Recommended TUs for genome editing
The table below displays the TUs/modules recommended for combining with the gRNA constructs for gene editing
Recommended Cas9 TUs / modules
|
Name |
Description |
Vector |
| GB0639 |
pEGB 35s:hCas9:tNos |
TU for the expression of the human codon optimized Cas9 |
pDGB2_alpha2 |
| GB2234 |
pEGB tNos:nptII:PNos-35s:Cas9:tNos-35s:DsRed:tNos |
Module for the constitutive expression of the nptII, Cas9 and DsRed genes |
pDGB3_alpha1 |
| GB2235 |
pEGB tNos:nptII:PNos-35s:Cas9:tNos-35s:DsRed:tNos |
Module for the constitutive expression of the nptII, Cas9 and DsRed genes |
pDGB3_omega1 |
Recommended Cas12a TUs / modules
|
Name |
Description |
Vector |
| GB1440 |
pEGB 35s:AsCas12a:t35s |
TU for the constitutive expression of the human codon optimized Cas12a from Acidominococcus sp. |
pDGB3_alpha1 |
| GB1441 |
pEGB 35s:LbCas12a:t35s |
TU for the constitutive expression of the human codon optimized Cas12a from Lachnospiraceae sp. |
pDGB3_alpha1 |
| GB2479 |
pEGB pAtUBQ10:LbCas12a:Tnos |
TU for the constitutive expression of the human codon optimized Cas12a from Lachnospiraceae sp. under the Arabidopsis ubiquitin promoter. |
pDGB3_omega1 |
