LOCUS       GB_UA_1C68              8885 bp    DNA              UNK 01-JAN-1980
DEFINITION  (GB0030,GB_UD_4B08,GB1187,GB0037)pDGB3_alpha2
ACCESSION   GB_UA_1C68
VERSION     GB_UA_1C68
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        5..1031
                     /vntifkey="30"
                     /label="35S"
     misc_feature    1033..1863
     misc_feature    1870..1941
                     /ApEinfo_fwdcolor="#d59687"
                     /label="3nls"
                     /ApEinfo_revcolor="#d59687"
     misc_feature    1894..1917
                     /ApEinfo_fwdcolor="#b1ff67"
                     /label="NLS"
                     /ApEinfo_revcolor="#b1ff67"
     misc_feature    1870..1893
                     /ApEinfo_fwdcolor="#f58a5e"
                     /label="NLS"
                     /ApEinfo_revcolor="#f58a5e"
     misc_feature    1942..2016
                     /ApEinfo_fwdcolor="#d6b295"
                     /label="EDLL"
                     /ApEinfo_revcolor="#d6b295"
     misc_feature    1918..1941
                     /ApEinfo_fwdcolor="#ffef86"
                     /label="NLS"
                     /ApEinfo_revcolor="#ffef86"
     mutation        2101
                     /vntifkey="62"
                     /label="T>C"
     terminator      2017..2512
                     /vntifkey="43"
                     /label="Tnos"
     misc_feature    2530..2554
                     /vntifkey="21"
                     /label="RB"
     misc_feature    8839..8864
                     /vntifkey="21"
                     /label="LB"
     CDS             complement(7594..8406)
                     /note="kanamycin resistance gene"
                     /vntifkey="4"
                     /label="KanR"
     rep_origin      complement(7019..7299)
                     /note="pBR322 origin of replication"
                     /vntifkey="33"
                     /label="pBR322\ori"
     misc_feature    complement(6619..6879)
                     /note="bom site from pBR322"
                     /vntifkey="21"
                     /label="pBR322"
     misc_feature    complement(3616..4616)
                     /note="STA region from pVS1 plasmid"
                     /vntifkey="21"
                     /label="STA\region\from\pVS1\plasmid"
     misc_feature    complement(5209..6209)
                     /note="pVS1-REP; replication origin from pVS1"
                     /vntifkey="21"
                     /label="pVS1-Rep\ori"
ORIGIN
        1 ggagactaga gccaagctga tctcctttgc cccggagatc accatggacg actttctcta
       61 tctctacgat ctaggaagaa agttcgacgg agaaggtgac gataccatgt tcaccaccga
      121 taatgagaag attagcctct tcaatttcag aaagaatgct gacccacaga tggttagaga
      181 ggcctacgcg gcaggtctga tcaagacgat ctacccgagt aataatctcc aggagatcaa
      241 ataccttccc aagaaggtta aagatgcagt caaaagattc aggactaact gcatcaagaa
      301 cacagagaaa gatatatttc tcaagatcag aagtactatt ccagtatgga cgattcaagg
      361 cttgcttcat aaaccaaggc aagtaataga gattggagtc tctaagaaag tagttcctac
      421 tgaatcaaag gccatggagt caaaaattca gatcgaggat ctaacagaac tcgccgtgaa
      481 gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga aaatcttcgt
      541 caacatggtg gagcacgaca ctctcgtcta ctccaagaat atcaaagata cagtctcaga
      601 agaccaaagg gctattgaga cttttcaaca aagggtaata tcgggaaacc tcctcggatt
      661 ccattgccca gctatctgtc acttcatcaa aaggacagta gaaaaggaag gtggcaccta
      721 caaatgccat cattgcgata aaggaaaggc tatcgttcaa gatgcctctg ccgacagtgg
      781 tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac
      841 gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg acgcacaatc
      901 ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac
      961 tccggtattt ttacaacaat accacaacaa aacaaacaac aaacaacatt acaatttact
     1021 attctagtcg aaatgggccc cgacatcgtg atgacccaga gccccagcag cctgagcgcc
     1081 agcgtgggcg accgcgtgac catcacctgc cgcagcagca ccggcgccgt gaccaccagc
     1141 aactacgcca gctgggtgca ggagaagccc ggcaagctgt tcaagggcct gatcggcggc
     1201 accaacaacc gcgcccccgg cgtgcccagc cgcttcagcg gcagcctgat cggcgacaag
     1261 gccaccctga ccatcagcag cctgcagccc gaggacttcg ccacctactt ctgcgccctg
     1321 tggtacagca accactgggt gttcggccag ggcaccaagg tggagctgaa gcgcggcggc
     1381 ggcggcagcg gcggcggcgg cagcggcggc ggcggcagca gcggcggcgg cagcgaggtg
     1441 aagctgctgg agagcggcgg cggcctggtg cagcccggcg gcagcctgaa gctgagctgc
     1501 gccgtgagcg gcttcagcct gaccgactac ggcgtgaact gggtgcgcca ggcccccggc
     1561 cgcggcctgg agtggatcgg cgtgatctgg ggcgacggca tcaccgacta caacagcgcc
     1621 ctgaaggacc gcttcatcat cagcaaggac aacggcaaga acaccgtgta cctgcagatg
     1681 agcaaggtgc gcagcgacga caccgccctg tactactgcg tgaccggcct gttcgactac
     1741 tggggccagg gcaccctggt gaccgtgagc agctacccat acgatgttcc agattacgct
     1801 ggtggaggcg gaggttctgg gggaggaggt agtggcggtg gtggttcagg aggcggcgga
     1861 agcggttcgg atcctaaaaa gaagcgaaag gtagacccga agaaaaagag gaaggtagat
     1921 ccaaagaaga agcgcaaggt agaagtgttt gaatttgaat atctggatga taaagtgctg
     1981 gaagaactgc tggatagcga agaacgcaaa cgctgagctt ggaatggatc ttcgatcccg
     2041 atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga
     2101 cgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca
     2161 tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg
     2221 cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcdcg gtgtcatcta
     2281 tgttactaga tcgggaattg ccaagctaat tcttgaagac gaaagggcct cgtgatacgc
     2341 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     2401 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     2461 ccgctcatga gacaataacc ctgataaatg cttcaataat gggaccgact cgcgcttgag
     2521 acgaagcttt gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa
     2581 taatcggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg
     2641 ccaaccacag ggttcccctc gggatcaaag tactttgatc caacccctcc gctgctatag
     2701 tgcagtcggc ttctgacgtt cagtgcagcc gtcatctgaa aacgacatgt cgcacaagtc
     2761 ctaagttacg cgacaggctg ccgccctgcc cttttcctgg cgttttcttg tcgcgtgttt
     2821 tagtcgcata aagtagaata cttgcgacta gaaccggaga cattacgcca tgaacaagag
     2881 cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg acttgaccaa
     2941 ccaacgggcc gaactgcacg cggccggctg caccaagctg ttttccgaga agatcaccgg
     3001 caccaggcgc gaccgcccgg agctggccag gatgcttgac cacctacgcc ctggcgacgt
     3061 tgtgacagtg accaggctag accgcctggc ccgcagcacc cgcgacctac tggacattgc
     3121 cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg gcagagccgt gggccgacac
     3181 caccacgccg gccggccgca tggtgttgac cgtgttcgcc ggcattgccg agttcgagcg
     3241 ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc gccaaggccc gaggcgtgaa
     3301 gtttggcccc cgccctaccc tcaccccggc acagatcgcg cacgcccgcg agctgatcga
     3361 ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc gctcgaccct
     3421 gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc gaggccaggc ggcgcggtgc
     3481 cttccgtgag gacgcattga ccgaggccga cgccctggcg gccgccgaga atgaacgcca
     3541 agaggaacaa gcatgaaacc gcaccaggac ggccaggacg aaccgttttt cattaccgaa
     3601 gagatcgagg cggagatgat cgcggccggg tacgtgttcg agccgcccgc gcacctctca
     3661 accgtgcggc tgcatgaaat cctggccggt ttgtctgatg ccaagctggc ggcctggccg
     3721 gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg tgtatttgag
     3781 taaaacagct tgcgtcatgc ggtcgctgcg tatatgatcc gatgagtaaa taaacaaata
     3841 cgcaagggga acgcatgaag gttatcgctg tacttaacca gaaaggcggg tcaggcaaga
     3901 cgaccatcgg aacccatcta gcccgcgccc tgcaactcgc cggggccgat gttctgttag
     3961 tcgattccga tccccagggc agtgcccgcg attgggcggc cgtgcgggaa gatcaaccgc
     4021 taaccgttgt cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc atcggccggc
     4081 gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga cttggctgtg tccgcgatca
     4141 aggcagccga cttcgtgctg attccggtgc agccaagccc ttacgacata tgggccaccg
     4201 ccgacctggt ggagctggtt aagcagcgca ttgaggtcac ggatggaagg ctacaagcgg
     4261 cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt gccgaggcgc
     4321 tggccgggta cgagctgccc attcttgagt cccgtatcac gcagcgcgtg agctacccag
     4381 gcactgccgc cgccggcaca accgttcttg aatcagaacc cgagggcgac gctgcccgcg
     4441 aggtccaggc gctggccgct gaaattaaat caaaactcat ttgagttaat gaggtaaaga
     4501 gaaaatgagc aaaagcacaa acacgctaag tgccggccgt ccgagcgcac gcagcagcaa
     4561 ggctgcaacg ttggccagcc tggcagacac gccagccatg aagcgggtca actttcagtt
     4621 gccggcggag gatcacacca agctgaagat gtacgcggta cgccaaggca agaccattac
     4681 cgagctgcta tctgaataga tcgcgcagct accagagtaa atgagcaaat gaataaatga
     4741 gtagatgaat tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa ccaggcaccg
     4801 acgccgtgga atgccccatg tgtggaggaa cgggcggttg gccaggcgta agcggctggg
     4861 ttgtctgccg gccctgcaat ggcactggaa cccccaagcc cgaggaatcg gcgtgacggt
     4921 cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg gtgatgacct ggtggagaag
     4981 ttgaaggccg cgcaggccgc ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa
     5041 tcgtggcaag cggccgctga tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt
     5101 gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac cagatttttt cgttccgatg
     5161 ctctatgacg tgggcacccg cgatagtcgc agcatcatgg acgtggccgt tttccgtctg
     5221 tcgaagcgtg accgacgagc tggcgaggtg atccgctacg agcttccaga cgggcacgta
     5281 gaggtttccg cagggccggc cggcatggcc agtgtgtggg attacgacct ggtactgatg
     5341 gcggtttccc atctaaccga atccatgaac cgataccggg aagggaaggg agacaagccc
     5401 ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt tctgccggcg agccgatggc
     5461 ggaaagcaga aagacgacct ggtagaaacc tgcattcggt taaacaccac gcacgttgcc
     5521 atgcagcgta cgaagaaggc caagaacggc cgcctggtga cggtatccga gggtgaagcc
     5581 ttgattagcc gctacaagat cgtaaagagc gaaaccgggc ggccggagta catcgagatc
     5641 gagctagctg attggatgta ccgcgagatc acagaaggca agaacccgga cgtgctgacg
     5701 gttcaccccg attacttttt gatcgatccc ggcatcggcc gttttctcta ccgcctggca
     5761 cgccgcgccg caggcaaggc agaagccaga tggttgttca agacgatcta cgaacgcagt
     5821 ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat
     5881 gacctgccgg agtacgattt gaaggaggag gcggggcagg ctggcccgat cctagtcatg
     5941 cgctaccgca acctgatcga gggcgaagca tccgccggtt cctaatgtac ggagcagatg
     6001 ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag gactctttcc tgtggatagc
     6061 acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat tgggaaccca
     6121 aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga gaaaaaaggc
     6181 gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg cctggcctgt
     6241 gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac ccttcggtcg
     6301 ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg ccgctcaaaa
     6361 atggctggcc tacggccagg caatctacca gggcgcggac aagccgcgcc gtcgccactc
     6421 gaccgccggc gcccacatca aggcaccctg cctcgcgcgt ttcggtgatg acggtgaaaa
     6481 cctctgacac atgcagctcc cggtgacggt cacagcttgt ctgtaagcgg atgccgggag
     6541 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac
     6601 ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt
     6661 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     6721 cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     6781 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     6841 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     6901 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     6961 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     7021 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     7081 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     7141 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     7201 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     7261 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     7321 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     7381 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     7441 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     7501 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     7561 taagggattt tggtcatgca ttctaggtga ttagaaaaac tcatcgagca tcaaatgaaa
     7621 ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa
     7681 tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc
     7741 gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt
     7801 atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagtttatg
     7861 catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc
     7921 atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agtcgaaata cgcgatcgct
     7981 gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc
     8041 atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg ctgttttccc
     8101 tgggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt
     8161 cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt
     8221 ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa
     8281 tcggtagatt gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa
     8341 atcagcatcc atgttggaat ttaatcgcgg ccttgagcaa gacgtttccc gttgaatatg
     8401 gctcataaca gaacttatta tttccttcct cttttctaca gtatttaaag ataccccaag
     8461 aagctaatta taacaagacg aactccaatt cactgttcct tgcattctaa aaccttaaat
     8521 accagaaaac agctttttca aagttgtttt caaagttggc gtataacata gtatcgacgg
     8581 agccgatttt gaaaccgcgg tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac
     8641 atgctaccct ccgcgagatc atccgtgttt caaacccggc agcttagttg ccgttcttcc
     8701 gaatagcatc ggtaacatga gcaaagtctg ccgccttaca acggctctcc cgctgacgcc
     8761 gtcccggact gatgggctgc ctgtatcgag tggtgatttt gtgccgagct gccggtcggg
     8821 gagctgttgg ctggctggtg gcaggatata ttgtggtgta aacataacaa gcttcgtctc
     8881 agtca
//
