Go to: GB Domesticator / Domestication - Phylogeny Search
Domestication - Synthetic Strategy
Take a look to the IMPORTANT NOTICE regarding the changes on the domestication tools.
The Synthetic Strategy generates a domesticated sequence for gene synthesis. By synthesizing your sequence, you can skip the mutagenesis PCR step and go directly to the BsmBI restriction ligation reaction that brings together the part and the pUPD vector.