LOCUS       GB_UA_1C94              8864 bp    DNA              UNK 01-JAN-1980
DEFINITION  (GB0030,GB1435,GB_UD_4C8D,GB0037)pDGB3_alpha2
ACCESSION   GB_UA_1C94
VERSION     GB_UA_1C94
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        5..1031
                     /vntifkey="30"
                     /label="35S"
     misc_feature    1429..1995
     mutation        2080
                     /vntifkey="62"
                     /label="T>C"
     terminator      1996..2491
                     /vntifkey="43"
                     /label="Tnos"
     misc_feature    2509..2533
                     /vntifkey="21"
                     /label="RB"
     misc_feature    8818..8843
                     /vntifkey="21"
                     /label="LB"
     CDS             complement(7573..8385)
                     /note="kanamycin resistance gene"
                     /vntifkey="4"
                     /label="KanR"
     rep_origin      complement(6998..7278)
                     /note="pBR322 origin of replication"
                     /vntifkey="33"
                     /label="pBR322\ori"
     misc_feature    complement(6598..6858)
                     /note="bom site from pBR322"
                     /vntifkey="21"
                     /label="pBR322"
     misc_feature    complement(3595..4595)
                     /note="STA region from pVS1 plasmid"
                     /vntifkey="21"
                     /label="STA\region\from\pVS1\plasmid"
     misc_feature    complement(5188..6188)
                     /note="pVS1-REP; replication origin from pVS1"
                     /vntifkey="21"
                     /label="pVS1-Rep\ori"
ORIGIN
        1 ggagactaga gccaagctga tctcctttgc cccggagatc accatggacg actttctcta
       61 tctctacgat ctaggaagaa agttcgacgg agaaggtgac gataccatgt tcaccaccga
      121 taatgagaag attagcctct tcaatttcag aaagaatgct gacccacaga tggttagaga
      181 ggcctacgcg gcaggtctga tcaagacgat ctacccgagt aataatctcc aggagatcaa
      241 ataccttccc aagaaggtta aagatgcagt caaaagattc aggactaact gcatcaagaa
      301 cacagagaaa gatatatttc tcaagatcag aagtactatt ccagtatgga cgattcaagg
      361 cttgcttcat aaaccaaggc aagtaataga gattggagtc tctaagaaag tagttcctac
      421 tgaatcaaag gccatggagt caaaaattca gatcgaggat ctaacagaac tcgccgtgaa
      481 gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga aaatcttcgt
      541 caacatggtg gagcacgaca ctctcgtcta ctccaagaat atcaaagata cagtctcaga
      601 agaccaaagg gctattgaga cttttcaaca aagggtaata tcgggaaacc tcctcggatt
      661 ccattgccca gctatctgtc acttcatcaa aaggacagta gaaaaggaag gtggcaccta
      721 caaatgccat cattgcgata aaggaaaggc tatcgttcaa gatgcctctg ccgacagtgg
      781 tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac
      841 gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg acgcacaatc
      901 ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac
      961 tccggtattt ttacaacaat accacaacaa aacaaacaac aaacaacatt acaatttact
     1021 attctagtcg aaatgatggc ttctaatttt actcaatttg ttcttgttga taatggtggt
     1081 actggtgatg ttactgttgc tccttctaat tttgctaatg gtgttgctga atggatttct
     1141 tctaattcta gatctcaagc ttataaggtt acttgttctg ttagacaatc ttctgctcaa
     1201 aatagaaagt atactattaa ggttgaagtt cctaaggttg ctactcaaac tgttggtggt
     1261 gttgaacttc ctgttgctgc ttggagatct tatcttaata tggaacttac tattcctatt
     1321 tttgctacta attctgattg tgaacttatt gttaaggcta tgcaaggtct tcttaaggat
     1381 ggtaatccta ttccttctgc tattgctgct aattctggta tttattcgga cccaaaaaag
     1441 aagcgtaagg tcgatcccaa gaagaagaga aaggtagatc ctaagaagaa gagaaaggta
     1501 gacgcattgg atgacttcga cttagacatg cttgggtcag atgctttgga cgactttgac
     1561 ctcgacatgt tgggttctga cgcgctagat gacttcgact tggacatgtt gggctccgac
     1621 gctttggacg actttgatct ggacatgtta tcactaccca gcgctagcga tgacgcattg
     1681 gatgacttcg acttagacat gcttgggtca gatgctttgg acgactttga cctcgacatg
     1741 ttgggttctg acgcgctaga tgacttcgac ttggacatgt tgggctccga cgctttggac
     1801 gactttgatc tggacatgtt atcactaccc agcgctagcg atgacgcatt ggatgacttc
     1861 gacttagaca tgcttgggtc agatgctttg gacgactttg acctcgacat gttgggttct
     1921 gacgcgctag atgacttcga cttggacatg ttgggctccg acgctttgga cgactttgat
     1981 ctggacatgt tatgagcttg gaatggatct tcgatcccga tcgttcaaac atttggcaat
     2041 aaagtttctt aagattgaat cctgttgccg gtcttgcgac gattatcata taatttctgt
     2101 tgaattacgt taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg
     2161 tttttatgat tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc
     2221 gcgcaaacta ggataaatta tcgcgcdcgg tgtcatctat gttactagat cgggaattgc
     2281 caagctaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     2341 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     2401 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     2461 tgataaatgc ttcaataatg ggaccgactc gcgcttgaga cgaagctttg acaggatata
     2521 ttggcgggta aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc
     2581 gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttcccctcg
     2641 ggatcaaagt actttgatcc aacccctccg ctgctatagt gcagtcggct tctgacgttc
     2701 agtgcagccg tcatctgaaa acgacatgtc gcacaagtcc taagttacgc gacaggctgc
     2761 cgccctgccc ttttcctggc gttttcttgt cgcgtgtttt agtcgcataa agtagaatac
     2821 ttgcgactag aaccggagac attacgccat gaacaagagc gccgccgctg gcctgctggg
     2881 ctatgcccgc gtcagcaccg acgaccagga cttgaccaac caacgggccg aactgcacgc
     2941 ggccggctgc accaagctgt tttccgagaa gatcaccggc accaggcgcg accgcccgga
     3001 gctggccagg atgcttgacc acctacgccc tggcgacgtt gtgacagtga ccaggctaga
     3061 ccgcctggcc cgcagcaccc gcgacctact ggacattgcc gagcgcatcc aggaggccgg
     3121 cgcgggcctg cgtagcctgg cagagccgtg ggccgacacc accacgccgg ccggccgcat
     3181 ggtgttgacc gtgttcgccg gcattgccga gttcgagcgt tccctaatca tcgaccgcac
     3241 ccggagcggg cgcgaggccg ccaaggcccg aggcgtgaag tttggccccc gccctaccct
     3301 caccccggca cagatcgcgc acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa
     3361 agaggcggct gcactgcttg gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag
     3421 cgaggaagtg acgcccaccg aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac
     3481 cgaggccgac gccctggcgg ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg
     3541 caccaggacg gccaggacga accgtttttc attaccgaag agatcgaggc ggagatgatc
     3601 gcggccgggt acgtgttcga gccgcccgcg cacctctcaa ccgtgcggct gcatgaaatc
     3661 ctggccggtt tgtctgatgc caagctggcg gcctggccgg ccagcttggc cgctgaagaa
     3721 accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg
     3781 gtcgctgcgt atatgatccg atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg
     3841 ttatcgctgt acttaaccag aaaggcgggt caggcaagac gaccatcgga acccatctag
     3901 cccgcgccct gcaactcgcc ggggccgatg ttctgttagt cgattccgat ccccagggca
     3961 gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc
     4021 gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg
     4081 gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga
     4141 ttccggtgca gccaagccct tacgacatat gggccaccgc cgacctggtg gagctggtta
     4201 agcagcgcat tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga
     4261 tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac gagctgccca
     4321 ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg cactgccgcc gccggcacaa
     4381 ccgttcttga atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg
     4441 aaattaaatc aaaactcatt tgagttaatg aggtaaagag aaaatgagca aaagcacaaa
     4501 cacgctaagt gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt tggccagcct
     4561 ggcagacacg ccagccatga agcgggtcaa ctttcagttg ccggcggagg atcacaccaa
     4621 gctgaagatg tacgcggtac gccaaggcaa gaccattacc gagctgctat ctgaatagat
     4681 cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag tagatgaatt ttagcggcta
     4741 aaggaggcgg catggaaaat caagaacaac caggcaccga cgccgtggaa tgccccatgt
     4801 gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt tgtctgccgg ccctgcaatg
     4861 gcactggaac ccccaagccc gaggaatcgg cgtgacggtc gcaaaccatc cggcccggta
     4921 caaatcggcg cggcgctggg tgatgacctg gtggagaagt tgaaggccgc gcaggccgcc
     4981 cagcggcaac gcatcgaggc agaagcacgc cccggtgaat cgtggcaagc ggccgctgat
     5041 cgaatccgca aagaatcccg gcaaccgccg gcagccggtg cgccgtcgat taggaagccg
     5101 cccaagggcg acgagcaacc agattttttc gttccgatgc tctatgacgt gggcacccgc
     5161 gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct
     5221 ggcgaggtga tccgctacga gcttccagac gggcacgtag aggtttccgc agggccggcc
     5281 ggcatggcca gtgtgtggga ttacgacctg gtactgatgg cggtttccca tctaaccgaa
     5341 tccatgaacc gataccggga agggaaggga gacaagcccg gccgcgtgtt ccgtccacac
     5401 gttgcggacg tactcaagtt ctgccggcga gccgatggcg gaaagcagaa agacgacctg
     5461 gtagaaacct gcattcggtt aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc
     5521 aagaacggcc gcctggtgac ggtatccgag ggtgaagcct tgattagccg ctacaagatc
     5581 gtaaagagcg aaaccgggcg gccggagtac atcgagatcg agctagctga ttggatgtac
     5641 cgcgagatca cagaaggcaa gaacccggac gtgctgacgg ttcaccccga ttactttttg
     5701 atcgatcccg gcatcggccg ttttctctac cgcctggcac gccgcgccgc aggcaaggca
     5761 gaagccagat ggttgttcaa gacgatctac gaacgcagtg gcagcgccgg agagttcaag
     5821 aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg acctgccgga gtacgatttg
     5881 aaggaggagg cggggcaggc tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag
     5941 ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc tagggcaaat tgccctagca
     6001 ggggaaaaag gtcgaaaagg actctttcct gtggatagca cgtacattgg gaacccaaag
     6061 ccgtacattg ggaaccggaa cccgtacatt gggaacccaa agccgtacat tgggaaccgg
     6121 tcacacatgt aagtgactga tataaaagag aaaaaaggcg atttttccgc ctaaaactct
     6181 ttaaaactta ttaaaactct taaaacccgc ctggcctgtg cataactgtc tggccagcgc
     6241 acagccgaag agctgcaaaa agcgcctacc cttcggtcgc tgcgctccct acgccccgcc
     6301 gcttcgcgtc ggcctatcgc ggccgctggc cgctcaaaaa tggctggcct acggccaggc
     6361 aatctaccag ggcgcggaca agccgcgccg tcgccactcg accgccggcg cccacatcaa
     6421 ggcaccctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
     6481 ggtgacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
     6541 gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg
     6601 agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
     6661 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct
     6721 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     6781 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     6841 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     6901 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     6961 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     7021 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     7081 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     7141 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     7201 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     7261 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     7321 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     7381 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     7441 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     7501 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgcat
     7561 tctaggtgat tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg
     7621 attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag
     7681 gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc
     7741 aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg
     7801 agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc
     7861 aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat
     7921 tcgtgattgc gcctgagcga gtcgaaatac gcgatcgctg ttaaaaggac aattacaaac
     7981 aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga
     8041 atcaggatat tcttctaata cctggaatgc tgttttccct gggatcgcag tggtgagtaa
     8101 ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt
     8161 cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg
     8221 tttcagaaac aactctggcg catcgggctt cccatacaat cggtagattg tcgcacctga
     8281 ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt
     8341 taatcgcggc cttgagcaag acgtttcccg ttgaatatgg ctcataacag aacttattat
     8401 ttccttcctc ttttctacag tatttaaaga taccccaaga agctaattat aacaagacga
     8461 actccaattc actgttcctt gcattctaaa accttaaata ccagaaaaca gctttttcaa
     8521 agttgttttc aaagttggcg tataacatag tatcgacgga gccgattttg aaaccgcggt
     8581 gatcacaggc agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca
     8641 tccgtgtttc aaacccggca gcttagttgc cgttcttccg aatagcatcg gtaacatgag
     8701 caaagtctgc cgccttacaa cggctctccc gctgacgccg tcccggactg atgggctgcc
     8761 tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg agctgttggc tggctggtgg
     8821 caggatatat tgtggtgtaa acataacaag cttcgtctca gtca
//
