LOCUS       GB_UA_F8C              11692 bp    DNA              UNK 01-JAN-1980
DEFINITION  (GB0030,GB_UD_2E3C,GB0036)pDGB3_alpha1
ACCESSION   GB_UA_F8C
VERSION     GB_UA_F8C
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        5..1031
                     /vntifkey="30"
                     /label="35S"
     misc_feature    1033..5091
     terminator      complement(5096..5321)
                     /vntifkey="43"
                     /label="T35S"
     misc_feature    5326..5329
                     /vntifkey="21"
                     /label="GTCA"
     misc_feature    5322..5325
                     /vntifkey="21"
                     /label="CGCT"
     misc_feature    5342..5366
                     /vntifkey="21"
                     /label="RB"
     misc_feature    11651..11676
                     /vntifkey="21"
                     /label="LB"
     CDS             complement(10406..11218)
                     /note="kanamycin resistance gene"
                     /vntifkey="4"
                     /label="KanR"
     rep_origin      complement(9831..10111)
                     /note="pBR322 origin of replication"
                     /vntifkey="33"
                     /label="pBR322\ori"
     misc_feature    complement(9431..9691)
                     /note="bom site from pBR322"
                     /vntifkey="21"
                     /label="pBR322"
     misc_feature    complement(6428..7428)
                     /note="STA region from pVS1 plasmid"
                     /vntifkey="21"
                     /label="STA\region\from\pVS1\plasmid"
     misc_feature    complement(8021..9021)
                     /note="pVS1-REP; replication origin from pVS1"
                     /vntifkey="21"
                     /label="pVS1-Rep\ori"
ORIGIN
        1 ggagactaga gccaagctga tctcctttgc cccggagatc accatggacg actttctcta
       61 tctctacgat ctaggaagaa agttcgacgg agaaggtgac gataccatgt tcaccaccga
      121 taatgagaag attagcctct tcaatttcag aaagaatgct gacccacaga tggttagaga
      181 ggcctacgcg gcaggtctga tcaagacgat ctacccgagt aataatctcc aggagatcaa
      241 ataccttccc aagaaggtta aagatgcagt caaaagattc aggactaact gcatcaagaa
      301 cacagagaaa gatatatttc tcaagatcag aagtactatt ccagtatgga cgattcaagg
      361 cttgcttcat aaaccaaggc aagtaataga gattggagtc tctaagaaag tagttcctac
      421 tgaatcaaag gccatggagt caaaaattca gatcgaggat ctaacagaac tcgccgtgaa
      481 gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga aaatcttcgt
      541 caacatggtg gagcacgaca ctctcgtcta ctccaagaat atcaaagata cagtctcaga
      601 agaccaaagg gctattgaga cttttcaaca aagggtaata tcgggaaacc tcctcggatt
      661 ccattgccca gctatctgtc acttcatcaa aaggacagta gaaaaggaag gtggcaccta
      721 caaatgccat cattgcgata aaggaaaggc tatcgttcaa gatgcctctg ccgacagtgg
      781 tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac
      841 gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg acgcacaatc
      901 ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac
      961 tccggtattt ttacaacaat accacaacaa aacaaacaac aaacaacatt acaatttact
     1021 attctagtcg aaatgacaca gttcgagggc tttaccaacc tgtatcaggt gagcaagaca
     1081 ctgcggtttg agctgatccc acagggcaag accctgaagc acatccagga gcagggcttc
     1141 atcgaggagg acaaggcccg caatgatcac tacaaggagc tgaagcccat catcgatcgg
     1201 atctacaaga cctatgccga ccagtgcctg cagctggtgc agctggattg ggagaacctg
     1261 agcgccgcca tcgactccta tagaaaggag aaaaccgagg agacaaggaa cgccctgatc
     1321 gaggagcagg ccacatatcg caatgccatc cacgactact tcatcggccg gacagacaac
     1381 ctgaccgatg ccatcaataa gagacacgcc gagatctaca agggcctgtt caaggccgag
     1441 ctgtttaatg gcaaggtgct gaagcagctg ggcaccgtga ccacaaccga gcacgagaac
     1501 gccctgctgc ggagcttcga caagtttaca acctacttct ccggctttta tgagaacagg
     1561 aagaacgtgt tcagcgccga ggatatcagc acagccatcc cacaccgcat cgtgcaggac
     1621 aacttcccca agtttaagga gaattgtcac atcttcacac gcctgatcac cgccgtgccc
     1681 agcctgcggg agcactttga gaacgtgaag aaggccatcg gcatcttcgt gagcacctcc
     1741 atcgaggagg tgttttcctt ccctttttat aaccagctgc tgacacagac ccagatcgac
     1801 ctgtataacc agctgctggg aggaatctct cgggaggcag gcaccgagaa gatcaagggc
     1861 ctgaacgagg tgctgaatct ggccatccag aagaatgatg agacagccca catcatcgcc
     1921 tccctgccac acagattcat ccccctgttt aagcagatcc tgtccgatag gaacaccctg
     1981 tctttcatcc tggaggagtt taagagcgac gaggaagtga tccagtcctt ctgcaagtac
     2041 aagacactgc tgagaaacga gaacgtgctg gagacagccg aggccctgtt taacgagctg
     2101 aacagcatcg acctgacaca catcttcatc agccacaaga agctggagac aatcagcagc
     2161 gccctgtgcg accactggga tacactgagg aatgccctgt atgagcggag aatctccgag
     2221 ctgacaggca agatcaccaa gtctgccaag gagaaggtgc agcgcagcct gaagcacgag
     2281 gatatcaacc tgcaggagat catctctgcc gcaggcaagg agctgagcga ggccttcaag
     2341 cagaaaacca gcgagatcct gtcccacgca cacgccgccc tggatcagcc actgcctaca
     2401 accctgaaga agcaggagga gaaggagatc ctgaagtctc agctggacag cctgctgggc
     2461 ctgtaccacc tgctggactg gtttgccgtg gatgagtcca acgaggtgga ccccgagttc
     2521 tctgcccggc tgaccggcat caagctggag atggagcctt ctctgagctt ctacaacaag
     2581 gccagaaatt atgccaccaa gaagccctac tccgtggaga agttcaagct gaactttcag
     2641 atgcctacac tggcctctgg ctgggacgtg aataaggaga agaacaatgg cgccatcctg
     2701 tttgtgaaga acggcctgta ctatctgggc atcatgccaa agcagaaggg caggtataag
     2761 gccctgagct tcgagcccac agagaaaacc agcgagggct ttgataagat gtactatgac
     2821 tacttccctg atgccgccaa gatgatccca aagtgcagca cccagctgaa ggccgtgaca
     2881 gcccactttc agacccacac aacccccatc ctgctgtcca acaatttcat cgagcctctg
     2941 gagatcacaa aggagatcta cgacctgaac aatcctgaga aggagccaaa gaagtttcag
     3001 acagcctacg ccaagaaaac cggcgaccag aagggctaca gagaggccct gtgcaagtgg
     3061 atcgacttca caagggattt tctgtccaag tataccaaga caacctctat cgatctgtct
     3121 agcctgcggc catcctctca gtataaggac ctgggcgagt actatgccga gctgaatccc
     3181 ctgctgtacc acatcagctt ccagagaatc gccgagaagg agatcatgga tgccgtggag
     3241 acaggcaagc tgtacctgtt ccagatctat aacaaggact ttgccaaggg ccaccacggc
     3301 aagcctaatc tgcacacact gtattggacc ggcctgtttt ctccagagaa cctggccaag
     3361 acaagcatca agctgaatgg ccaggccgag ctgttctacc gccctaagtc caggatgaag
     3421 aggatggcac accggctggg agagaagatg ctgaacaaga agctgaagga tcagaaaacc
     3481 ccaatccccg acaccctgta ccaggagctg tacgactatg tgaatcacag actgtcccac
     3541 gacctgtctg atgaggccag ggccctgctg cccaacgtga tcaccaagga ggtgtctcac
     3601 gagatcatca aggataggcg ctttaccagc gacaagttct ttttccacgt gcctatcaca
     3661 ctgaactatc aggccgccaa ttccccatct aagttcaacc agagggtgaa tgcctacctg
     3721 aaggagcacc ccgagacacc tatcatcggc atcgatcggg gcgagagaaa cctgatctat
     3781 atcacagtga tcgactccac cggcaagatc ctggagcagc ggagcctgaa caccatccag
     3841 cagtttgatt accagaagaa gctggacaac agggagaagg agagggtggc agcaaggcag
     3901 gcctggtctg tggtgggcac aatcaaggat ctgaagcagg gctatctgag ccaggtcatc
     3961 cacgagatcg tggacctgat gatccactac caggccgtgg tggtgctgga gaacctgaat
     4021 ttcggcttta agagcaagag gaccggcatc gccgagaagg ccgtgtacca gcagttcgag
     4081 aagatgctga tcgataagct gaattgcctg gtgctgaagg actatccagc agagaaagtg
     4141 ggaggcgtgc tgaacccata ccagctgaca gaccagttca cctcctttgc caagatgggc
     4201 acccagtctg gcttcctgtt ttacgtgcct gccccatata catctaagat cgatcccctg
     4261 accggcttcg tggacccctt cgtgtggaaa accatcaaga atcacgagag ccgcaagcac
     4321 ttcctggagg gcttcgactt tctgcactac gacgtgaaaa ccggcgactt catcctgcac
     4381 tttaagatga acagaaatct gtccttccag aggggcctgc ccggctttat gcctgcatgg
     4441 gatatcgtgt tcgagaagaa cgagacacag tttgacgcca agggcacccc tttcatcgcc
     4501 ggcaagagaa tcgtgccagt gatcgagaat cacagattca ccggcagata ccgggacctg
     4561 tatcctgcca acgagctgat cgccctgctg gaggagaagg gcatcgtgtt cagggatggc
     4621 tccaacatcc tgccaaagct gctggagaat gacgattctc acgccatcga caccatggtg
     4681 gccctgatcc gcagcgtgct gcagatgcgg aactccaatg ccgccacagg cgaggactat
     4741 atcaacagcc ccgtgcgcga tctgaatggc gtgtgcttcg actcccggtt tcagaaccca
     4801 gagtggccca tggacgccga tgccaatggc gcctaccaca tcgccctgaa gggccagctg
     4861 ctgctgaatc acctgaagga gagcaaggat ctgaagctgc agaacggcat ctccaatcag
     4921 gactggctgg cctacatcca ggagctgcgc aacaaaaggc cggcggccac gaaaaaggcc
     4981 ggccaggcaa aaaagaaaaa gggatcctac ccatacgatg ttccagatta cgcttatccc
     5041 tacgacgtgc ctgattatgc atacccatat gatgtccccg actatgccta agcttcggcc
     5101 atgctagagt ccgcaaaaat caccagtctc tctctacaaa tctatctctc tctatttttc
     5161 tccagaataa tgtgtgagta gttcccagat aagggaatta gggttcttat agggtttcgc
     5221 tcatgtgttg agcatataag aaacccttag tatgtatttg tatttgtaaa atacttctat
     5281 caataaaatt tctaattcct aaaaccaaaa tccagtgacc tcgctgtcat gagacgaatt
     5341 ctgacaggat atattggcgg gtaaacctaa gagaaaagag cgtttattag aataatcgga
     5401 tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca tgccaaccac
     5461 agggttcccc tcgggatcaa agtactttga tccaacccct ccgctgctat agtgcagtcg
     5521 gcttctgacg ttcagtgcag ccgtcatctg aaaacgacat gtcgcacaag tcctaagtta
     5581 cgcgacaggc tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca
     5641 taaagtagaa tacttgcgac tagaaccgga gacattacgc catgaacaag agcgccgccg
     5701 ctggcctgct gggctatgcc cgcgtcagca ccgacgacca ggacttgacc aaccaacggg
     5761 ccgaactgca cgcggccggc tgcaccaagc tgttttccga gaagatcacc ggcaccaggc
     5821 gcgaccgccc ggagctggcc aggatgcttg accacctacg ccctggcgac gttgtgacag
     5881 tgaccaggct agaccgcctg gcccgcagca cccgcgacct actggacatt gccgagcgca
     5941 tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac accaccacgc
     6001 cggccggccg catggtgttg accgtgttcg ccggcattgc cgagttcgag cgttccctaa
     6061 tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc
     6121 cccgccctac cctcaccccg gcacagatcg cgcacgcccg cgagctgatc gaccaggaag
     6181 gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg
     6241 cacttgagcg cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt gccttccgtg
     6301 aggacgcatt gaccgaggcc gacgccctgg cggccgccga gaatgaacgc caagaggaac
     6361 aagcatgaaa ccgcaccagg acggccagga cgaaccgttt ttcattaccg aagagatcga
     6421 ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc gcgcacctct caaccgtgcg
     6481 gctgcatgaa atcctggccg gtttgtctga tgccaagctg gcggcctggc cggccagctt
     6541 ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag
     6601 cttgcgtcat gcggtcgctg cgtatatgat ccgatgagta aataaacaaa tacgcaaggg
     6661 gaacgcatga aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc
     6721 ggaacccatc tagcccgcgc cctgcaactc gccggggccg atgttctgtt agtcgattcc
     6781 gatccccagg gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt
     6841 gtcggcatcg accgcccgac gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc
     6901 gtagtgatcg acggagcgcc ccaggcggcg gacttggctg tgtccgcgat caaggcagcc
     6961 gacttcgtgc tgattccggt gcagccaagc ccttacgaca tatgggccac cgccgacctg
     7021 gtggagctgg ttaagcagcg cattgaggtc acggatggaa ggctacaagc ggcctttgtc
     7081 gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg
     7141 tacgagctgc ccattcttga gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc
     7201 gccgccggca caaccgttct tgaatcagaa cccgagggcg acgctgcccg cgaggtccag
     7261 gcgctggccg ctgaaattaa atcaaaactc atttgagtta atgaggtaaa gagaaaatga
     7321 gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa
     7381 cgttggccag cctggcagac acgccagcca tgaagcgggt caactttcag ttgccggcgg
     7441 aggatcacac caagctgaag atgtacgcgg tacgccaagg caagaccatt accgagctgc
     7501 tatctgaata gatcgcgcag ctaccagagt aaatgagcaa atgaataaat gagtagatga
     7561 attttagcgg ctaaaggagg cggcatggaa aatcaagaac aaccaggcac cgacgccgtg
     7621 gaatgcccca tgtgtggagg aacgggcggt tggccaggcg taagcggctg ggttgtctgc
     7681 cggccctgca atggcactgg aacccccaag cccgaggaat cggcgtgacg gtcgcaaacc
     7741 atccggcccg gtacaaatcg gcgcggcgct gggtgatgac ctggtggaga agttgaaggc
     7801 cgcgcaggcc gcccagcggc aacgcatcga ggcagaagca cgccccggtg aatcgtggca
     7861 agcggccgct gatcgaatcc gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc
     7921 gattaggaag ccgcccaagg gcgacgagca accagatttt ttcgttccga tgctctatga
     7981 cgtgggcacc cgcgatagtc gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg
     8041 tgaccgacga gctggcgagg tgatccgcta cgagcttcca gacgggcacg tagaggtttc
     8101 cgcagggccg gccggcatgg ccagtgtgtg ggattacgac ctggtactga tggcggtttc
     8161 ccatctaacc gaatccatga accgataccg ggaagggaag ggagacaagc ccggccgcgt
     8221 gttccgtcca cacgttgcgg acgtactcaa gttctgccgg cgagccgatg gcggaaagca
     8281 gaaagacgac ctggtagaaa cctgcattcg gttaaacacc acgcacgttg ccatgcagcg
     8341 tacgaagaag gccaagaacg gccgcctggt gacggtatcc gagggtgaag ccttgattag
     8401 ccgctacaag atcgtaaaga gcgaaaccgg gcggccggag tacatcgaga tcgagctagc
     8461 tgattggatg taccgcgaga tcacagaagg caagaacccg gacgtgctga cggttcaccc
     8521 cgattacttt ttgatcgatc ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc
     8581 cgcaggcaag gcagaagcca gatggttgtt caagacgatc tacgaacgca gtggcagcgc
     8641 cggagagttc aagaagttct gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc
     8701 ggagtacgat ttgaaggagg aggcggggca ggctggcccg atcctagtca tgcgctaccg
     8761 caacctgatc gagggcgaag catccgccgg ttcctaatgt acggagcaga tgctagggca
     8821 aattgcccta gcaggggaaa aaggtcgaaa aggactcttt cctgtggata gcacgtacat
     8881 tgggaaccca aagccgtaca ttgggaaccg gaacccgtac attgggaacc caaagccgta
     8941 cattgggaac cggtcacaca tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc
     9001 cgcctaaaac tctttaaaac ttattaaaac tcttaaaacc cgcctggcct gtgcataact
     9061 gtctggccag cgcacagccg aagagctgca aaaagcgcct acccttcggt cgctgcgctc
     9121 cctacgcccc gccgcttcgc gtcggcctat cgcggccgct ggccgctcaa aaatggctgg
     9181 cctacggcca ggcaatctac cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg
     9241 gcgcccacat caaggcaccc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac
     9301 acatgcagct cccggtgacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     9361 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac
     9421 gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag
     9481 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
     9541 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     9601 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     9661 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     9721 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     9781 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     9841 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     9901 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     9961 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
    10021 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
    10081 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
    10141 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
    10201 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
    10261 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
    10321 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
    10381 tttggtcatg cattctaggt gattagaaaa actcatcgag catcaaatga aactgcaatt
    10441 tattcatatc aggattatca ataccatatt tttgaaaaag ccgtttctgt aatgaaggag
    10501 aaaactcacc gaggcagttc cataggatgg caagatcctg gtatcggtct gcgattccga
    10561 ctcgtccaac atcaatacaa cctattaatt tcccctcgtc aaaaataagg ttatcaagtg
    10621 agaaatcacc atgagtgacg actgaatccg gtgagaatgg caaaagttta tgcatttctt
    10681 tccagacttg ttcaacaggc cagccattac gctcgtcatc aaaatcactc gcatcaacca
    10741 aaccgttatt cattcgtgat tgcgcctgag cgagtcgaaa tacgcgatcg ctgttaaaag
    10801 gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa
    10861 tattttcacc tgaatcagga tattcttcta atacctggaa tgctgttttc cctgggatcg
    10921 cagtggtgag taaccatgca tcatcaggag tacggataaa atgcttgatg gtcggaagag
    10981 gcataaattc cgtcagccag tttagtctga ccatctcatc tgtaacatca ttggcaacgc
    11041 tacctttgcc atgtttcaga aacaactctg gcgcatcggg cttcccatac aatcggtaga
    11101 ttgtcgcacc tgattgcccg acattatcgc gagcccattt atacccatat aaatcagcat
    11161 ccatgttgga atttaatcgc ggccttgagc aagacgtttc ccgttgaata tggctcataa
    11221 cagaacttat tatttccttc ctcttttcta cagtatttaa agatacccca agaagctaat
    11281 tataacaaga cgaactccaa ttcactgttc cttgcattct aaaaccttaa ataccagaaa
    11341 acagcttttt caaagttgtt ttcaaagttg gcgtataaca tagtatcgac ggagccgatt
    11401 ttgaaaccgc ggtgatcaca ggcagcaacg ctctgtcatc gttacaatca acatgctacc
    11461 ctccgcgaga tcatccgtgt ttcaaacccg gcagcttagt tgccgttctt ccgaatagca
    11521 tcggtaacat gagcaaagtc tgccgcctta caacggctct cccgctgacg ccgtcccgga
    11581 ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt
    11641 ggctggctgg tggcaggata tattgtggtg taaacataac gaattcgtct ca
//
