LOCUS       GB_UA_258C              8961 bp    DNA              UNK 01-JAN-1980
DEFINITION  (GB0030,GB_UD_4B08,GB01186,GB0037)pDGB3_alpha1
ACCESSION   GB_UA_258C
VERSION     GB_UA_258C
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        5..1031
                     /vntifkey="30"
                     /label="35S"
     misc_feature    1033..1863
     misc_feature    1918..1941
                     /ApEinfo_fwdcolor="#b1ff67"
                     /label="NLS"
                     /ApEinfo_revcolor="#b1ff67"
     misc_feature    1894..1917
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /label="NLS"
                     /ApEinfo_revcolor="#ff9ccd"
     misc_feature    1942..2091
                     /ApEinfo_fwdcolor="#85dae9"
                     /label="VP64"
                     /ApEinfo_revcolor="#85dae9"
     misc_feature    1870..1893
                     /ApEinfo_fwdcolor="#85dae9"
                     /label="NLS"
                     /ApEinfo_revcolor="#85dae9"
     mutation        2179
                     /vntifkey="62"
                     /label="T>C"
     terminator      2095..2590
                     /vntifkey="43"
                     /label="Tnos"
     misc_feature    2595..2598
                     /vntifkey="21"
                     /label="GTCA"
     misc_feature    2591..2594
                     /vntifkey="21"
                     /label="CGCT"
     misc_feature    2611..2635
                     /vntifkey="21"
                     /label="RB"
     misc_feature    8920..8945
                     /vntifkey="21"
                     /label="LB"
     CDS             complement(7675..8487)
                     /note="kanamycin resistance gene"
                     /vntifkey="4"
                     /label="KanR"
     rep_origin      complement(7100..7380)
                     /note="pBR322 origin of replication"
                     /vntifkey="33"
                     /label="pBR322\ori"
     misc_feature    complement(6700..6960)
                     /note="bom site from pBR322"
                     /vntifkey="21"
                     /label="pBR322"
     misc_feature    complement(3697..4697)
                     /note="STA region from pVS1 plasmid"
                     /vntifkey="21"
                     /label="STA\region\from\pVS1\plasmid"
     misc_feature    complement(5290..6290)
                     /note="pVS1-REP; replication origin from pVS1"
                     /vntifkey="21"
                     /label="pVS1-Rep\ori"
ORIGIN
        1 ggagactaga gccaagctga tctcctttgc cccggagatc accatggacg actttctcta
       61 tctctacgat ctaggaagaa agttcgacgg agaaggtgac gataccatgt tcaccaccga
      121 taatgagaag attagcctct tcaatttcag aaagaatgct gacccacaga tggttagaga
      181 ggcctacgcg gcaggtctga tcaagacgat ctacccgagt aataatctcc aggagatcaa
      241 ataccttccc aagaaggtta aagatgcagt caaaagattc aggactaact gcatcaagaa
      301 cacagagaaa gatatatttc tcaagatcag aagtactatt ccagtatgga cgattcaagg
      361 cttgcttcat aaaccaaggc aagtaataga gattggagtc tctaagaaag tagttcctac
      421 tgaatcaaag gccatggagt caaaaattca gatcgaggat ctaacagaac tcgccgtgaa
      481 gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga aaatcttcgt
      541 caacatggtg gagcacgaca ctctcgtcta ctccaagaat atcaaagata cagtctcaga
      601 agaccaaagg gctattgaga cttttcaaca aagggtaata tcgggaaacc tcctcggatt
      661 ccattgccca gctatctgtc acttcatcaa aaggacagta gaaaaggaag gtggcaccta
      721 caaatgccat cattgcgata aaggaaaggc tatcgttcaa gatgcctctg ccgacagtgg
      781 tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac
      841 gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg acgcacaatc
      901 ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac
      961 tccggtattt ttacaacaat accacaacaa aacaaacaac aaacaacatt acaatttact
     1021 attctagtcg aaatgggccc cgacatcgtg atgacccaga gccccagcag cctgagcgcc
     1081 agcgtgggcg accgcgtgac catcacctgc cgcagcagca ccggcgccgt gaccaccagc
     1141 aactacgcca gctgggtgca ggagaagccc ggcaagctgt tcaagggcct gatcggcggc
     1201 accaacaacc gcgcccccgg cgtgcccagc cgcttcagcg gcagcctgat cggcgacaag
     1261 gccaccctga ccatcagcag cctgcagccc gaggacttcg ccacctactt ctgcgccctg
     1321 tggtacagca accactgggt gttcggccag ggcaccaagg tggagctgaa gcgcggcggc
     1381 ggcggcagcg gcggcggcgg cagcggcggc ggcggcagca gcggcggcgg cagcgaggtg
     1441 aagctgctgg agagcggcgg cggcctggtg cagcccggcg gcagcctgaa gctgagctgc
     1501 gccgtgagcg gcttcagcct gaccgactac ggcgtgaact gggtgcgcca ggcccccggc
     1561 cgcggcctgg agtggatcgg cgtgatctgg ggcgacggca tcaccgacta caacagcgcc
     1621 ctgaaggacc gcttcatcat cagcaaggac aacggcaaga acaccgtgta cctgcagatg
     1681 agcaaggtgc gcagcgacga caccgccctg tactactgcg tgaccggcct gttcgactac
     1741 tggggccagg gcaccctggt gaccgtgagc agctacccat acgatgttcc agattacgct
     1801 ggtggaggcg gaggttctgg gggaggaggt agtggcggtg gtggttcagg aggcggcgga
     1861 agcggttcgg acccaaaaaa gaagcgtaag gtcgatccca agaagaagag aaaggtagat
     1921 cctaagaaga agagaaaggt agacgcattg gatgacttcg acttagacat gcttgggtca
     1981 gatgctttgg acgactttga cctcgacatg ttgggttctg acgcgctaga tgacttcgac
     2041 ttggacatgt tgggctccga cgctttggac gactttgatc tggacatgtt atgagcttgg
     2101 aatggatctt cgatcccgat cgttcaaaca tttggcaata aagtttctta agattgaatc
     2161 ctgttgccgg tcttgcgacg attatcatat aatttctgtt gaattacgtt aagcatgtaa
     2221 taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc
     2281 aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat
     2341 cgcgcdcggt gtcatctatg ttactagatc gggaattgcc aagctaattc ttgaagacga
     2401 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     2461 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     2521 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatgg
     2581 gaccgactcg cgctgtcatg agacgaattc tgacaggata tattggcggg taaacctaag
     2641 agaaaagagc gtttattaga ataatcggat atttaaaagg gcgtgaaaag gtttatccgt
     2701 tcgtccattt gtatgtgcat gccaaccaca gggttcccct cgggatcaaa gtactttgat
     2761 ccaacccctc cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc cgtcatctga
     2821 aaacgacatg tcgcacaagt cctaagttac gcgacaggct gccgccctgc ccttttcctg
     2881 gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact agaaccggag
     2941 acattacgcc atgaacaaga gcgccgccgc tggcctgctg ggctatgccc gcgtcagcac
     3001 cgacgaccag gacttgacca accaacgggc cgaactgcac gcggccggct gcaccaagct
     3061 gttttccgag aagatcaccg gcaccaggcg cgaccgcccg gagctggcca ggatgcttga
     3121 ccacctacgc cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg cccgcagcac
     3181 ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc tgcgtagcct
     3241 ggcagagccg tgggccgaca ccaccacgcc ggccggccgc atggtgttga ccgtgttcgc
     3301 cggcattgcc gagttcgagc gttccctaat catcgaccgc acccggagcg ggcgcgaggc
     3361 cgccaaggcc cgaggcgtga agtttggccc ccgccctacc ctcaccccgg cacagatcgc
     3421 gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg ctgcactgct
     3481 tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag tgacgcccac
     3541 cgaggccagg cggcgcggtg ccttccgtga ggacgcattg accgaggccg acgccctggc
     3601 ggccgccgag aatgaacgcc aagaggaaca agcatgaaac cgcaccagga cggccaggac
     3661 gaaccgtttt tcattaccga agagatcgag gcggagatga tcgcggccgg gtacgtgttc
     3721 gagccgcccg cgcacctctc aaccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat
     3781 gccaagctgg cggcctggcc ggccagcttg gccgctgaag aaaccgagcg ccgccgtcta
     3841 aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatc
     3901 cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa ggttatcgct gtacttaacc
     3961 agaaaggcgg gtcaggcaag acgaccatcg gaacccatct agcccgcgcc ctgcaactcg
     4021 ccggggccga tgttctgtta gtcgattccg atccccaggg cagtgcccgc gattgggcgg
     4081 ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg
     4141 acgtgaaggc catcggccgg cgcgacttcg tagtgatcga cggagcgccc caggcggcgg
     4201 acttggctgt gtccgcgatc aaggcagccg acttcgtgct gattccggtg cagccaagcc
     4261 cttacgacat atgggccacc gccgacctgg tggagctggt taagcagcgc attgaggtca
     4321 cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg
     4381 gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc cattcttgag tcccgtatca
     4441 cgcagcgcgt gagctaccca ggcactgccg ccgccggcac aaccgttctt gaatcagaac
     4501 ccgagggcga cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa tcaaaactca
     4561 tttgagttaa tgaggtaaag agaaaatgag caaaagcaca aacacgctaa gtgccggccg
     4621 tccgagcgca cgcagcagca aggctgcaac gttggccagc ctggcagaca cgccagccat
     4681 gaagcgggtc aactttcagt tgccggcgga ggatcacacc aagctgaaga tgtacgcggt
     4741 acgccaaggc aagaccatta ccgagctgct atctgaatag atcgcgcagc taccagagta
     4801 aatgagcaaa tgaataaatg agtagatgaa ttttagcggc taaaggaggc ggcatggaaa
     4861 atcaagaaca accaggcacc gacgccgtgg aatgccccat gtgtggagga acgggcggtt
     4921 ggccaggcgt aagcggctgg gttgtctgcc ggccctgcaa tggcactgga acccccaagc
     4981 ccgaggaatc ggcgtgacgg tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg
     5041 ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg cccagcggca acgcatcgag
     5101 gcagaagcac gccccggtga atcgtggcaa gcggccgctg atcgaatccg caaagaatcc
     5161 cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa
     5221 ccagattttt tcgttccgat gctctatgac gtgggcaccc gcgatagtcg cagcatcatg
     5281 gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac
     5341 gagcttccag acgggcacgt agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg
     5401 gattacgacc tggtactgat ggcggtttcc catctaaccg aatccatgaa ccgataccgg
     5461 gaagggaagg gagacaagcc cggccgcgtg ttccgtccac acgttgcgga cgtactcaag
     5521 ttctgccggc gagccgatgg cggaaagcag aaagacgacc tggtagaaac ctgcattcgg
     5581 ttaaacacca cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg
     5641 acggtatccg agggtgaagc cttgattagc cgctacaaga tcgtaaagag cgaaaccggg
     5701 cggccggagt acatcgagat cgagctagct gattggatgt accgcgagat cacagaaggc
     5761 aagaacccgg acgtgctgac ggttcacccc gattactttt tgatcgatcc cggcatcggc
     5821 cgttttctct accgcctggc acgccgcgcc gcaggcaagg cagaagccag atggttgttc
     5881 aagacgatct acgaacgcag tggcagcgcc ggagagttca agaagttctg tttcaccgtg
     5941 cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga ggcggggcag
     6001 gctggcccga tcctagtcat gcgctaccgc aacctgatcg agggcgaagc atccgccggt
     6061 tcctaatgta cggagcagat gctagggcaa attgccctag caggggaaaa aggtcgaaaa
     6121 ggactctttc ctgtggatag cacgtacatt gggaacccaa agccgtacat tgggaaccgg
     6181 aacccgtaca ttgggaaccc aaagccgtac attgggaacc ggtcacacat gtaagtgact
     6241 gatataaaag agaaaaaagg cgatttttcc gcctaaaact ctttaaaact tattaaaact
     6301 cttaaaaccc gcctggcctg tgcataactg tctggccagc gcacagccga agagctgcaa
     6361 aaagcgccta cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc
     6421 gcggccgctg gccgctcaaa aatggctggc ctacggccag gcaatctacc agggcgcgga
     6481 caagccgcgc cgtcgccact cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg
     6541 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggtgacgg tcacagcttg
     6601 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
     6661 gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac
     6721 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
     6781 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg
     6841 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     6901 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     6961 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     7021 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     7081 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     7141 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     7201 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     7261 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     7321 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     7381 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     7441 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     7501 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     7561 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     7621 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggtg attagaaaaa
     7681 ctcatcgagc atcaaatgaa actgcaattt attcatatca ggattatcaa taccatattt
     7741 ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg aggcagttcc ataggatggc
     7801 aagatcctgg tatcggtctg cgattccgac tcgtccaaca tcaatacaac ctattaattt
     7861 cccctcgtca aaaataaggt tatcaagtga gaaatcacca tgagtgacga ctgaatccgg
     7921 tgagaatggc aaaagtttat gcatttcttt ccagacttgt tcaacaggcc agccattacg
     7981 ctcgtcatca aaatcactcg catcaaccaa accgttattc attcgtgatt gcgcctgagc
     8041 gagtcgaaat acgcgatcgc tgttaaaagg acaattacaa acaggaatcg aatgcaaccg
     8101 gcgcaggaac actgccagcg catcaacaat attttcacct gaatcaggat attcttctaa
     8161 tacctggaat gctgttttcc ctgggatcgc agtggtgagt aaccatgcat catcaggagt
     8221 acggataaaa tgcttgatgg tcggaagagg cataaattcc gtcagccagt ttagtctgac
     8281 catctcatct gtaacatcat tggcaacgct acctttgcca tgtttcagaa acaactctgg
     8341 cgcatcgggc ttcccataca atcggtagat tgtcgcacct gattgcccga cattatcgcg
     8401 agcccattta tacccatata aatcagcatc catgttggaa tttaatcgcg gccttgagca
     8461 agacgtttcc cgttgaatat ggctcataac agaacttatt atttccttcc tcttttctac
     8521 agtatttaaa gataccccaa gaagctaatt ataacaagac gaactccaat tcactgttcc
     8581 ttgcattcta aaaccttaaa taccagaaaa cagctttttc aaagttgttt tcaaagttgg
     8641 cgtataacat agtatcgacg gagccgattt tgaaaccgcg gtgatcacag gcagcaacgc
     8701 tctgtcatcg ttacaatcaa catgctaccc tccgcgagat catccgtgtt tcaaacccgg
     8761 cagcttagtt gccgttcttc cgaatagcat cggtaacatg agcaaagtct gccgccttac
     8821 aacggctctc ccgctgacgc cgtcccggac tgatgggctg cctgtatcga gtggtgattt
     8881 tgtgccgagc tgccggtcgg ggagctgttg gctggctggt ggcaggatat attgtggtgt
     8941 aaacataacg aattcgtctc a
//
