolor="#b1ff67"
     misc_feature    1459..1482
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /label="NLS"
                     /ApEinfo_revcolor="#ff9ccd"
     misc_feature    1507..1656
                     /ApEinfo_fwdcolor="#85dae9"
                     /label="VP64"
                     /ApEinfo_revcolor="#85dae9"
     misc_feature    1435..1458
                     /ApEinfo_fwdcolor="#85dae9"
                     /label="NLS"
                     /ApEinfo_revcolor="#85dae9"
     mutation        1744
                     /vntifkey="62"
                     /label="T>C"
     terminator      1660..2155
                     /vntifkey="43"
                     /label="Tnos"
     misc_feature    2160..2163
                     /vntifkey="21"
                     /label="GTCA"
     misc_feature    2156..2159
                     /vntifkey="21"
                     /label="CGCT"
     misc_feature    2176..2200
                     /vntifkey="21"
                     /label="RB"
     misc_feature    8485..8510
                     /vntifkey="21"
                     /label="LB"
     CDS             complement(7240..8052)
                     /note="kanamycin resistance gene"
                     /vntifkey="4"
                     /label="KanR"
     rep_origin      complement(6665..6945)
                     /note="pBR322 origin of replication"
                     /vntifkey="33"
                     /label="pBR322\ori"
     misc_feature    complement(6265..6525)
                     /note="bom site from pBR322"
                     /vntifkey="21"
                     /label="pBR322"
     misc_feature    complement(3262..4262)
                     /note="STA region from pVS1 plasmid"
                     /vntifkey="21"
                     /label="STA\region\from\pVS1\plasmid"
     misc_feature    complement(4855..5855)
                     /note="pVS1-REP; replication origin from pVS1"
                     /vntifkey="21"
                     /label="pVS1-Rep\ori"
ORIGIN
        1 ggagactaga gccaagctga tctcctttgc cccggagatc accatggacg actttctcta
       61 tctctacgat ctaggaagaa agttcgacgg agaaggtgac gataccatgt tcaccaccga
      121 taatgagaag attagcctct tcaatttcag aaagaatgct gacccacaga tggttagaga
      181 ggcctacgcg gcaggtctga tcaagacgat ctacccgagt aataatctcc aggagatcaa
      241 ataccttccc aagaaggtta aagatgcagt caaaagattc aggactaact gcatcaagaa
      301 cacagagaaa gatatatttc tcaagatcag aagtactatt ccagtatgga cgattcaagg
      361 cttgcttcat aaaccaaggc aagtaataga gattggagtc tctaagaaag tagttcctac
      421 tgaatcaaag gccatggagt caaaaattca gatcgaggat ctaacagaac tcgccgtgaa
      481 gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga aaatcttcgt
      541 caacatggtg gagcacgaca ctctcgtcta ctccaagaat atcaaagata cagtctcaga
      601 agaccaaagg gctattgaga cttttcaaca aagggtaata tcgggaaacc tcctcggatt
      661 ccattgccca gctatctgtc acttcatcaa aaggacagta gaaaaggaag gtggcaccta
      721 caaatgccat cattgcgata aaggaaaggc tatcgttcaa gatgcctctg ccgacagtgg
      781 tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac
      841 gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg acgcacaatc
      901 ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac
      961 tccggtattt ttacaacaat accacaacaa aacaaacaac aaacaacatt acaatttact
     1021 attctagtcg aaatgcccaa aaagaaaaga aaagtgggta gtatgtccaa aaccatcgtt
     1081 ctttcggtcg gcgaggctac tcgcactctg actgagatcc agtccaccgc agaccgtcag
     1141 atcttcgaag agaaggtcgg gcctctggtg ggtcggctgc gcctcacggc ttcgctccgt
     1201 caaaacggag ccaagaccgc gtatcgcgtc aacctaaaac tggatcaggc ggacgtcgtt
     1261 gattccggac ttccgaaagt gcgctacact caggtatggt cgcacgacgt gacaatcgtt
     1321 gcgaatagca ccgaggcctc gcgcaaatcg ttgtacgatt tgaccaagtc cctcgtcgcg
     1381 acctcgcagg tcgaagatct tgtcgtcaac cttgtgccgc tgggccgtga ttcggaccca
     1441 aaaaagaagc gtaaggtcga tcccaagaag aagagaaagg tagatcctaa gaagaagaga
     1501 aaggtagacg cattggatga cttcgactta gacatgcttg ggtcagatgc tttggacgac
     1561 tttgacctcg acatgttggg ttctgacgcg ctagatgact tcgacttgga catgttgggc
     1621 tccgacgctt tggacgactt tgatctggac atgttatgag cttggaatgg atcttcgatc
     1681 ccgatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg
     1741 cgacgattat catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat
     1801 gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat
     1861 acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc dcggtgtcat
     1921 ctatgttact agatcgggaa ttgccaagct aattcttgaa gacgaaaggg cctcgtgata
     1981 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     2041 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     2101 tatccgctca tgagacaata accctgataa atgcttcaat aatgggaccg actcgcgctg
     2161 tcatgagacg aattctgaca ggatatattg gcgggtaaac ctaagagaaa agagcgttta
     2221 ttagaataat cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg
     2281 tgcatgccaa ccacagggtt cccctcggga tcaaagtact ttgatccaac ccctccgctg
     2341 ctatagtgca gtcggcttct gacgttcagt gcagccgtca tctgaaaacg acatgtcgca
     2401 caagtcctaa gttacgcgac aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc
     2461 gtgttttagt cgcataaagt agaatacttg cgactagaac cggagacatt acgccatgaa
     2521 caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg accaggactt
     2581 gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc aagctgtttt ccgagaagat
     2641 caccggcacc aggcgcgacc gcccggagct ggccaggatg cttgaccacc tacgccctgg
     2701 cgacgttgtg acagtgacca ggctagaccg cctggcccgc agcacccgcg acctactgga
     2761 cattgccgag cgcatccagg aggccggcgc gggcctgcgt agcctggcag agccgtgggc
     2821 cgacaccacc acgccggccg gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt
     2881 cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc gaggccgcca aggcccgagg
     2941 cgtgaagttt ggcccccgcc ctaccctcac cccggcacag atcgcgcacg cccgcgagct
     3001 gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc
     3061 gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg
     3121 cggtgccttc cgtgaggacg cattgaccga ggccgacgcc ctggcggccg ccgagaatga
     3181 acgccaagag gaacaagcat gaaaccgcac caggacggcc aggacgaacc gtttttcatt
     3241 accgaagaga tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac
     3301 ctctcaaccg tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc
     3361 tggccggcca gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta
     3421 tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata tgatccgatg agtaaataaa
     3481 caaatacgca aggggaacgc atgaaggtta tcgctgtact taaccagaaa ggcgggtcag
     3541 gcaagacgac catcggaacc catctagccc gcgccctgca actcgccggg gccgatgttc
     3601 tgttagtcga ttccgatccc cagggcagtg cccgcgattg ggcggccgtg cgggaagatc
     3661 aaccgctaac cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg
     3721 gccggcgcga cttcgtagtg atcgacggag cgccccaggc ggcggacttg gctgtgtccg
     3781 cgatcaaggc agccgacttc gtgctgattc cggtgcagcc aagcccttac gacatatggg
     3841 ccaccgccga cctggtggag ctggttaagc agcgcattga ggtcacggat ggaaggctac
     3901 aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt gaggttgccg
     3961 aggcgctggc cgggtacgag ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct
     4021 acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg
     4081 cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga gttaatgagg
     4141 taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag
     4201 cagcaaggct gcaacgttgg ccagcctggc agacacgcca gccatgaagc gggtcaactt
     4261 tcagttgccg gcggaggatc acaccaagct gaagatgtac gcggtacgcc aaggcaagac
     4321 cattaccgag ctgctatctg aatagatcgc gcagctacca gagtaaatga gcaaatgaat
     4381 aaatgagtag atgaatttta gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag
     4441 gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg
     4501 gctgggttgt ctgccggccc tgcaatggca ctggaacccc caagcccgag gaatcggcgt
     4561 gacggtcgca aaccatccgg cccggtacaa atcggcgcgg cgctgggtga tgacctggtg
     4621 gagaagttga aggccgcgca ggccgcccag cggcaacgca tcgaggcaga agcacgcccc
     4681 ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag aatcccggca accgccggca
     4741 gccggtgcgc cgtcgattag gaagccgccc aagggcgacg agcaaccaga ttttttcgtt
     4801 ccgatgctct atgacgtggg cacccgcgat agtcgcagca tcatggacgt ggccgttttc
     4861 cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc gctacgagct tccagacggg
     4921 cacgtagagg tttccgcagg gccggccggc atggccagtg tgtgggatta cgacctggta
     4981 ctgatggcgg tttcccatct aaccgaatcc atgaaccgat accgggaagg gaagggagac
     5041 aagcccggcc gcgtgttccg tccacacgtt gcggacgtac tcaagttctg ccggcgagcc
     5101 gatggcggaa agcagaaaga cgacctggta gaaacctgca ttcggttaaa caccacgcac
     5161 gttgccatgc agcgtacgaa gaaggccaag aacggccgcc tggtgacggt atccgagggt
     5221 gaagccttga ttagccgcta caagatcgta aagagcgaaa ccgggcggcc ggagtacatc
     5281 gagatcgagc tagctgattg gatgtaccgc gagatcacag aaggcaagaa cccggacgtg
     5341 ctgacggttc accccgatta ctttttgatc gatcccggca tcggccgttt tctctaccgc
     5401 ctggcacgcc gcgccgcagg caaggcagaa gccagatggt tgttcaagac gatctacgaa
     5461 cgcagtggca gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg
     5521 tcaaatgacc tgccggagta cgatttgaag gaggaggcgg ggcaggctgg cccgatccta
     5581 gtcatgcgct accgcaacct gatcgagggc gaagcatccg ccggttccta atgtacggag
     5641 cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggact ctttcctgtg
     5701 gatagcacgt acattgggaa cccaaagccg tacattggga accggaaccc gtacattggg
     5761 aacccaaagc cgtacattgg gaaccggtca cacatgtaag tgactgatat aaaagagaaa
     5821 aaaggcgatt tttccgccta aaactcttta aaacttatta aaactcttaa aacccgcctg
     5881 gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt
     5941 cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc
     6001 tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg
     6061 ccactcgacc gccggcgccc acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg
     6121 tgaaaacctc tgacacatgc agctcccggt gacggtcaca gcttgtctgt aagcggatgc
     6181 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc
     6241 catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag
     6301 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     6361 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     6421 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     6481 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     6541 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     6601 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     6661 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     6721 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     6781 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     6841 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     6901 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     6961 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     7021 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     7081 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     7141 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     7201 tcacgttaag ggattttggt catgcattct aggtgattag aaaaactcat cgagcatcaa
     7261 atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt
     7321 ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat cctggtatcg
     7381 gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat
     7441 aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag
     7501 tttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt catcaaaatc
     7561 actcgcatca accaaaccgt tattcattcg tgattgcgcc tgagcgagtc gaaatacgcg
     7621 atcgctgtta aaaggacaat tacaaacagg aatcgaatgc aaccggcgca ggaacactgc
     7681 cagcgcatca acaatatttt cacctgaatc aggatattct tctaatacct ggaatgctgt
     7741 tttccctggg atcgcagtgg tgagtaacca tgcatcatca ggagtacgga taaaatgctt
     7801 gatggtcgga agaggcataa attccgtcag ccagtttagt ctgaccatct catctgtaac
     7861 atcattggca acgctacctt tgccatgttt cagaaacaac tctggcgcat cgggcttccc
     7921 atacaatcgg tagattgtcg cacctgattg cccgacatta tcgcgagccc atttataccc
     7981 atataaatca gcatccatgt tggaatttaa tcgcggcctt gagcaagacg tttcccgttg
     8041 aatatggctc ataacagaac ttattatttc cttcctcttt tctacagtat ttaaagatac
     8101 cccaagaagc taattataac aagacgaact ccaattcact gttccttgca ttctaaaacc
     8161 ttaaatacca gaaaacagct ttttcaaagt tgttttcaaa gttggcgtat aacatagtat
     8221 cgacggagcc gattttgaaa ccgcggtgat cacaggcagc aacgctctgt catcgttaca
     8281 atcaacatgc taccctccgc gagatcatcc gtgtttcaaa cccggcagct tagttgccgt
     8341 tcttccgaat agcatcggta acatgagcaa agtctgccgc cttacaacgg ctctcccgct
     8401 gacgccgtcc cggactgatg ggctgcctgt atcgagtggt gattttgtgc cgagctgccg
     8461 gtcggggagc tgttggctgg ctggtggcag gatatattgt ggtgtaaaca taacgaattc
     8521 gtctca
//
