GB_UA_257A
35s:COM-VP64:Tnos (TU)
| Description: | GB1476. TU for the expression of RNA-binding protein COM fused to the VP64 activation domain. Author: JB. |
|---|---|
| Reference: | Zalatan, J. G., Lee, M. E., Almeida, R., Gilbert, L. A., Whitehead, E. H., La Russa, M., ... & Lim, W. A. (2015). Engineering complex synthetic transcriptional programs with CRISPR RNA scaffolds. Cell, 160(1-2), 339-350. |
| General Information | |
|---|---|
| ID | GB_UA_257A |
| Name | 35s:COM-VP64:Tnos |
| Function | TU |
| Category | TU |
| Level | 1-alpha |
| Vector | pDGB3_alpha1 |
| Prefix | GGAG |
| Suffix | GTCA |
| Enzyme out | BsmBI |
| Resistance | Kanamycin |
| Assembly Standard | 3.0 |
| Owner | |
| Public | True |
| Genbank_file | GB_UA_257A |
| SBOL_file | GB_UA_257A |
| View GenBank Features | |
| Component Elements | ||
|---|---|---|
| ID | Name | Type |
| GB0030 | pP35S | PROM+UTR+ATG |
| GB_UD_4952 | pUPD2:NLS-COM1 (B3-B4) | CDS |
| GB01186 | 3xNLS-VP64 AD | CT |
| GB0037 | pTnos | TER |
You can use your favorite sequence viewer to open the genbank file. We suggest to use Benchling.
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