LOCUS       GB_UA_1C65              8438 bp    DNA              UNK 01-JAN-1980
DEFINITION  (GB0030,GB1435,GB_UD_4B12,GB0037)pDGB3_alpha2
ACCESSION   GB_UA_1C65
VERSION     GB_UA_1C65
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        5..1031
                     /vntifkey="30"
                     /label="35S"
     misc_feature    1429..1569
     mutation        1654
                     /vntifkey="62"
                     /label="T>C"
     terminator      1570..2065
                     /vntifkey="43"
                     /label="Tnos"
     misc_feature    2083..2107
                     /vntifkey="21"
                     /label="RB"
     misc_feature    8392..8417
                     /vntifkey="21"
                     /label="LB"
     CDS             complement(7147..7959)
                     /note="kanamycin resistance gene"
                     /vntifkey="4"
                     /label="KanR"
     rep_origin      complement(6572..6852)
                     /note="pBR322 origin of replication"
                     /vntifkey="33"
                     /label="pBR322\ori"
     misc_feature    complement(6172..6432)
                     /note="bom site from pBR322"
                     /vntifkey="21"
                     /label="pBR322"
     misc_feature    complement(3169..4169)
                     /note="STA region from pVS1 plasmid"
                     /vntifkey="21"
                     /label="STA\region\from\pVS1\plasmid"
     misc_feature    complement(4762..5762)
                     /note="pVS1-REP; replication origin from pVS1"
                     /vntifkey="21"
                     /label="pVS1-Rep\ori"
ORIGIN
        1 ggagactaga gccaagctga tctcctttgc cccggagatc accatggacg actttctcta
       61 tctctacgat ctaggaagaa agttcgacgg agaaggtgac gataccatgt tcaccaccga
      121 taatgagaag attagcctct tcaatttcag aaagaatgct gacccacaga tggttagaga
      181 ggcctacgcg gcaggtctga tcaagacgat ctacccgagt aataatctcc aggagatcaa
      241 ataccttccc aagaaggtta aagatgcagt caaaagattc aggactaact gcatcaagaa
      301 cacagagaaa gatatatttc tcaagatcag aagtactatt ccagtatgga cgattcaagg
      361 cttgcttcat aaaccaaggc aagtaataga gattggagtc tctaagaaag tagttcctac
      421 tgaatcaaag gccatggagt caaaaattca gatcgaggat ctaacagaac tcgccgtgaa
      481 gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga aaatcttcgt
      541 caacatggtg gagcacgaca ctctcgtcta ctccaagaat atcaaagata cagtctcaga
      601 agaccaaagg gctattgaga cttttcaaca aagggtaata tcgggaaacc tcctcggatt
      661 ccattgccca gctatctgtc acttcatcaa aaggacagta gaaaaggaag gtggcaccta
      721 caaatgccat cattgcgata aaggaaaggc tatcgttcaa gatgcctctg ccgacagtgg
      781 tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac
      841 gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg acgcacaatc
      901 ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac
      961 tccggtattt ttacaacaat accacaacaa aacaaacaac aaacaacatt acaatttact
     1021 attctagtcg aaatgatggc ttctaatttt actcaatttg ttcttgttga taatggtggt
     1081 actggtgatg ttactgttgc tccttctaat tttgctaatg gtgttgctga atggatttct
     1141 tctaattcta gatctcaagc ttataaggtt acttgttctg ttagacaatc ttctgctcaa
     1201 aatagaaagt atactattaa ggttgaagtt cctaaggttg ctactcaaac tgttggtggt
     1261 gttgaacttc ctgttgctgc ttggagatct tatcttaata tggaacttac tattcctatt
     1321 tttgctacta attctgattg tgaacttatt gttaaggcta tgcaaggtct tcttaaggat
     1381 ggtaatccta ttccttctgc tattgctgct aattctggta tttattcgcc gaaaaagaaa
     1441 cgcaaagttg atcctaagaa gaagagaaag gtatcagagg acatgttggt gtacggactc
     1501 ctcaaagatg atttccattt tgaaacgtca atgttcgact tgagctgtct ttttgatttt
     1561 ccggcgtgag cttggaatgg atcttcgatc ccgatcgttc aaacatttgg caataaagtt
     1621 tcttaagatt gaatcctgtt gccggtcttg cgacgattat catataattt ctgttgaatt
     1681 acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta
     1741 tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa
     1801 actaggataa attatcgcgc dcggtgtcat ctatgttact agatcgggaa ttgccaagct
     1861 aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     1921 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     1981 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     2041 atgcttcaat aatgggaccg actcgcgctt gagacgaagc tttgacagga tatattggcg
     2101 ggtaaaccta agagaaaaga gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa
     2161 aggtttatcc gttcgtccat ttgtatgtgc atgccaacca cagggttccc ctcgggatca
     2221 aagtactttg atccaacccc tccgctgcta tagtgcagtc ggcttctgac gttcagtgca
     2281 gccgtcatct gaaaacgaca tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct
     2341 gcccttttcc tggcgttttc ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga
     2401 ctagaaccgg agacattacg ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc
     2461 ccgcgtcagc accgacgacc aggacttgac caaccaacgg gccgaactgc acgcggccgg
     2521 ctgcaccaag ctgttttccg agaagatcac cggcaccagg cgcgaccgcc cggagctggc
     2581 caggatgctt gaccacctac gccctggcga cgttgtgaca gtgaccaggc tagaccgcct
     2641 ggcccgcagc acccgcgacc tactggacat tgccgagcgc atccaggagg ccggcgcggg
     2701 cctgcgtagc ctggcagagc cgtgggccga caccaccacg ccggccggcc gcatggtgtt
     2761 gaccgtgttc gccggcattg ccgagttcga gcgttcccta atcatcgacc gcacccggag
     2821 cgggcgcgag gccgccaagg cccgaggcgt gaagtttggc ccccgcccta ccctcacccc
     2881 ggcacagatc gcgcacgccc gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc
     2941 ggctgcactg cttggcgtgc atcgctcgac cctgtaccgc gcacttgagc gcagcgagga
     3001 agtgacgccc accgaggcca ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc
     3061 cgacgccctg gcggccgccg agaatgaacg ccaagaggaa caagcatgaa accgcaccag
     3121 gacggccagg acgaaccgtt tttcattacc gaagagatcg aggcggagat gatcgcggcc
     3181 gggtacgtgt tcgagccgcc cgcgcacctc tcaaccgtgc ggctgcatga aatcctggcc
     3241 ggtttgtctg atgccaagct ggcggcctgg ccggccagct tggccgctga agaaaccgag
     3301 cgccgccgtc taaaaaggtg atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct
     3361 gcgtatatga tccgatgagt aaataaacaa atacgcaagg ggaacgcatg aaggttatcg
     3421 ctgtacttaa ccagaaaggc gggtcaggca agacgaccat cggaacccat ctagcccgcg
     3481 ccctgcaact cgccggggcc gatgttctgt tagtcgattc cgatccccag ggcagtgccc
     3541 gcgattgggc ggccgtgcgg gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga
     3601 cgattgaccg cgacgtgaag gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc
     3661 cccaggcggc ggacttggct gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg
     3721 tgcagccaag cccttacgac atatgggcca ccgccgacct ggtggagctg gttaagcagc
     3781 gcattgaggt cacggatgga aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag
     3841 gcacgcgcat cggcggtgag gttgccgagg cgctggccgg gtacgagctg cccattcttg
     3901 agtcccgtat cacgcagcgc gtgagctacc caggcactgc cgccgccggc acaaccgttc
     3961 ttgaatcaga acccgagggc gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta
     4021 aatcaaaact catttgagtt aatgaggtaa agagaaaatg agcaaaagca caaacacgct
     4081 aagtgccggc cgtccgagcg cacgcagcag caaggctgca acgttggcca gcctggcaga
     4141 cacgccagcc atgaagcggg tcaactttca gttgccggcg gaggatcaca ccaagctgaa
     4201 gatgtacgcg gtacgccaag gcaagaccat taccgagctg ctatctgaat agatcgcgca
     4261 gctaccagag taaatgagca aatgaataaa tgagtagatg aattttagcg gctaaaggag
     4321 gcggcatgga aaatcaagaa caaccaggca ccgacgccgt ggaatgcccc atgtgtggag
     4381 gaacgggcgg ttggccaggc gtaagcggct gggttgtctg ccggccctgc aatggcactg
     4441 gaacccccaa gcccgaggaa tcggcgtgac ggtcgcaaac catccggccc ggtacaaatc
     4501 ggcgcggcgc tgggtgatga cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg
     4561 caacgcatcg aggcagaagc acgccccggt gaatcgtggc aagcggccgc tgatcgaatc
     4621 cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag
     4681 ggcgacgagc aaccagattt tttcgttccg atgctctatg acgtgggcac ccgcgatagt
     4741 cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag
     4801 gtgatccgct acgagcttcc agacgggcac gtagaggttt ccgcagggcc ggccggcatg
     4861 gccagtgtgt gggattacga cctggtactg atggcggttt cccatctaac cgaatccatg
     4921 aaccgatacc gggaagggaa gggagacaag cccggccgcg tgttccgtcc acacgttgcg
     4981 gacgtactca agttctgccg gcgagccgat ggcggaaagc agaaagacga cctggtagaa
     5041 acctgcattc ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac
     5101 ggccgcctgg tgacggtatc cgagggtgaa gccttgatta gccgctacaa gatcgtaaag
     5161 agcgaaaccg ggcggccgga gtacatcgag atcgagctag ctgattggat gtaccgcgag
     5221 atcacagaag gcaagaaccc ggacgtgctg acggttcacc ccgattactt tttgatcgat
     5281 cccggcatcg gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc
     5341 agatggttgt tcaagacgat ctacgaacgc agtggcagcg ccggagagtt caagaagttc
     5401 tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc cggagtacga tttgaaggag
     5461 gaggcggggc aggctggccc gatcctagtc atgcgctacc gcaacctgat cgagggcgaa
     5521 gcatccgccg gttcctaatg tacggagcag atgctagggc aaattgccct agcaggggaa
     5581 aaaggtcgaa aaggactctt tcctgtggat agcacgtaca ttgggaaccc aaagccgtac
     5641 attgggaacc ggaacccgta cattgggaac ccaaagccgt acattgggaa ccggtcacac
     5701 atgtaagtga ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa
     5761 cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc
     5821 gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg
     5881 cgtcggccta tcgcggccgc tggccgctca aaaatggctg gcctacggcc aggcaatcta
     5941 ccagggcgcg gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc
     6001 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggtgac
     6061 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     6121 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta
     6181 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
     6241 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg
     6301 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     6361 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     6421 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     6481 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     6541 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     6601 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     6661 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     6721 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     6781 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     6841 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     6901 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     6961 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     7021 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     7081 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gcattctagg
     7141 tgattagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc
     7201 aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt
     7261 ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca
     7321 acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac
     7381 gactgaatcc ggtgagaatg gcaaaagttt atgcatttct ttccagactt gttcaacagg
     7441 ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga
     7501 ttgcgcctga gcgagtcgaa atacgcgatc gctgttaaaa ggacaattac aaacaggaat
     7561 cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg
     7621 atattcttct aatacctgga atgctgtttt ccctgggatc gcagtggtga gtaaccatgc
     7681 atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca
     7741 gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag
     7801 aaacaactct ggcgcatcgg gcttcccata caatcggtag attgtcgcac ctgattgccc
     7861 gacattatcg cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg
     7921 cggccttgag caagacgttt cccgttgaat atggctcata acagaactta ttatttcctt
     7981 cctcttttct acagtattta aagatacccc aagaagctaa ttataacaag acgaactcca
     8041 attcactgtt ccttgcattc taaaacctta aataccagaa aacagctttt tcaaagttgt
     8101 tttcaaagtt ggcgtataac atagtatcga cggagccgat tttgaaaccg cggtgatcac
     8161 aggcagcaac gctctgtcat cgttacaatc aacatgctac cctccgcgag atcatccgtg
     8221 tttcaaaccc ggcagcttag ttgccgttct tccgaatagc atcggtaaca tgagcaaagt
     8281 ctgccgcctt acaacggctc tcccgctgac gccgtcccgg actgatgggc tgcctgtatc
     8341 gagtggtgat tttgtgccga gctgccggtc ggggagctgt tggctggctg gtggcaggat
     8401 atattgtggt gtaaacataa caagcttcgt ctcagtca
//
