LOCUS       GB_UA_1C62              9518 bp    DNA              UNK 01-JAN-1980
DEFINITION  (GB0030,GB1435,GB_UD_4B0C,GB0037)pDGB3_alpha2
ACCESSION   GB_UA_1C62
VERSION     GB_UA_1C62
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        5..1031
                     /vntifkey="30"
                     /label="35S"
     misc_feature    1429..2649
     mutation        2734
                     /vntifkey="62"
                     /label="T>C"
     terminator      2650..3145
                     /vntifkey="43"
                     /label="Tnos"
     misc_feature    3163..3187
                     /vntifkey="21"
                     /label="RB"
     misc_feature    9472..9497
                     /vntifkey="21"
                     /label="LB"
     CDS             complement(8227..9039)
                     /note="kanamycin resistance gene"
                     /vntifkey="4"
                     /label="KanR"
     rep_origin      complement(7652..7932)
                     /note="pBR322 origin of replication"
                     /vntifkey="33"
                     /label="pBR322\ori"
     misc_feature    complement(7252..7512)
                     /note="bom site from pBR322"
                     /vntifkey="21"
                     /label="pBR322"
     misc_feature    complement(4249..5249)
                     /note="STA region from pVS1 plasmid"
                     /vntifkey="21"
                     /label="STA\region\from\pVS1\plasmid"
     misc_feature    complement(5842..6842)
                     /note="pVS1-REP; replication origin from pVS1"
                     /vntifkey="21"
                     /label="pVS1-Rep\ori"
ORIGIN
        1 ggagactaga gccaagctga tctcctttgc cccggagatc accatggacg actttctcta
       61 tctctacgat ctaggaagaa agttcgacgg agaaggtgac gataccatgt tcaccaccga
      121 taatgagaag attagcctct tcaatttcag aaagaatgct gacccacaga tggttagaga
      181 ggcctacgcg gcaggtctga tcaagacgat ctacccgagt aataatctcc aggagatcaa
      241 ataccttccc aagaaggtta aagatgcagt caaaagattc aggactaact gcatcaagaa
      301 cacagagaaa gatatatttc tcaagatcag aagtactatt ccagtatgga cgattcaagg
      361 cttgcttcat aaaccaaggc aagtaataga gattggagtc tctaagaaag tagttcctac
      421 tgaatcaaag gccatggagt caaaaattca gatcgaggat ctaacagaac tcgccgtgaa
      481 gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga aaatcttcgt
      541 caacatggtg gagcacgaca ctctcgtcta ctccaagaat atcaaagata cagtctcaga
      601 agaccaaagg gctattgaga cttttcaaca aagggtaata tcgggaaacc tcctcggatt
      661 ccattgccca gctatctgtc acttcatcaa aaggacagta gaaaaggaag gtggcaccta
      721 caaatgccat cattgcgata aaggaaaggc tatcgttcaa gatgcctctg ccgacagtgg
      781 tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac
      841 gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg acgcacaatc
      901 ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac
      961 tccggtattt ttacaacaat accacaacaa aacaaacaac aaacaacatt acaatttact
     1021 attctagtcg aaatgatggc ttctaatttt actcaatttg ttcttgttga taatggtggt
     1081 actggtgatg ttactgttgc tccttctaat tttgctaatg gtgttgctga atggatttct
     1141 tctaattcta gatctcaagc ttataaggtt acttgttctg ttagacaatc ttctgctcaa
     1201 aatagaaagt atactattaa ggttgaagtt cctaaggttg ctactcaaac tgttggtggt
     1261 gttgaacttc ctgttgctgc ttggagatct tatcttaata tggaacttac tattcctatt
     1321 tttgctacta attctgattg tgaacttatt gttaaggcta tgcaaggtct tcttaaggat
     1381 ggtaatccta ttccttctgc tattgctgct aattctggta tttattcgga cccaaaaaag
     1441 aagcgtaagg tcgatcccaa gaagaagaga aaggtagatc ctaagaagaa gagaaaggta
     1501 gacgcattgg atgacttcga cttagacatg cttgggtcag atgctttgga cgactttgac
     1561 ctcgacatgt tgggttctga cgcgctagat gacttcgact tggacatgtt gggctccgac
     1621 gctttggacg actttgatct ggacatgtta ccgaaaaaga aacgcaaagt tgatcctaag
     1681 aagaagagaa aggtacctac acaggccggc gagggcacac tgtctgaagc tctgctgcag
     1741 ctgcagttcg acgacgagga tctgggagcc ctgctgggaa acagcaccga tcctgccgtg
     1801 ttcaccgacc tggccagcgt ggacaacagc gagttccagc agctgctgaa ccagggcatc
     1861 cctgtggccc ctcacaccac cgagcccatg ctgatggaat accccgaggc catcacccgg
     1921 ctcgtgacag gcgctcagag gcctcctgat ccagctcctg cccctctggg agcaccaggc
     1981 ctgcctaatg gactgctgtc tggcgacgag gacttcagct ctatcgccga tatggatttc
     2041 tcagccttgc tgattaactc tagaagttcc ggatctcggg attccaggga agggatgttt
     2101 ttgccgaagc ctgaggccgg ctccgctatt agtgacgtgt ttgagggccg cgaggtgtgc
     2161 cagccaaaac gaatccggcc atttcatcct ccaggaagtc catgggccaa ccgcccactc
     2221 cccgccagcc tcgcaccaac accaaccggt ccagtacatg agccagtcgg gtcactgacc
     2281 ccggcaccag tccctcagcc actggatcca gcgcccgcag tgactcccga ggccagtcac
     2341 ctgttggagg atcccgatga agaaacgagc caggctgtca aagcccttcg ggagatggcc
     2401 gatactgtga ttccccagaa ggaagaggct gcaatctgtg gccaaatgga cctttcccat
     2461 ccgcccccaa ggggccatct ggatgagctg acaaccacac ttgagtccat gaccgaggat
     2521 ctgaacctgg actcacccct gaccccggaa ttgaacgaga ttctggatac cttcctgaac
     2581 gacgagtgcc tcttgcatgc catgcatatc agcacaggac tgtccatctt cgacacatct
     2641 ctgttttgag cttggaatgg atcttcgatc ccgatcgttc aaacatttgg caataaagtt
     2701 tcttaagatt gaatcctgtt gccggtcttg cgacgattat catataattt ctgttgaatt
     2761 acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta
     2821 tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa
     2881 actaggataa attatcgcgc dcggtgtcat ctatgttact agatcgggaa ttgccaagct
     2941 aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     3001 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     3061 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     3121 atgcttcaat aatgggaccg actcgcgctt gagacgaagc tttgacagga tatattggcg
     3181 ggtaaaccta agagaaaaga gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa
     3241 aggtttatcc gttcgtccat ttgtatgtgc atgccaacca cagggttccc ctcgggatca
     3301 aagtactttg atccaacccc tccgctgcta tagtgcagtc ggcttctgac gttcagtgca
     3361 gccgtcatct gaaaacgaca tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct
     3421 gcccttttcc tggcgttttc ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga
     3481 ctagaaccgg agacattacg ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc
     3541 ccgcgtcagc accgacgacc aggacttgac caaccaacgg gccgaactgc acgcggccgg
     3601 ctgcaccaag ctgttttccg agaagatcac cggcaccagg cgcgaccgcc cggagctggc
     3661 caggatgctt gaccacctac gccctggcga cgttgtgaca gtgaccaggc tagaccgcct
     3721 ggcccgcagc acccgcgacc tactggacat tgccgagcgc atccaggagg ccggcgcggg
     3781 cctgcgtagc ctggcagagc cgtgggccga caccaccacg ccggccggcc gcatggtgtt
     3841 gaccgtgttc gccggcattg ccgagttcga gcgttcccta atcatcgacc gcacccggag
     3901 cgggcgcgag gccgccaagg cccgaggcgt gaagtttggc ccccgcccta ccctcacccc
     3961 ggcacagatc gcgcacgccc gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc
     4021 ggctgcactg cttggcgtgc atcgctcgac cctgtaccgc gcacttgagc gcagcgagga
     4081 agtgacgccc accgaggcca ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc
     4141 cgacgccctg gcggccgccg agaatgaacg ccaagaggaa caagcatgaa accgcaccag
     4201 gacggccagg acgaaccgtt tttcattacc gaagagatcg aggcggagat gatcgcggcc
     4261 gggtacgtgt tcgagccgcc cgcgcacctc tcaaccgtgc ggctgcatga aatcctggcc
     4321 ggtttgtctg atgccaagct ggcggcctgg ccggccagct tggccgctga agaaaccgag
     4381 cgccgccgtc taaaaaggtg atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct
     4441 gcgtatatga tccgatgagt aaataaacaa atacgcaagg ggaacgcatg aaggttatcg
     4501 ctgtacttaa ccagaaaggc gggtcaggca agacgaccat cggaacccat ctagcccgcg
     4561 ccctgcaact cgccggggcc gatgttctgt tagtcgattc cgatccccag ggcagtgccc
     4621 gcgattgggc ggccgtgcgg gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga
     4681 cgattgaccg cgacgtgaag gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc
     4741 cccaggcggc ggacttggct gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg
     4801 tgcagccaag cccttacgac atatgggcca ccgccgacct ggtggagctg gttaagcagc
     4861 gcattgaggt cacggatgga aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag
     4921 gcacgcgcat cggcggtgag gttgccgagg cgctggccgg gtacgagctg cccattcttg
     4981 agtcccgtat cacgcagcgc gtgagctacc caggcactgc cgccgccggc acaaccgttc
     5041 ttgaatcaga acccgagggc gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta
     5101 aatcaaaact catttgagtt aatgaggtaa agagaaaatg agcaaaagca caaacacgct
     5161 aagtgccggc cgtccgagcg cacgcagcag caaggctgca acgttggcca gcctggcaga
     5221 cacgccagcc atgaagcggg tcaactttca gttgccggcg gaggatcaca ccaagctgaa
     5281 gatgtacgcg gtacgccaag gcaagaccat taccgagctg ctatctgaat agatcgcgca
     5341 gctaccagag taaatgagca aatgaataaa tgagtagatg aattttagcg gctaaaggag
     5401 gcggcatgga aaatcaagaa caaccaggca ccgacgccgt ggaatgcccc atgtgtggag
     5461 gaacgggcgg ttggccaggc gtaagcggct gggttgtctg ccggccctgc aatggcactg
     5521 gaacccccaa gcccgaggaa tcggcgtgac ggtcgcaaac catccggccc ggtacaaatc
     5581 ggcgcggcgc tgggtgatga cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg
     5641 caacgcatcg aggcagaagc acgccccggt gaatcgtggc aagcggccgc tgatcgaatc
     5701 cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag
     5761 ggcgacgagc aaccagattt tttcgttccg atgctctatg acgtgggcac ccgcgatagt
     5821 cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag
     5881 gtgatccgct acgagcttcc agacgggcac gtagaggttt ccgcagggcc ggccggcatg
     5941 gccagtgtgt gggattacga cctggtactg atggcggttt cccatctaac cgaatccatg
     6001 aaccgatacc gggaagggaa gggagacaag cccggccgcg tgttccgtcc acacgttgcg
     6061 gacgtactca agttctgccg gcgagccgat ggcggaaagc agaaagacga cctggtagaa
     6121 acctgcattc ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac
     6181 ggccgcctgg tgacggtatc cgagggtgaa gccttgatta gccgctacaa gatcgtaaag
     6241 agcgaaaccg ggcggccgga gtacatcgag atcgagctag ctgattggat gtaccgcgag
     6301 atcacagaag gcaagaaccc ggacgtgctg acggttcacc ccgattactt tttgatcgat
     6361 cccggcatcg gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc
     6421 agatggttgt tcaagacgat ctacgaacgc agtggcagcg ccggagagtt caagaagttc
     6481 tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc cggagtacga tttgaaggag
     6541 gaggcggggc aggctggccc gatcctagtc atgcgctacc gcaacctgat cgagggcgaa
     6601 gcatccgccg gttcctaatg tacggagcag atgctagggc aaattgccct agcaggggaa
     6661 aaaggtcgaa aaggactctt tcctgtggat agcacgtaca ttgggaaccc aaagccgtac
     6721 attgggaacc ggaacccgta cattgggaac ccaaagccgt acattgggaa ccggtcacac
     6781 atgtaagtga ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa
     6841 cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc
     6901 gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg
     6961 cgtcggccta tcgcggccgc tggccgctca aaaatggctg gcctacggcc aggcaatcta
     7021 ccagggcgcg gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc
     7081 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggtgac
     7141 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     7201 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta
     7261 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
     7321 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg
     7381 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     7441 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     7501 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     7561 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     7621 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     7681 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     7741 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     7801 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     7861 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     7921 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     7981 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     8041 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     8101 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     8161 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gcattctagg
     8221 tgattagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc
     8281 aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt
     8341 ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca
     8401 acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac
     8461 gactgaatcc ggtgagaatg gcaaaagttt atgcatttct ttccagactt gttcaacagg
     8521 ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga
     8581 ttgcgcctga gcgagtcgaa atacgcgatc gctgttaaaa ggacaattac aaacaggaat
     8641 cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg
     8701 atattcttct aatacctgga atgctgtttt ccctgggatc gcagtggtga gtaaccatgc
     8761 atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca
     8821 gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag
     8881 aaacaactct ggcgcatcgg gcttcccata caatcggtag attgtcgcac ctgattgccc
     8941 gacattatcg cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg
     9001 cggccttgag caagacgttt cccgttgaat atggctcata acagaactta ttatttcctt
     9061 cctcttttct acagtattta aagatacccc aagaagctaa ttataacaag acgaactcca
     9121 attcactgtt ccttgcattc taaaacctta aataccagaa aacagctttt tcaaagttgt
     9181 tttcaaagtt ggcgtataac atagtatcga cggagccgat tttgaaaccg cggtgatcac
     9241 aggcagcaac gctctgtcat cgttacaatc aacatgctac cctccgcgag atcatccgtg
     9301 tttcaaaccc ggcagcttag ttgccgttct tccgaatagc atcggtaaca tgagcaaagt
     9361 ctgccgcctt acaacggctc tcccgctgac gccgtcccgg actgatgggc tgcctgtatc
     9421 gagtggtgat tttgtgccga gctgccggtc ggggagctgt tggctggctg gtggcaggat
     9481 atattgtggt gtaaacataa caagcttcgt ctcagtca
//
