LOCUS       GB_UA_1FB7              9698 bp    DNA              UNK 01-JAN-1980
DEFINITION  (GB0030,GB1435,GB_UD_553F,GB0037)pDGB3_alpha2
ACCESSION   GB_UA_1FB7
VERSION     GB_UA_1FB7
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        5..1031
                     /vntifkey="30"
                     /label="35S"
     misc_feature    1429..2829
     mutation        2914
                     /vntifkey="62"
                     /label="T>C"
     terminator      2830..3325
                     /vntifkey="43"
                     /label="Tnos"
     misc_feature    3343..3367
                     /vntifkey="21"
                     /label="RB"
     misc_feature    9652..9677
                     /vntifkey="21"
                     /label="LB"
     CDS             complement(8407..9219)
                     /note="kanamycin resistance gene"
                     /vntifkey="4"
                     /label="KanR"
     rep_origin      complement(7832..8112)
                     /note="pBR322 origin of replication"
                     /vntifkey="33"
                     /label="pBR322\ori"
     misc_feature    complement(7432..7692)
                     /note="bom site from pBR322"
                     /vntifkey="21"
                     /label="pBR322"
     misc_feature    complement(4429..5429)
                     /note="STA region from pVS1 plasmid"
                     /vntifkey="21"
                     /label="STA\region\from\pVS1\plasmid"
     misc_feature    complement(6022..7022)
                     /note="pVS1-REP; replication origin from pVS1"
                     /vntifkey="21"
                     /label="pVS1-Rep\ori"
ORIGIN
        1 ggagactaga gccaagctga tctcctttgc cccggagatc accatggacg actttctcta
       61 tctctacgat ctaggaagaa agttcgacgg agaaggtgac gataccatgt tcaccaccga
      121 taatgagaag attagcctct tcaatttcag aaagaatgct gacccacaga tggttagaga
      181 ggcctacgcg gcaggtctga tcaagacgat ctacccgagt aataatctcc aggagatcaa
      241 ataccttccc aagaaggtta aagatgcagt caaaagattc aggactaact gcatcaagaa
      301 cacagagaaa gatatatttc tcaagatcag aagtactatt ccagtatgga cgattcaagg
      361 cttgcttcat aaaccaaggc aagtaataga gattggagtc tctaagaaag tagttcctac
      421 tgaatcaaag gccatggagt caaaaattca gatcgaggat ctaacagaac tcgccgtgaa
      481 gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga aaatcttcgt
      541 caacatggtg gagcacgaca ctctcgtcta ctccaagaat atcaaagata cagtctcaga
      601 agaccaaagg gctattgaga cttttcaaca aagggtaata tcgggaaacc tcctcggatt
      661 ccattgccca gctatctgtc acttcatcaa aaggacagta gaaaaggaag gtggcaccta
      721 caaatgccat cattgcgata aaggaaaggc tatcgttcaa gatgcctctg ccgacagtgg
      781 tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac
      841 gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg acgcacaatc
      901 ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac
      961 tccggtattt ttacaacaat accacaacaa aacaaacaac aaacaacatt acaatttact
     1021 attctagtcg aaatgatggc ttctaatttt actcaatttg ttcttgttga taatggtggt
     1081 actggtgatg ttactgttgc tccttctaat tttgctaatg gtgttgctga atggatttct
     1141 tctaattcta gatctcaagc ttataaggtt acttgttctg ttagacaatc ttctgctcaa
     1201 aatagaaagt atactattaa ggttgaagtt cctaaggttg ctactcaaac tgttggtggt
     1261 gttgaacttc ctgttgctgc ttggagatct tatcttaata tggaacttac tattcctatt
     1321 tttgctacta attctgattg tgaacttatt gttaaggcta tgcaaggtct tcttaaggat
     1381 ggtaatccta ttccttctgc tattgctgct aattctggta tttattcgga cccgaagaaa
     1441 aagaggaagg tagatcctaa gaagaagaga aaggtactgt tggatccggg aacacctatg
     1501 gacgctgatt tggtagcttc ttctacagta gtgtgggaac aagatgcaga tccttttgct
     1561 ggaacagccg acgacttccc cgccttcaat gaagaggagc ttgcatggtt gatggagctg
     1621 ttacctcaag gaggatcagg gggtctttta gatccaggta ctcccatgga tgcagacctg
     1681 gtcgcttcaa gtacagtcgt ttgggagcag gatgctgatc cattcgcagg tacagcagat
     1741 gacttcccgg ctttcaatga ggaggaactt gcttggctca tggaattgct cccgcaggca
     1801 agaggtggat ctggtggtct gctcgaccca ggaacgccga tggacgctga tctggtagca
     1861 tctagtacag tctgggagca ggacgctgac ccattcgctg gtacggctga cgattttcct
     1921 gcctttaatg aggaagagtt agcctggctg atggagttat taccccaggg cggtagtggc
     1981 ggattattgg accctggtac gccgatggat gcagaccttg tggcttcatc aacggtcgta
     2041 tgggagcagg atgccgatcc tttcgcagga accgcagacg acttcccagc ctttaacgaa
     2101 gaagagctcg catggcttat ggaactttta cctcaggcac gtggaggaag tggtgggctc
     2161 ctggatccag gcactccgat ggacgcagac ttagtagcta gttccaccgt tgtctgggag
     2221 caggatgcag atccttttgc tggtaccgca gacgacttcc ctgctttcaa tgaagaagaa
     2281 ttggcctggc ttatggaatt acttccgcag ggtgggtctg gtgggctgct cgatcccggt
     2341 acgccgatgg atgctgactt ggttgctagc agtactgtgg tctgggaaca ggatgctgac
     2401 cccttcgctg ggactgccga cgacttccca gccttcaacg aagaggaact cgcatggttg
     2461 atggaactgc ttcctcaagc acgtgggggg tcaggcggag gcggatctgg cggtgacgca
     2521 ctggacgact ttgatctcga catgcttggg tccgatgctc tcgacgattt tgacctcgat
     2581 atgcttgggt ctgacgcatt agatgacttt gatttagaca tgttggggtc agacgctttg
     2641 gacgatttcg acttagatat gttggccaga ggtagtgatg cccttgacga cttcgattta
     2701 gatatgttgg gctctgacgc cctcgatgac ttcgatctgg atatgctcgg aagtgacgcc
     2761 ttggacgatt tcgacttgga catgcttggg tcggacgccc tcgacgactt tgatctggat
     2821 atgttatgag cttggaatgg atcttcgatc ccgatcgttc aaacatttgg caataaagtt
     2881 tcttaagatt gaatcctgtt gccggtcttg cgacgattat catataattt ctgttgaatt
     2941 acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta
     3001 tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa
     3061 actaggataa attatcgcgc dcggtgtcat ctatgttact agatcgggaa ttgccaagct
     3121 aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     3181 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     3241 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     3301 atgcttcaat aatgggaccg actcgcgctt gagacgaagc tttgacagga tatattggcg
     3361 ggtaaaccta agagaaaaga gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa
     3421 aggtttatcc gttcgtccat ttgtatgtgc atgccaacca cagggttccc ctcgggatca
     3481 aagtactttg atccaacccc tccgctgcta tagtgcagtc ggcttctgac gttcagtgca
     3541 gccgtcatct gaaaacgaca tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct
     3601 gcccttttcc tggcgttttc ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga
     3661 ctagaaccgg agacattacg ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc
     3721 ccgcgtcagc accgacgacc aggacttgac caaccaacgg gccgaactgc acgcggccgg
     3781 ctgcaccaag ctgttttccg agaagatcac cggcaccagg cgcgaccgcc cggagctggc
     3841 caggatgctt gaccacctac gccctggcga cgttgtgaca gtgaccaggc tagaccgcct
     3901 ggcccgcagc acccgcgacc tactggacat tgccgagcgc atccaggagg ccggcgcggg
     3961 cctgcgtagc ctggcagagc cgtgggccga caccaccacg ccggccggcc gcatggtgtt
     4021 gaccgtgttc gccggcattg ccgagttcga gcgttcccta atcatcgacc gcacccggag
     4081 cgggcgcgag gccgccaagg cccgaggcgt gaagtttggc ccccgcccta ccctcacccc
     4141 ggcacagatc gcgcacgccc gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc
     4201 ggctgcactg cttggcgtgc atcgctcgac cctgtaccgc gcacttgagc gcagcgagga
     4261 agtgacgccc accgaggcca ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc
     4321 cgacgccctg gcggccgccg agaatgaacg ccaagaggaa caagcatgaa accgcaccag
     4381 gacggccagg acgaaccgtt tttcattacc gaagagatcg aggcggagat gatcgcggcc
     4441 gggtacgtgt tcgagccgcc cgcgcacctc tcaaccgtgc ggctgcatga aatcctggcc
     4501 ggtttgtctg atgccaagct ggcggcctgg ccggccagct tggccgctga agaaaccgag
     4561 cgccgccgtc taaaaaggtg atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct
     4621 gcgtatatga tccgatgagt aaataaacaa atacgcaagg ggaacgcatg aaggttatcg
     4681 ctgtacttaa ccagaaaggc gggtcaggca agacgaccat cggaacccat ctagcccgcg
     4741 ccctgcaact cgccggggcc gatgttctgt tagtcgattc cgatccccag ggcagtgccc
     4801 gcgattgggc ggccgtgcgg gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga
     4861 cgattgaccg cgacgtgaag gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc
     4921 cccaggcggc ggacttggct gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg
     4981 tgcagccaag cccttacgac atatgggcca ccgccgacct ggtggagctg gttaagcagc
     5041 gcattgaggt cacggatgga aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag
     5101 gcacgcgcat cggcggtgag gttgccgagg cgctggccgg gtacgagctg cccattcttg
     5161 agtcccgtat cacgcagcgc gtgagctacc caggcactgc cgccgccggc acaaccgttc
     5221 ttgaatcaga acccgagggc gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta
     5281 aatcaaaact catttgagtt aatgaggtaa agagaaaatg agcaaaagca caaacacgct
     5341 aagtgccggc cgtccgagcg cacgcagcag caaggctgca acgttggcca gcctggcaga
     5401 cacgccagcc atgaagcggg tcaactttca gttgccggcg gaggatcaca ccaagctgaa
     5461 gatgtacgcg gtacgccaag gcaagaccat taccgagctg ctatctgaat agatcgcgca
     5521 gctaccagag taaatgagca aatgaataaa tgagtagatg aattttagcg gctaaaggag
     5581 gcggcatgga aaatcaagaa caaccaggca ccgacgccgt ggaatgcccc atgtgtggag
     5641 gaacgggcgg ttggccaggc gtaagcggct gggttgtctg ccggccctgc aatggcactg
     5701 gaacccccaa gcccgaggaa tcggcgtgac ggtcgcaaac catccggccc ggtacaaatc
     5761 ggcgcggcgc tgggtgatga cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg
     5821 caacgcatcg aggcagaagc acgccccggt gaatcgtggc aagcggccgc tgatcgaatc
     5881 cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag
     5941 ggcgacgagc aaccagattt tttcgttccg atgctctatg acgtgggcac ccgcgatagt
     6001 cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag
     6061 gtgatccgct acgagcttcc agacgggcac gtagaggttt ccgcagggcc ggccggcatg
     6121 gccagtgtgt gggattacga cctggtactg atggcggttt cccatctaac cgaatccatg
     6181 aaccgatacc gggaagggaa gggagacaag cccggccgcg tgttccgtcc acacgttgcg
     6241 gacgtactca agttctgccg gcgagccgat ggcggaaagc agaaagacga cctggtagaa
     6301 acctgcattc ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac
     6361 ggccgcctgg tgacggtatc cgagggtgaa gccttgatta gccgctacaa gatcgtaaag
     6421 agcgaaaccg ggcggccgga gtacatcgag atcgagctag ctgattggat gtaccgcgag
     6481 atcacagaag gcaagaaccc ggacgtgctg acggttcacc ccgattactt tttgatcgat
     6541 cccggcatcg gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc
     6601 agatggttgt tcaagacgat ctacgaacgc agtggcagcg ccggagagtt caagaagttc
     6661 tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc cggagtacga tttgaaggag
     6721 gaggcggggc aggctggccc gatcctagtc atgcgctacc gcaacctgat cgagggcgaa
     6781 gcatccgccg gttcctaatg tacggagcag atgctagggc aaattgccct agcaggggaa
     6841 aaaggtcgaa aaggactctt tcctgtggat agcacgtaca ttgggaaccc aaagccgtac
     6901 attgggaacc ggaacccgta cattgggaac ccaaagccgt acattgggaa ccggtcacac
     6961 atgtaagtga ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa
     7021 cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc
     7081 gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg
     7141 cgtcggccta tcgcggccgc tggccgctca aaaatggctg gcctacggcc aggcaatcta
     7201 ccagggcgcg gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc
     7261 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggtgac
     7321 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     7381 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta
     7441 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
     7501 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg
     7561 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     7621 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     7681 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     7741 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     7801 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     7861 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     7921 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     7981 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     8041 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     8101 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     8161 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     8221 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     8281 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     8341 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gcattctagg
     8401 tgattagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc
     8461 aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt
     8521 ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca
     8581 acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac
     8641 gactgaatcc ggtgagaatg gcaaaagttt atgcatttct ttccagactt gttcaacagg
     8701 ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga
     8761 ttgcgcctga gcgagtcgaa atacgcgatc gctgttaaaa ggacaattac aaacaggaat
     8821 cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg
     8881 atattcttct aatacctgga atgctgtttt ccctgggatc gcagtggtga gtaaccatgc
     8941 atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca
     9001 gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag
     9061 aaacaactct ggcgcatcgg gcttcccata caatcggtag attgtcgcac ctgattgccc
     9121 gacattatcg cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg
     9181 cggccttgag caagacgttt cccgttgaat atggctcata acagaactta ttatttcctt
     9241 cctcttttct acagtattta aagatacccc aagaagctaa ttataacaag acgaactcca
     9301 attcactgtt ccttgcattc taaaacctta aataccagaa aacagctttt tcaaagttgt
     9361 tttcaaagtt ggcgtataac atagtatcga cggagccgat tttgaaaccg cggtgatcac
     9421 aggcagcaac gctctgtcat cgttacaatc aacatgctac cctccgcgag atcatccgtg
     9481 tttcaaaccc ggcagcttag ttgccgttct tccgaatagc atcggtaaca tgagcaaagt
     9541 ctgccgcctt acaacggctc tcccgctgac gccgtcccgg actgatgggc tgcctgtatc
     9601 gagtggtgat tttgtgccga gctgccggtc ggggagctgt tggctggctg gtggcaggat
     9661 atattgtggt gtaaacataa caagcttcgt ctcagtca
//
