LOCUS       GB_UA_257A              8385 bp    DNA              UNK 01-JAN-1980
DEFINITION  (GB0030,GB_UD_4952,GB01186,GB0037)pDGB3_alpha1
ACCESSION   GB_UA_257A
VERSION     GB_UA_257A
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        5..1031
                     /vntifkey="30"
                     /label="35S"
     misc_feature    1033..1287
     misc_feature    1342..1365
                     /ApEinfo_fwdcolor="#b1ff67"
                     /label="NLS"
                     /ApEinfo_revcolor="#b1ff67"
     misc_feature    1318..1341
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /label="NLS"
                     /ApEinfo_revcolor="#ff9ccd"
     misc_feature    1366..1515
                     /ApEinfo_fwdcolor="#85dae9"
                     /label="VP64"
                     /ApEinfo_revcolor="#85dae9"
     misc_feature    1294..1317
                     /ApEinfo_fwdcolor="#85dae9"
                     /label="NLS"
                     /ApEinfo_revcolor="#85dae9"
     mutation        1603
                     /vntifkey="62"
                     /label="T>C"
     terminator      1519..2014
                     /vntifkey="43"
                     /label="Tnos"
     misc_feature    2019..2022
                     /vntifkey="21"
                     /label="GTCA"
     misc_feature    2015..2018
                     /vntifkey="21"
                     /label="CGCT"
     misc_feature    2035..2059
                     /vntifkey="21"
                     /label="RB"
     misc_feature    8344..8369
                     /vntifkey="21"
                     /label="LB"
     CDS             complement(7099..7911)
                     /note="kanamycin resistance gene"
                     /vntifkey="4"
                     /label="KanR"
     rep_origin      complement(6524..6804)
                     /note="pBR322 origin of replication"
                     /vntifkey="33"
                     /label="pBR322\ori"
     misc_feature    complement(6124..6384)
                     /note="bom site from pBR322"
                     /vntifkey="21"
                     /label="pBR322"
     misc_feature    complement(3121..4121)
                     /note="STA region from pVS1 plasmid"
                     /vntifkey="21"
                     /label="STA\region\from\pVS1\plasmid"
     misc_feature    complement(4714..5714)
                     /note="pVS1-REP; replication origin from pVS1"
                     /vntifkey="21"
                     /label="pVS1-Rep\ori"
ORIGIN
        1 ggagactaga gccaagctga tctcctttgc cccggagatc accatggacg actttctcta
       61 tctctacgat ctaggaagaa agttcgacgg agaaggtgac gataccatgt tcaccaccga
      121 taatgagaag attagcctct tcaatttcag aaagaatgct gacccacaga tggttagaga
      181 ggcctacgcg gcaggtctga tcaagacgat ctacccgagt aataatctcc aggagatcaa
      241 ataccttccc aagaaggtta aagatgcagt caaaagattc aggactaact gcatcaagaa
      301 cacagagaaa gatatatttc tcaagatcag aagtactatt ccagtatgga cgattcaagg
      361 cttgcttcat aaaccaaggc aagtaataga gattggagtc tctaagaaag tagttcctac
      421 tgaatcaaag gccatggagt caaaaattca gatcgaggat ctaacagaac tcgccgtgaa
      481 gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga aaatcttcgt
      541 caacatggtg gagcacgaca ctctcgtcta ctccaagaat atcaaagata cagtctcaga
      601 agaccaaagg gctattgaga cttttcaaca aagggtaata tcgggaaacc tcctcggatt
      661 ccattgccca gctatctgtc acttcatcaa aaggacagta gaaaaggaag gtggcaccta
      721 caaatgccat cattgcgata aaggaaaggc tatcgttcaa gatgcctctg ccgacagtgg
      781 tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac
      841 gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg acgcacaatc
      901 ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac
      961 tccggtattt ttacaacaat accacaacaa aacaaacaac aaacaacatt acaatttact
     1021 attctagtcg aaatgcccaa aaagaaaaga aaagtgggta gtatgaaatc aattcgctgt
     1081 aaaaactgca acaaactgtt atttaaggcg gatagttttg atcacattga aatcaggtgt
     1141 ccgcgttgca aacgtcacat cataatgctg aatgcctgcg agcatcccac ggagaaacat
     1201 tgtgggaaaa gagaaaaaat cacgcattct gacgaaaccg tgcgttatgg ctccactagt
     1261 gggcacaagc ttaatggcgg tggcggaggt tcggacccaa aaaagaagcg taaggtcgat
     1321 cccaagaaga agagaaaggt agatcctaag aagaagagaa aggtagacgc attggatgac
     1381 ttcgacttag acatgcttgg gtcagatgct ttggacgact ttgacctcga catgttgggt
     1441 tctgacgcgc tagatgactt cgacttggac atgttgggct ccgacgcttt ggacgacttt
     1501 gatctggaca tgttatgagc ttggaatgga tcttcgatcc cgatcgttca aacatttggc
     1561 aataaagttt cttaagattg aatcctgttg ccggtcttgc gacgattatc atataatttc
     1621 tgttgaatta cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat
     1681 gggtttttat gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat
     1741 agcgcgcaaa ctaggataaa ttatcgcgcd cggtgtcatc tatgttacta gatcgggaat
     1801 tgccaagcta attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     1861 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     1921 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     1981 ccctgataaa tgcttcaata atgggaccga ctcgcgctgt catgagacga attctgacag
     2041 gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataatc ggatatttaa
     2101 aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac cacagggttc
     2161 ccctcgggat caaagtactt tgatccaacc cctccgctgc tatagtgcag tcggcttctg
     2221 acgttcagtg cagccgtcat ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca
     2281 ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc gcataaagta
     2341 gaatacttgc gactagaacc ggagacatta cgccatgaac aagagcgccg ccgctggcct
     2401 gctgggctat gcccgcgtca gcaccgacga ccaggacttg accaaccaac gggccgaact
     2461 gcacgcggcc ggctgcacca agctgttttc cgagaagatc accggcacca ggcgcgaccg
     2521 cccggagctg gccaggatgc ttgaccacct acgccctggc gacgttgtga cagtgaccag
     2581 gctagaccgc ctggcccgca gcacccgcga cctactggac attgccgagc gcatccagga
     2641 ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc gacaccacca cgccggccgg
     2701 ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc gagcgttccc taatcatcga
     2761 ccgcacccgg agcgggcgcg aggccgccaa ggcccgaggc gtgaagtttg gcccccgccc
     2821 taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac
     2881 cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg accctgtacc gcgcacttga
     2941 gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc
     3001 attgaccgag gccgacgccc tggcggccgc cgagaatgaa cgccaagagg aacaagcatg
     3061 aaaccgcacc aggacggcca ggacgaaccg tttttcatta ccgaagagat cgaggcggag
     3121 atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacc tctcaaccgt gcggctgcat
     3181 gaaatcctgg ccggtttgtc tgatgccaag ctggcggcct ggccggccag cttggccgct
     3241 gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt
     3301 catgcggtcg ctgcgtatat gatccgatga gtaaataaac aaatacgcaa ggggaacgca
     3361 tgaaggttat cgctgtactt aaccagaaag gcgggtcagg caagacgacc atcggaaccc
     3421 atctagcccg cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat tccgatcccc
     3481 agggcagtgc ccgcgattgg gcggccgtgc gggaagatca accgctaacc gttgtcggca
     3541 tcgaccgccc gacgattgac cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga
     3601 tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg
     3661 tgctgattcc ggtgcagcca agcccttacg acatatgggc caccgccgac ctggtggagc
     3721 tggttaagca gcgcattgag gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc
     3781 gggcgatcaa aggcacgcgc atcggcggtg aggttgccga ggcgctggcc gggtacgagc
     3841 tgcccattct tgagtcccgt atcacgcagc gcgtgagcta cccaggcact gccgccgccg
     3901 gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg
     3961 ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag
     4021 cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg caacgttggc
     4081 cagcctggca gacacgccag ccatgaagcg ggtcaacttt cagttgccgg cggaggatca
     4141 caccaagctg aagatgtacg cggtacgcca aggcaagacc attaccgagc tgctatctga
     4201 atagatcgcg cagctaccag agtaaatgag caaatgaata aatgagtaga tgaattttag
     4261 cggctaaagg aggcggcatg gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc
     4321 ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg ctgggttgtc tgccggccct
     4381 gcaatggcac tggaaccccc aagcccgagg aatcggcgtg acggtcgcaa accatccggc
     4441 ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg agaagttgaa ggccgcgcag
     4501 gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc
     4561 gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg
     4621 aagccgccca agggcgacga gcaaccagat tttttcgttc cgatgctcta tgacgtgggc
     4681 acccgcgata gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga
     4741 cgagctggcg aggtgatccg ctacgagctt ccagacgggc acgtagaggt ttccgcaggg
     4801 ccggccggca tggccagtgt gtgggattac gacctggtac tgatggcggt ttcccatcta
     4861 accgaatcca tgaaccgata ccgggaaggg aagggagaca agcccggccg cgtgttccgt
     4921 ccacacgttg cggacgtact caagttctgc cggcgagccg atggcggaaa gcagaaagac
     4981 gacctggtag aaacctgcat tcggttaaac accacgcacg ttgccatgca gcgtacgaag
     5041 aaggccaaga acggccgcct ggtgacggta tccgagggtg aagccttgat tagccgctac
     5101 aagatcgtaa agagcgaaac cgggcggccg gagtacatcg agatcgagct agctgattgg
     5161 atgtaccgcg agatcacaga aggcaagaac ccggacgtgc tgacggttca ccccgattac
     5221 tttttgatcg atcccggcat cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc
     5281 aaggcagaag ccagatggtt gttcaagacg atctacgaac gcagtggcag cgccggagag
     5341 ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct gccggagtac
     5401 gatttgaagg aggaggcggg gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg
     5461 atcgagggcg aagcatccgc cggttcctaa tgtacggagc agatgctagg gcaaattgcc
     5521 ctagcagggg aaaaaggtcg aaaaggactc tttcctgtgg atagcacgta cattgggaac
     5581 ccaaagccgt acattgggaa ccggaacccg tacattggga acccaaagcc gtacattggg
     5641 aaccggtcac acatgtaagt gactgatata aaagagaaaa aaggcgattt ttccgcctaa
     5701 aactctttaa aacttattaa aactcttaaa acccgcctgg cctgtgcata actgtctggc
     5761 cagcgcacag ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc
     5821 cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc tggcctacgg
     5881 ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca
     5941 catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
     6001 gctcccggtg acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
     6061 gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga
     6121 tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac
     6181 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct
     6241 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     6301 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     6361 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     6421 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     6481 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     6541 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     6601 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     6661 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     6721 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     6781 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     6841 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     6901 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     6961 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     7021 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     7081 atgcattcta ggtgattaga aaaactcatc gagcatcaaa tgaaactgca atttattcat
     7141 atcaggatta tcaataccat atttttgaaa aagccgtttc tgtaatgaag gagaaaactc
     7201 accgaggcag ttccatagga tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc
     7261 aacatcaata caacctatta atttcccctc gtcaaaaata aggttatcaa gtgagaaatc
     7321 accatgagtg acgactgaat ccggtgagaa tggcaaaagt ttatgcattt ctttccagac
     7381 ttgttcaaca ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt
     7441 attcattcgt gattgcgcct gagcgagtcg aaatacgcga tcgctgttaa aaggacaatt
     7501 acaaacagga atcgaatgca accggcgcag gaacactgcc agcgcatcaa caatattttc
     7561 acctgaatca ggatattctt ctaatacctg gaatgctgtt ttccctggga tcgcagtggt
     7621 gagtaaccat gcatcatcag gagtacggat aaaatgcttg atggtcggaa gaggcataaa
     7681 ttccgtcagc cagtttagtc tgaccatctc atctgtaaca tcattggcaa cgctaccttt
     7741 gccatgtttc agaaacaact ctggcgcatc gggcttccca tacaatcggt agattgtcgc
     7801 acctgattgc ccgacattat cgcgagccca tttataccca tataaatcag catccatgtt
     7861 ggaatttaat cgcggccttg agcaagacgt ttcccgttga atatggctca taacagaact
     7921 tattatttcc ttcctctttt ctacagtatt taaagatacc ccaagaagct aattataaca
     7981 agacgaactc caattcactg ttccttgcat tctaaaacct taaataccag aaaacagctt
     8041 tttcaaagtt gttttcaaag ttggcgtata acatagtatc gacggagccg attttgaaac
     8101 cgcggtgatc acaggcagca acgctctgtc atcgttacaa tcaacatgct accctccgcg
     8161 agatcatccg tgtttcaaac ccggcagctt agttgccgtt cttccgaata gcatcggtaa
     8221 catgagcaaa gtctgccgcc ttacaacggc tctcccgctg acgccgtccc ggactgatgg
     8281 gctgcctgta tcgagtggtg attttgtgcc gagctgccgg tcggggagct gttggctggc
     8341 tggtggcagg atatattgtg gtgtaaacat aacgaattcg tctca
//
