GB_UA_258C
35S:scFv-VP64:Tnos (TU)
| Description: | GB1592. TU for the expression of the scFv (recognizes the SunTag epitope) fused to the VP64 domain. Author: JB. |
|---|---|
| Reference: | Tanenbaum, M. E., Gilbert, L. A., Qi, L. S., Weissman, J. S., & Vale, R. D. (2014). A protein-tagging system for signal amplification in gene expression and fluorescence imaging. Cell, 159(3), 635-646. |
| General Information | |
|---|---|
| ID | GB_UA_258C |
| Name | 35S:scFv-VP64:Tnos |
| Function | TU |
| Category | TU |
| Level | 1-alpha |
| Vector | pDGB3_alpha1 |
| Prefix | GGAG |
| Suffix | GTCA |
| Enzyme out | BsmBI |
| Resistance | Kanamycin |
| Assembly Standard | 3.0 |
| Owner | |
| Public | True |
| Genbank_file | GB_UA_258C |
| SBOL_file | GB_UA_258C |
| View GenBank Features | |
| Component Elements | ||
|---|---|---|
| ID | Name | Type |
| GB0030 | pP35S | PROM+UTR+ATG |
| GB_UD_4B08 | pUPD2_ScFV | CDS |
| GB01186 | 3xNLS-VP64 AD | CT |
| GB0037 | pTnos | TER |
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