LOCUS       GB0226        4011 bp    DNA     circular     24-FEB-2013
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|654613836|
COMMENT     VNTDBDATE|654613836|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pEGB Tnos:NptII:Pnos (pDGB1_alpha1R)|
COMMENT     VNTAUTHORNAME|Marta Vazquez|
FEATURES             Location/Qualifiers
     mutation        complement(217..217)
                     /vntifkey="62"
                     /label=G>C
     mutation        complement(349..349)
                     /vntifkey="62"
                     /label=T>A
     mutation        complement(541..541)
                     /vntifkey="62"
                     /label=T>C
     mutation        complement(694..694)
                     /vntifkey="62"
                     /label=T>C
     promoter        complement(1111..1401)
                     /vntifkey="30"
                     /label=Pnos
     terminator      complement(4..318)
                     /vntifkey="43"
                     /label=Tnos
     CDS             complement(319..1110)
                     /vntifkey="4"
                     /label=NptII
     rep_origin      complement(1472..2196)
                     /vntifkey="33"
                     /label=pUC-ori
     repeat_region   3972..3995
                     /vntifkey="34"
                     /label=LB
     rep_origin      3397..3955
                     /vntifkey="33"
                     /label=pSa
     CDS             2292..3115
                     /vntifkey="4"
                     /label=KanR
     misc_feature    1406..1409
                     /vntifkey="21"
                     /label=GTCA
     repeat_region   1422..1446
                     /vntifkey="34"
                     /label=RB
     misc_feature    complement(1402..1405)
                     /vntifkey="21"
                     /label=GGAG
BASE COUNT     1053 a      1025 c       945 g       988 t 
ORIGIN
        1 agcgatcagc ttgcatgccg gtcgatctag taacatagat gacaccgcgc gcgataattt 
       61 atcctagttt gcgcgctata ttttgttttc tatcgcgtat taaatgtata attgcgggac 
      121 tctaatcata aaaacccatc tcataaataa cgtcatgcat tacatgttaa ttattacatg 
      181 cttaacgtaa ttcaacagaa attatatgat aatcatggca agaccggcaa caggattcaa 
      241 tcttaagaaa ctttattgcc aaatgtttga acgatctgct tgactctagc tagagtccga 
      301 accccagagt cccgctcaga agaactcgtc aagaaggcga tagaaggcta tgcgctgcga 
      361 atcgggagcg gcgataccgt aaagcacgag gaagcggtca gcccattcgc cgccaagctc 
      421 ttcagcaata tcacgggtag ccaacgctat gtcctgatag cggtccgcca cacccagccg 
      481 gccacagtcg atgaatccag aaaagcggcc attttccacc atgatattcg gcaagcaggc 
      541 gtcgccgtgg gtcacgacga gatcctcgcc gtcgggcatc cgcgccttga gcctggcgaa 
      601 cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat cgacaagacc 
      661 ggcttccatc cgagtacgtg ctcgctcgat gcggtgtttc gcttggtggt cgaatgggca 
      721 ggtagccgga tcaagcgtat gcagccgccg cattgcatca gccatgatgg atactttctc 
      781 ggcaggagca aggtgagatg acaggagatc ctgccccggc acttcgccca atagcagcca 
      841 gtcccttccc gcttcagtga caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc 
      901 cagccacgat agccgcgctg cctcgtcttg gagttcattc agggcaccgg acaggtcggt 
      961 cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg aacacggcgg catcagagca 
     1021 gccgattgtc tgttgtgccc agtcatagcc gaatagcctc tccacccaag cggccggaga 
     1081 acctgcgtgc aatccatctt gttcaatcat gcctcgatcg agttgagagt gaatatgaga 
     1141 ctctaattgg ataccgaggg gaatttatgg aacgtcagtg gagcattttt gacaagaaat 
     1201 atttgctagc tgatagtgac cttaggcgac ttttgaacgc gcaataatgg tttctgacgt 
     1261 atgtgcttag ctcattaaac tccagaaacc cgcggctgag tggctccttc aacgttgcgg 
     1321 ttctgtcagt tccaaacgta aaacggcttg tcccgcgtca tcggcggggg tcataacgtg 
     1381 actcccttaa ttctcatgta tctccgtcat gagacggatc ctgacaggat atattggcgg 
     1441 gtaaactaag tcgctgtatg tgtttgtttg agatctcatg tgagcaaaag gccagcaaaa 
     1501 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 
     1561 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 
     1621 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 
     1681 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 
     1741 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 
     1801 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 
     1861 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 
     1921 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac 
     1981 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaagaagag ttggtagctc 
     2041 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 
     2101 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 
     2161 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 
     2221 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgtgta 
     2281 acattggtct agtgattaga aaaactcatc gagcatcaaa tgaaactgca atttattcat 
     2341 atcaggatta tcaataccat atttttgaaa aagccgtttc tgtaatgaag gagaaaactc 
     2401 accgaggcag ttccatagga tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc 
     2461 aacatcaata caacctatta atttcccctc gtcaaaaata aggttatcaa gtgagaaatc 
     2521 accatgagtg acgactgaat ccggtgagaa tggcaaaagt ttatgcattt ctttccagac 
     2581 ttgttcaaca ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt 
     2641 attcattcgt gattgcgcct gagcgagtcg aaatacgcga tcgctgttaa aaggacaatt 
     2701 acaaacagga atcgaatgca accggcgcag gaacactgcc agcgcatcaa caatattttc 
     2761 acctgaatca ggatattctt ctaatacctg gaatgctgtt ttccctggga tcgcagtggt 
     2821 gagtaaccat gcatcatcag gagtacggat aaaatgcttg atggtcggaa gaggcataaa 
     2881 ttccgtcagc cagtttagtc tgaccatctc atctgtaaca acattggcaa cgctaccttt 
     2941 gccatgtttc agaaacaact ctggcgcatc gggcttccca tacaatcggt agattgtcgc 
     3001 acctgattgc ccgacattat cgcgagccca tttataccca tataaatcag catccatgtt 
     3061 ggaatttaat cgcggccttg agcaagacgt ttcccgttga atatggctca taacacccct 
     3121 tgtattactg tttatgtaag cagacagttt tattgttcat gatgatatat ttttatcttg 
     3181 tgcaatgtaa catcagagat tttgagacac aacgtggctt tgttgaataa atcgaacttt 
     3241 tgctgagttg aaggatcaga tcacgcatct tcccgacaac gcagaccgtt ccgtggcaaa 
     3301 gcaaaagttc aaaatcacca actggtccac ctacaacaaa gctctcatca accgtggctc 
     3361 cctcactttc tggctggatg atggggcgat tcaggcgatc cccatccaac agcccgccgt 
     3421 cgagcgggct tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc 
     3481 gctaatgccc cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca 
     3541 cgtgaaatcg ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata 
     3601 aggtcacgtg aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc 
     3661 aacagcttgc aaacacccct cgctccggca agtagttaca gcaagtagta tgttcaatta 
     3721 gcttttcaat tatgaatata tatatcaatt attggtcgcc cttggcttgt ggacaatgcg 
     3781 ctacgcgcac cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt cgagcgccag 
     3841 cgcctttgcc cacaacccgg cggccggccg caacagatcg ttttataaat tttttttttt 
     3901 gaaaaagaaa aagcccgaaa ggcggcaacc tctcgggctt ctggatttcc gatccccgga 
     3961 attagagatc ttggcaggat atattgtggt gtaacggatc cgtctcagga g 
//
