LOCUS       GB_UA_1B85              8444 bp    DNA              UNK 01-JAN-1980
DEFINITION  (GB0030,GB1435,GB1187,GB0037)pDGB3_alpha2
ACCESSION   GB_UA_1B85
VERSION     GB_UA_1B85
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        5..1031
                     /vntifkey="30"
                     /label="35S"
     misc_feature    1429..1500
                     /ApEinfo_fwdcolor="#d59687"
                     /label="3nls"
                     /ApEinfo_revcolor="#d59687"
     misc_feature    1453..1476
                     /ApEinfo_fwdcolor="#b1ff67"
                     /label="NLS"
                     /ApEinfo_revcolor="#b1ff67"
     misc_feature    1429..1452
                     /ApEinfo_fwdcolor="#f58a5e"
                     /label="NLS"
                     /ApEinfo_revcolor="#f58a5e"
     misc_feature    1501..1575
                     /ApEinfo_fwdcolor="#d6b295"
                     /label="EDLL"
                     /ApEinfo_revcolor="#d6b295"
     misc_feature    1477..1500
                     /ApEinfo_fwdcolor="#ffef86"
                     /label="NLS"
                     /ApEinfo_revcolor="#ffef86"
     mutation        1660
                     /vntifkey="62"
                     /label="T>C"
     terminator      1576..2071
                     /vntifkey="43"
                     /label="Tnos"
     misc_feature    2089..2113
                     /vntifkey="21"
                     /label="RB"
     misc_feature    8398..8423
                     /vntifkey="21"
                     /label="LB"
     CDS             complement(7153..7965)
                     /note="kanamycin resistance gene"
                     /vntifkey="4"
                     /label="KanR"
     rep_origin      complement(6578..6858)
                     /note="pBR322 origin of replication"
                     /vntifkey="33"
                     /label="pBR322\ori"
     misc_feature    complement(6178..6438)
                     /note="bom site from pBR322"
                     /vntifkey="21"
                     /label="pBR322"
     misc_feature    complement(3175..4175)
                     /note="STA region from pVS1 plasmid"
                     /vntifkey="21"
                     /label="STA\region\from\pVS1\plasmid"
     misc_feature    complement(4768..5768)
                     /note="pVS1-REP; replication origin from pVS1"
                     /vntifkey="21"
                     /label="pVS1-Rep\ori"
ORIGIN
        1 ggagactaga gccaagctga tctcctttgc cccggagatc accatggacg actttctcta
       61 tctctacgat ctaggaagaa agttcgacgg agaaggtgac gataccatgt tcaccaccga
      121 taatgagaag attagcctct tcaatttcag aaagaatgct gacccacaga tggttagaga
      181 ggcctacgcg gcaggtctga tcaagacgat ctacccgagt aataatctcc aggagatcaa
      241 ataccttccc aagaaggtta aagatgcagt caaaagattc aggactaact gcatcaagaa
      301 cacagagaaa gatatatttc tcaagatcag aagtactatt ccagtatgga cgattcaagg
      361 cttgcttcat aaaccaaggc aagtaataga gattggagtc tctaagaaag tagttcctac
      421 tgaatcaaag gccatggagt caaaaattca gatcgaggat ctaacagaac tcgccgtgaa
      481 gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga aaatcttcgt
      541 caacatggtg gagcacgaca ctctcgtcta ctccaagaat atcaaagata cagtctcaga
      601 agaccaaagg gctattgaga cttttcaaca aagggtaata tcgggaaacc tcctcggatt
      661 ccattgccca gctatctgtc acttcatcaa aaggacagta gaaaaggaag gtggcaccta
      721 caaatgccat cattgcgata aaggaaaggc tatcgttcaa gatgcctctg ccgacagtgg
      781 tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac
      841 gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg acgcacaatc
      901 ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac
      961 tccggtattt ttacaacaat accacaacaa aacaaacaac aaacaacatt acaatttact
     1021 attctagtcg aaatgatggc ttctaatttt actcaatttg ttcttgttga taatggtggt
     1081 actggtgatg ttactgttgc tccttctaat tttgctaatg gtgttgctga atggatttct
     1141 tctaattcta gatctcaagc ttataaggtt acttgttctg ttagacaatc ttctgctcaa
     1201 aatagaaagt atactattaa ggttgaagtt cctaaggttg ctactcaaac tgttggtggt
     1261 gttgaacttc ctgttgctgc ttggagatct tatcttaata tggaacttac tattcctatt
     1321 tttgctacta attctgattg tgaacttatt gttaaggcta tgcaaggtct tcttaaggat
     1381 ggtaatccta ttccttctgc tattgctgct aattctggta tttattcgga tcctaaaaag
     1441 aagcgaaagg tagacccgaa gaaaaagagg aaggtagatc caaagaagaa gcgcaaggta
     1501 gaagtgtttg aatttgaata tctggatgat aaagtgctgg aagaactgct ggatagcgaa
     1561 gaacgcaaac gctgagcttg gaatggatct tcgatcccga tcgttcaaac atttggcaat
     1621 aaagtttctt aagattgaat cctgttgccg gtcttgcgac gattatcata taatttctgt
     1681 tgaattacgt taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg
     1741 tttttatgat tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc
     1801 gcgcaaacta ggataaatta tcgcgcdcgg tgtcatctat gttactagat cgggaattgc
     1861 caagctaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     1921 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     1981 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     2041 tgataaatgc ttcaataatg ggaccgactc gcgcttgaga cgaagctttg acaggatata
     2101 ttggcgggta aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc
     2161 gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttcccctcg
     2221 ggatcaaagt actttgatcc aacccctccg ctgctatagt gcagtcggct tctgacgttc
     2281 agtgcagccg tcatctgaaa acgacatgtc gcacaagtcc taagttacgc gacaggctgc
     2341 cgccctgccc ttttcctggc gttttcttgt cgcgtgtttt agtcgcataa agtagaatac
     2401 ttgcgactag aaccggagac attacgccat gaacaagagc gccgccgctg gcctgctggg
     2461 ctatgcccgc gtcagcaccg acgaccagga cttgaccaac caacgggccg aactgcacgc
     2521 ggccggctgc accaagctgt tttccgagaa gatcaccggc accaggcgcg accgcccgga
     2581 gctggccagg atgcttgacc acctacgccc tggcgacgtt gtgacagtga ccaggctaga
     2641 ccgcctggcc cgcagcaccc gcgacctact ggacattgcc gagcgcatcc aggaggccgg
     2701 cgcgggcctg cgtagcctgg cagagccgtg ggccgacacc accacgccgg ccggccgcat
     2761 ggtgttgacc gtgttcgccg gcattgccga gttcgagcgt tccctaatca tcgaccgcac
     2821 ccggagcggg cgcgaggccg ccaaggcccg aggcgtgaag tttggccccc gccctaccct
     2881 caccccggca cagatcgcgc acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa
     2941 agaggcggct gcactgcttg gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag
     3001 cgaggaagtg acgcccaccg aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac
     3061 cgaggccgac gccctggcgg ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg
     3121 caccaggacg gccaggacga accgtttttc attaccgaag agatcgaggc ggagatgatc
     3181 gcggccgggt acgtgttcga gccgcccgcg cacctctcaa ccgtgcggct gcatgaaatc
     3241 ctggccggtt tgtctgatgc caagctggcg gcctggccgg ccagcttggc cgctgaagaa
     3301 accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg
     3361 gtcgctgcgt atatgatccg atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg
     3421 ttatcgctgt acttaaccag aaaggcgggt caggcaagac gaccatcgga acccatctag
     3481 cccgcgccct gcaactcgcc ggggccgatg ttctgttagt cgattccgat ccccagggca
     3541 gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc
     3601 gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg
     3661 gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga
     3721 ttccggtgca gccaagccct tacgacatat gggccaccgc cgacctggtg gagctggtta
     3781 agcagcgcat tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga
     3841 tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac gagctgccca
     3901 ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg cactgccgcc gccggcacaa
     3961 ccgttcttga atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg
     4021 aaattaaatc aaaactcatt tgagttaatg aggtaaagag aaaatgagca aaagcacaaa
     4081 cacgctaagt gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt tggccagcct
     4141 ggcagacacg ccagccatga agcgggtcaa ctttcagttg ccggcggagg atcacaccaa
     4201 gctgaagatg tacgcggtac gccaaggcaa gaccattacc gagctgctat ctgaatagat
     4261 cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag tagatgaatt ttagcggcta
     4321 aaggaggcgg catggaaaat caagaacaac caggcaccga cgccgtggaa tgccccatgt
     4381 gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt tgtctgccgg ccctgcaatg
     4441 gcactggaac ccccaagccc gaggaatcgg cgtgacggtc gcaaaccatc cggcccggta
     4501 caaatcggcg cggcgctggg tgatgacctg gtggagaagt tgaaggccgc gcaggccgcc
     4561 cagcggcaac gcatcgaggc agaagcacgc cccggtgaat cgtggcaagc ggccgctgat
     4621 cgaatccgca aagaatcccg gcaaccgccg gcagccggtg cgccgtcgat taggaagccg
     4681 cccaagggcg acgagcaacc agattttttc gttccgatgc tctatgacgt gggcacccgc
     4741 gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct
     4801 ggcgaggtga tccgctacga gcttccagac gggcacgtag aggtttccgc agggccggcc
     4861 ggcatggcca gtgtgtggga ttacgacctg gtactgatgg cggtttccca tctaaccgaa
     4921 tccatgaacc gataccggga agggaaggga gacaagcccg gccgcgtgtt ccgtccacac
     4981 gttgcggacg tactcaagtt ctgccggcga gccgatggcg gaaagcagaa agacgacctg
     5041 gtagaaacct gcattcggtt aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc
     5101 aagaacggcc gcctggtgac ggtatccgag ggtgaagcct tgattagccg ctacaagatc
     5161 gtaaagagcg aaaccgggcg gccggagtac atcgagatcg agctagctga ttggatgtac
     5221 cgcgagatca cagaaggcaa gaacccggac gtgctgacgg ttcaccccga ttactttttg
     5281 atcgatcccg gcatcggccg ttttctctac cgcctggcac gccgcgccgc aggcaaggca
     5341 gaagccagat ggttgttcaa gacgatctac gaacgcagtg gcagcgccgg agagttcaag
     5401 aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg acctgccgga gtacgatttg
     5461 aaggaggagg cggggcaggc tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag
     5521 ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc tagggcaaat tgccctagca
     5581 ggggaaaaag gtcgaaaagg actctttcct gtggatagca cgtacattgg gaacccaaag
     5641 ccgtacattg ggaaccggaa cccgtacatt gggaacccaa agccgtacat tgggaaccgg
     5701 tcacacatgt aagtgactga tataaaagag aaaaaaggcg atttttccgc ctaaaactct
     5761 ttaaaactta ttaaaactct taaaacccgc ctggcctgtg cataactgtc tggccagcgc
     5821 acagccgaag agctgcaaaa agcgcctacc cttcggtcgc tgcgctccct acgccccgcc
     5881 gcttcgcgtc ggcctatcgc ggccgctggc cgctcaaaaa tggctggcct acggccaggc
     5941 aatctaccag ggcgcggaca agccgcgccg tcgccactcg accgccggcg cccacatcaa
     6001 ggcaccctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
     6061 ggtgacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
     6121 gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg
     6181 agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
     6241 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct
     6301 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     6361 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     6421 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     6481 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     6541 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     6601 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     6661 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     6721 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     6781 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     6841 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     6901 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     6961 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     7021 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     7081 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgcat
     7141 tctaggtgat tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg
     7201 attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag
     7261 gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc
     7321 aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg
     7381 agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc
     7441 aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat
     7501 tcgtgattgc gcctgagcga gtcgaaatac gcgatcgctg ttaaaaggac aattacaaac
     7561 aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga
     7621 atcaggatat tcttctaata cctggaatgc tgttttccct gggatcgcag tggtgagtaa
     7681 ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt
     7741 cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg
     7801 tttcagaaac aactctggcg catcgggctt cccatacaat cggtagattg tcgcacctga
     7861 ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt
     7921 taatcgcggc cttgagcaag acgtttcccg ttgaatatgg ctcataacag aacttattat
     7981 ttccttcctc ttttctacag tatttaaaga taccccaaga agctaattat aacaagacga
     8041 actccaattc actgttcctt gcattctaaa accttaaata ccagaaaaca gctttttcaa
     8101 agttgttttc aaagttggcg tataacatag tatcgacgga gccgattttg aaaccgcggt
     8161 gatcacaggc agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca
     8221 tccgtgtttc aaacccggca gcttagttgc cgttcttccg aatagcatcg gtaacatgag
     8281 caaagtctgc cgccttacaa cggctctccc gctgacgccg tcccggactg atgggctgcc
     8341 tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg agctgttggc tggctggtgg
     8401 caggatatat tgtggtgtaa acataacaag cttcgtctca gtca
//
