Recommended TUs for gene regulation
The table below displays the TUs/modules recommended for combining with the gRNA constructs for gene regulation
Recommended dCas9 TUs for gene activation
|
Name |
Description |
Vector |
| GB1189 |
pEGB 35s:dCas9-VP64:tNOS |
TU for the expression of the dCas9 with the activation domain VP64 as a CT fusion |
pDGB3_alpha2 |
| GB1190 |
pEGB 35s:dCas9-EDLL:tNOS |
TU for the expression of the dCas9 with the activation domain EDLL as a CT fusion |
pDGB3_alpha2 |
| GB1794 |
3alpha2_35s-dCas9:P300_Tnos |
TU for the constitutive expression of the dCas9 fused to the transcriptional activator P300 |
pDGB3_alpha2 |
| GB1851 |
3alpha2_35s-dCas9:VP192-Tnos |
TU for the constitutive expression of the dCas9 fused to the transcriptional activator VP192 |
pDGB3_alpha2 |
| GB1826 |
3alpha2_35s-dCas9:VPR-Tnos |
TU for the constitutive expression of dCas9 fused to VPR (VP64-p65-Rta) activation domains |
pDGB3_alpha2 |
| GB1824 |
3alpha2_35s-dCas9:ERF2-Tnos |
TU for the constitutive expression of dCas9 fused to the transcriptional activation domain ERF2 |
pDGB3_alpha2 |
| GB2047 |
3alpha2_35s-dCas9:TV-Tnos |
TU for the constitutive expression of dCas9 fused to TV (TALx6 - VP128) activation domains |
pDGB3_alpha2 |
Recommended TUs for gene activation in combination with dCas9 TUs when using a modified scaffold
|
Name |
Description |
Vector |
Modified scaffold |
| GB1403 |
pEGB3alpha1 35S:MS2-VP64:Tnos |
TU for the constitutive expression of the MS2 coat protein fused to the transcriptional activator VP64 |
pDGB3_alpha1 |
scRNA (GB1437), scRNA (GB1436), scRNA (GB2461) |
| GB1738 |
pDGB3_alpha2: 35s_MS2:EDLL_Tnos |
TU for the constitutive expression of the MS2 coat protein fused on Ct to the activation domain EDLL |
pDGB3_alpha2 |
scRNA2.1 |
| GB1852 |
3alpha2_35s-Ms2:VP192_Tnos |
TU for the constitutive expression of the MS2 coat protein fused to the transcriptional activator VP192 |
pDGB3_alpha2 |
scRNA2.1 |
| GB1830 |
3alpha2_35s-MS2:VPR-Tnos |
TU for the constitutive expression of the MS2 coat protein fused on Ct to the activation domain VPR (VP64+p65+Rta) |
pDGB3_alpha2 |
scRNA2.1 |
| GB1833 |
3alpha2_35s-MS2:ERF2-Tnos |
TU for the constitutive expression of the MS2 coat protein fused on Ct to the transactivation domain of ERF2 |
pDGB3_alpha2 |
scRNA2.1 |
| GB2048 |
3alpha2_35s-MS2:TV-Tnos |
TU for the constitutive expression of Ms2 fused to TV (TALx6 - VP128) activation domains |
pDGB3_alpha2 |
scRNA2.1 |
| GB2085 |
pDGB3_Omega1_35s-Ms2:VPR-Tnos-35s-dCas9:EDLL-Tnos |
Module for the expression of Ms2 protein fused to VPR and dCas9 fused to EDLL |
pDGB3_omega1 |
scRNA2.1 |
| GB1460 |
pEGB3alpha1 35S:NLS-PP7-VP64:Tnos |
TU for the constitutive expression of the PP7 coat protein fused on Ct to the activation domain VP64 |
pDGB3_alpha1 |
PP7 (GB1450) |
| GB1476 |
35s:COM-VP64:Tnos |
TU for the expression of RNA-binding protein COM fused to the VP64 activation domain |
pDGB3_alpha1 |
COM (GB1451) |
Recommended TUs for gene activation - SunTag strategy
|
Name |
Description |
Vector |
| GB1603 |
35s:dCas9-SunTag:Tnos |
TU for the expression of the dCas9 fused to the SunTag epitope |
pDGB3_alpha2 |
| GB1592 |
35S:scFv-VP64:Tnos |
TU for the expression of the scFv (recognizes the SunTag epitope) fused to the VP64 domain |
pDGB3_alpha1 |
| GB1836 |
3alpha2_35s-scFv-EDLL-tNOS |
TU for constitutive expression of ScFv antibody fused to EDLL activation domain |
pDGB3_alpha2 |
Recommended TUs for gene repression
|
Name |
Description |
Vector |
| GB1172 |
pEGB 35s:dCas9-BRD:tNOS |
TU for the expression of the dCas9 with the repressor domain BRD as a CT fusion |
pDGB3_alpha2 |
| GB1188 |
pEGB 35s:dCas9:SRDX:tNOS |
TU for the expression of the dCas9 with the repressor domain SRDX as a CT fusion |
pDGB3_alpha2 |
| GB1191 |
pEGB 35s:dCas9:TNos |
TU for the constitutive expression of the human dCas9 (D10A, H840A; dead Cas9) |
pDGB3_alpha2 |
