LOCUS       GB01041     7196 bp ds-DNA     circular     23-JUN-2015
DEFINITION  .
FEATURES             Location/Qualifiers
     CDS             complement(5444..6304)
                     /label="AmpR"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    5585..5585
                     /label="A>C"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    6700..6700
                     /label="A>C"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    4122..4142
                     /label="NLS"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             6..4142
                     /label="mCas9"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
ORIGIN
        1 ctcgaatgga caagaagtac tccattgggc tcgctatcgg cacaaacagc gtcggctggg
       61 ccgtcattac ggccgagtac aaggtgccga gcaaaaaatt caaagttctg ggcaataccg
      121 atcgccacag cataaagaag aacctcattg gcgccctcct gttcgactcc ggggaaacgg
      181 acgaagccac gcggctcaaa agaacagcac ggcgcagata tacccgcaga aagaatcgga
      241 tctgctacct gcaggagatc tttagtaatg agatggctaa ggtggatgac tctttcttcc
      301 ataggctgga ggagtccttt ttggtggagg aggataaaaa gcacgagcgc cacccaatct
      361 ttggcaatat cgtggacgag gtggcgtacc atgaaaagta cccaaccata tatcatctga
      421 ggaagaagct tgtagacagt actgataagg ctgacttgcg gttgatctat ctcgcgctgg
      481 cgcatatgat caaatttcgg ggacacttcc tcatcgaggg ggacctgaac ccagacaaca
      541 gcgatgtcga caaactcttt atccaactgg ttcagactta caatcagctt ttcgaagaga
      601 acccgatcaa cgcatccgga gttgacgcca aagcaatcct gagcgctagg ctgtccaaat
      661 cccggcggct cgaaaacctc atcgcacagc tccctgggga gaagaagaac ggcctgtttg
      721 gtaatcttat cgccctgtca ctcgggctga cccccaactt taaatctaac ttcgacctgg
      781 ccgaagatgc caagcttcaa ctgagcaaag acacctacga tgatgatctc gacaatctgc
      841 tggcccagat cggcgaccag tacgcagacc tttttttggc ggcaaagaac ctgtcagacg
      901 ccattctgct gagtgatatt ctgcgagtga acacggagat caccaaagct ccgctgagcg
      961 ctagtatgat caagcgctat gatgagcacc accaagactt gactttgctg aaggcccttg
     1021 tcagacagca actgcctgag aagtacaagg aaattttctt cgatcagtct aaaaatggct
     1081 acgccggata cattgacggc ggagcaagcc aggaggaatt ttacaaattt attaagccca
     1141 tcttggaaaa aatggacggc accgaggagc tgctggtaaa gcttaacaga gaagatctgt
     1201 tgcgcaaaca gcgcactttc gacaatggaa gcatccccca ccagattcac ctgggcgaac
     1261 tgcacgctat cctcaggcgg caagaggatt tctacccctt tttgaaagat aacagggaaa
     1321 agattgagaa aatcctcaca tttcggatac cctactatgt aggccccctc gcccggggaa
     1381 attccagatt cgcgtggatg actcgcaaat cagaagagac tatcactccc tggaacttcg
     1441 aggaagtcgt ggataagggg gcctctgccc agtccttcat cgaaaggatg actaactttg
     1501 ataaaaatct gcctaacgaa aaggtgcttc ctaaacactc tctgctgtac gagtacttca
     1561 cagtttataa cgagctcacc aaggtcaaat acgtcacaga agggatgaga aagccagcat
     1621 tcctgtctgg agagcagaag aaagctatcg tggacctcct cttcaagacg aaccggaaag
     1681 ttaccgtgaa acagctcaaa gaagattatt tcaaaaagat tgaatgtttc gactctgttg
     1741 aaatcagcgg agtggaggat cgcttcaacg catccctggg aacgtatcac gatctcctga
     1801 aaatcattaa agacaaggac ttcctggaca atgaggagaa cgaggacatt cttgaggaca
     1861 ttgtcctcac ccttacgttg tttgaagata gggagatgat tgaagaacgc ttgaaaactt
     1921 acgctcatct cttcgacgac aaagtcatga aacagctcaa gaggcgccga tatacaggat
     1981 gggggcggct gtcaagaaaa ctgatcaatg ggatccgaga caagcagagt ggaaagacaa
     2041 tcctggattt tcttaagtcc gatggatttg ccaaccggaa cttcatgcag ttgatccatg
     2101 atgactctct cacctttaag gaggacatcc agaaagcaca agtttctggc cagggggaca
     2161 gtctccacga gcacatcgct aatcttgcag gtagcccagc tatcaaaaag ggaatactgc
     2221 agaccgttaa ggtcgtggat gaactcgtca aagtaatggg aaggcataag cccgagaata
     2281 tcgttatcga gatggcccga gagaaccaaa ctacccagaa gggacagaag aacagtaggg
     2341 aaaggatgaa gaggattgaa gagggtataa aagaactggg gtcccaaatc cttaaggaac
     2401 acccagttga aaacacccag cttcagaatg agaagctcta cctgtactac ctgcagaacg
     2461 gcagggacat gtacgtggat caggaactgg acatcaatcg gctctccgac tacgacgtgg
     2521 atgccatcgt gccccagtct tttctcaaag atgattctat tgataataaa gtgttgacaa
     2581 gatccgataa aaatagaggg aagagtgata acgtcccctc agaagaagtt gtcaagaaaa
     2641 tgaaaaatta ttggcggcag ctgctgaacg ccaaactgat cacacaacgg aagttcgata
     2701 atctgactaa ggctgaacga ggtggcctgt ctgagttgga taaagccggc ttcatcaaaa
     2761 ggcagcttgt tgagacacgc cagatcacca agcacgtggc ccaaattctc gattcacgca
     2821 tgaacaccaa gtacgatgaa aatgacaaac tgattcgaga ggtgaaagtt attactctga
     2881 agtctaagct ggtttcagat ttcagaaagg actttcagtt ttataaggtg agagagatca
     2941 acaattacca ccatgcgcat gatgcctacc tgaatgcagt ggtaggcact gcacttatca
     3001 aaaaatatcc caagcttgaa tctgaatttg tttacggaga ctataaagtg tacgatgtta
     3061 ggaaaatgat cgcaaagtct gagcaggaaa taggcaaggc caccgctaag tacttctttt
     3121 acagcaatat tatgaatttt ttcaagaccg agattacact ggccaatgga gagattcgga
     3181 agcgaccact tatcgaaaca aacggagaaa caggagaaat cgtgtgggac aagggtaggg
     3241 atttcgcgac agtccggaag gtcctgtcca tgccgcaggt gaacatcgtt aaaaagaccg
     3301 aagtacagac cggaggcttc tccaaggaaa gtatcctccc gaaaaggaac agcgacaagc
     3361 tgatcgcacg caaaaaagat tgggacccca agaaatacgg cggattcgat tctcctacag
     3421 tcgcttacag tgtactggtt gtggccaaag tggagaaagg gaagtctaaa aaactcaaaa
     3481 gcgtcaagga actgctgggc atcacaatca tggagcgatc aagcttcgaa aaaaacccca
     3541 tcgactttct cgaggcgaaa ggatataaag aggtcaaaaa agacctcatc attaagcttc
     3601 ccaagtactc tctctttgag cttgaaaacg gccggaaacg aatgctcgct agtgcgggcg
     3661 agctgcagaa aggtaacgag ctggcactgc cctctaaata cgttaatttc ttgtatctgg
     3721 ccagccacta tgaaaagctc aaaggatctc ccgaagataa tgagcagaag cagctgttcg
     3781 tggaacaaca caaacactac cttgatgaga tcatcgagca aataagcgaa ttctccaaaa
     3841 gagtgatcct cgccgacgct aacctcgata aggtgctttc tgcttacaat aagcacaggg
     3901 ataagcccat cagggagcag gcagaaaaca ttatccactt gtttactctg accaacttgg
     3961 gcgcgcctgc agccttcaag tacttcgaca ccaccataga cagaaagcgg tacacctcta
     4021 caaaggaggt cctggacgcc acactgattc atcagtcaat tacggggctc tatgaaacaa
     4081 gaatcgacct ctctcagctc ggtggagaca gcagggctga ccccaagaag aagaggaagg
     4141 tgtgagcttc gagaccactc atcgcccatc actagtgaat tcgcggccgc ctgcaggtcg
     4201 accatatggg agagctccca acgcgttgga tgcatagctt gagtattcta tagtgtcacc
     4261 taaatagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     4321 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     4381 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     4441 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     4501 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     4561 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     4621 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     4681 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     4741 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     4801 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     4861 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     4921 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     4981 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     5041 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     5101 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     5161 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     5221 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     5281 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     5341 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     5401 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     5461 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     5521 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     5581 cgcgcgaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     5641 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     5701 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     5761 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     5821 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     5881 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     5941 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     6001 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     6061 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     6121 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     6181 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     6241 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     6301 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     6361 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     6421 gaaaagtgcc acctgatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     6481 atcaggaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     6541 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     6601 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     6661 actccaacgt caaagggcga aaaaccgtct atcagggcgc tggcccacta cgtgaaccat
     6721 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     6781 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     6841 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     6901 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc cattcaggct
     6961 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     7021 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     7081 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tgggcccgac
     7141 gtcgcatgct cccggccgcc atggcggccg cgggaattcg atgggcgatg agtggt
//