GB_UA_257A
35s:COM-VP64:Tnos (TU)
Description: | GB1476. TU for the expression of RNA-binding protein COM fused to the VP64 activation domain. Author: JB. |
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Reference: | Zalatan, J. G., Lee, M. E., Almeida, R., Gilbert, L. A., Whitehead, E. H., La Russa, M., ... & Lim, W. A. (2015). Engineering complex synthetic transcriptional programs with CRISPR RNA scaffolds. Cell, 160(1-2), 339-350. |
General Information | |
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ID | GB_UA_257A |
Name | 35s:COM-VP64:Tnos |
Function | TU |
Category | TU |
Level | 1-alpha |
Vector | pDGB3_alpha1 |
Prefix | GGAG |
Suffix | GTCA |
Enzyme out | BsmBI |
Resistance | Kanamycin |
Assembly Standard | 3.0 |
Owner | |
Public | True |
Genbank_file | GB_UA_257A |
SBOL_file | GB_UA_257A |
View GenBank Features |
Component Elements | ||
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ID | Name | Type |
GB0030 | pP35S | PROM+UTR+ATG |
GB_UD_4952 | pUPD2:NLS-COM1 (B3-B4) | CDS |
GB01186 | 3xNLS-VP64 AD | CT |
GB0037 | pTnos | TER |
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